Effects of nitrogen and phosphorus on anatoxin-a, homoanatoxin-a, dihydroanatoxin-a and dihydrohomoanatoxin-a production by Phormidium autumnale

Heath, Mark W.; Wood, Susanna A.; Barbieri, Rafael F.; Young, Roger G.; Ryan, Ken G.

doi:10.1016/j.toxicon.2014.10.014

Cited by 30 publications

(32 citation statements)

References 33 publications

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“…Therefore, the wide distribution of dihydro derivatives with respect to the ATX-a and homoATX-a observed in our results points to their natural occurrence. Anatoxin-a is transformed into a non-toxic compound after sunlight exposure, and the dihydro derivatives show an approximately 10-fold reduction of affinity for nicotinic receptors and lower toxicity [2,55]. Anatoxin-a is transformed into a non-toxic compound after sunlight exposure, and the dihydro derivatives show an approximately 10-fold reduction of affinity for nicotinic receptors and lower toxicity [2,55].…”

Section: Resultsmentioning

confidence: 99%

Monitoring of freshwater toxins in European environmental waters by using novel multi‐detection methods

Rodríguez

Fraga-Corral

Alfonso

et al. 2016

Enviro Toxic and Chemistry

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Monitoring the quality of freshwater is an important issue for public health. In the context of the European project μAqua, 150 samples were collected from several waters in France, Germany, Ireland, Italy, and Turkey for 2 yr. These samples were analyzed using 2 multitoxin detection methods previously developed: a microsphere-based method coupled to flow-cytometry, and an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. The presence of microcystins, nodularin, domoic acid, cylindrospermopsin, and several analogues of anatoxin-a (ATX-a) was monitored. No traces of cylindrospermopsin or domoic acid were found in any of the environmental samples. Microcystin-LR and microcystin-RR were detected in 2 samples from Turkey and Germany. In the case of ATX-a derivatives, 75% of samples contained mainly H2 -ATX-a and small amounts of H2 -homoanatoxin-a, whereas ATX-a and homoanatoxin-a were found in only 1 sample. These results confirm the presence and wide distribution of dihydro derivatives of ATX-a toxins in European freshwaters. Environ Toxicol Chem 2017;36:645-654. © 2016 SETAC

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Section: Resultsmentioning

confidence: 99%

Monitoring of freshwater toxins in European environmental waters by using novel multi‐detection methods

Rodríguez

Fraga-Corral

Alfonso

et al. 2016

Enviro Toxic and Chemistry

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“…3 for treatment abbreviations. that N fixation could have been occurring (Heath et al 2014, Wood et al 2017, McAllister et al 2018.…”

Section: Phormidium Biomass Accrualmentioning

confidence: 99%

“…Investigators have suggested that elevated NO 3 2 concentrations enhance Phormidium growth dur-ing early accrual stages, but that internal mat nutrientcycling processes in established mats allow it to grow independently of water-column nutrient concentrations (McAllister et al 2016, 2018, Wood et al 2017. Studies of the effect of NO 3 2 on Phormidium growth have been limited to laboratory-culture-based (Heath et al 2014 and observational studies (Heath et al 2011, Wood et al 2017, McAllister et al 2018. Observational studies are complicated by covariation of other physicochemical factors that make extraction of meaningful relationships difficult.…”

mentioning

confidence: 99%

The effects of velocity and nitrate on Phormidium accrual cycles: a stream mesocosm experiment

et al. 2018

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Proliferations of benthic cyanobacteria in the genus Phormidium are a global concern because of their increasing prevalence and ability to produce harmful toxins. Most studies have been observational and have linked physicochemical variables to Phormidium cover measured at the reach scale. Authors of these studies have alluded to nutrients and flow as key factors in accrual. Our goal was to use an experimental approach to examine how changes in velocity and NO 3 2 concentrations influence Phormidium accrual. We hypothesized that: 1) Phormidium biomass accrual would be positively correlated with stream velocity; 2) biomass accrual would be positively related to NO 3 2 concentration, which would have a stronger effect during early accrual; 3) an NO 3 2 Â velocity interaction would arise from saturation of accrual at high NO 3 2 and high velocity; and 4) the probability of detachment would increase with decreasing velocity. We assessed mat expansion, biomass (as phycoerythrin and chlorophyll a [Chl a] concentrations, and biovolume), and algal assemblage composition in flow-through channel mesocosms for 16 d. We crossed 2 velocity treatments (0.1 and 0.2 m/s, slow and fast, respectively) with 3 NO 3 2 treatments (0.02, 0.1, and 0.4 mg/L, ambient, medium, and high, respectively). Velocity was positively correlated with all measures of Phormidium biomass, but patch expansion rates increased at similar rates across all treatments. NO 3 2 had no effect during early accrual, but phycoerythrin concentrations increased with increasing NO 3 2 in fast-velocity treatments. At the end of the experiment, patch size was greater in the high-velocity treatments because of a greater number of partial or full patch detachments in slow-velocity treatments. These results suggest that NO 3 2 concentrations do not affect Phormidium expansion and detachment, but may be important during colonization (not investigated), and that mat expansion occurs at a similar rate regardless of velocity, but termination of accrual cycles occurs earlier in slow velocities.

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“…A new approach that has been developed for culture‐based studies on benthic species is to inoculate many individual culture vessels with a known wet weight of starting material and at each time point an entire culture vessel is sacrificed for analysis. The sample is homogenised and subsampled for cell counts and extra‐ and intracellular cyanotoxin analysis (Harland, Wood, Moltchanova, Williamson, & Gaw, 2013; Heath, Wood, Barbieri, Young, & Ryan, 2014; Heath, Wood, Young, & Ryan, 2016). These culture studies have been instrumental in demonstrating that toxin production varies with growth stage and is influenced by nutrient availability.…”

Section: Autecology Of Toxic Benthic Cyanobacteriamentioning

confidence: 99%

“…These culture studies have been instrumental in demonstrating that toxin production varies with growth stage and is influenced by nutrient availability. For example, anatoxin quotas in M. autumnalis were highest during the early exponential phase suggesting that anatoxins may provide a physiological benefit during initial substrate colonisation (Heath et al., 2014; Heath et al, 2016). In batch culture experiments, the slow‐growing benthic species Scytonema cf.…”

Section: Autecology Of Toxic Benthic Cyanobacteriamentioning

confidence: 99%

Toxic benthic freshwater cyanobacterial proliferations: Challenges and solutions for enhancing knowledge and improving monitoring and mitigation

et al. 2020

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This review summarises knowledge on the ecology, toxin production, and impacts of toxic freshwater benthic cyanobacterial proliferations. It documents monitoring, management, and sampling strategies, and explores mitigation options. Toxic proliferations of freshwater benthic cyanobacteria (taxa that grow attached to substrates) occur in streams, rivers, lakes, and thermal and meltwater ponds, and have been reported in 19 countries. Anatoxin‐ and microcystin‐containing mats are most commonly reported (eight and 10 countries, respectively). Studies exploring factors that promote toxic benthic cyanobacterial proliferations are limited to a few species and habitats. There is a hierarchy of importance in environmental and biological factors that regulate proliferations with variables such as flow (rivers), fine sediment deposition, nutrients, associated microbes, and grazing identified as key drivers. Regulating factors differ among colonisation, expansion, and dispersal phases. New ‐omics‐based approaches are providing novel insights into the physiological attributes of benthic cyanobacteria and the role of associated microorganisms in facilitating their proliferation. Proliferations are commonly comprised of both toxic and non‐toxic strains, and the relative proportion of these is the key factor contributing to the overall toxin content of each mat. While these events are becoming more commonly reported globally, we currently lack standardised approaches to detect, monitor, and manage this emerging health issue. To solve these critical gaps, global collaborations are needed to facilitate the rapid transfer of knowledge and promote the development of standardised techniques that can be applied to diverse habitats and species, and ultimately lead to improved management.

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Effects of nitrogen and phosphorus on anatoxin-a, homoanatoxin-a, dihydroanatoxin-a and dihydrohomoanatoxin-a production by Phormidium autumnale

Cited by 30 publications

References 33 publications

Monitoring of freshwater toxins in European environmental waters by using novel multi‐detection methods

Monitoring of freshwater toxins in European environmental waters by using novel multi‐detection methods

The effects of velocity and nitrate on Phormidium accrual cycles: a stream mesocosm experiment

Toxic benthic freshwater cyanobacterial proliferations: Challenges and solutions for enhancing knowledge and improving monitoring and mitigation

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