The experiment was conducted at the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj. Prayagraj during the session 2023 - 2024. The experiment was laid out in randomized block design with three replications, and the study consists of nine treatment combinations including control by using different Effect of Trichoderma asperelloides (TA41) on production and quality of strawberry. The best treatment was T8 (TA 41 – 80ml/15 L) & T7 (TA 41 – 70 ml/15 L) which shows highest values in all the parameters viz., number of leaf/plant (30.72), plant spread N-S (cm) (31.31 cm), plant spread E-W (cm) (35.31 cm), Days taken to first flower initiation (22.29), Days taken to initiation of fruit set (29.07), Days taken to first harvest (54.02), Days taken to final harvest (91.53), Number of pickings (10.98), Fruit length (mm) (55.46 mm), Fruit width (38.89 mm), Fresh weight (g) (40.36), Number of fruit/plant (16.92), Fruit yield/plant (g) (682.91), Fruit yield/ha. (61.46 q), TSS (11.55 brix), Vitamin C (52.76 mg/100g), Acidity (1.96 %). All the treatments were significantly superior in their growth, flowering, fruit yield and quality of strawberry over control (T0) and (T8).