Effects of nitrogen removal microbes and partial nitrification-denitrification in the integrated vertical-flow constructed wetland

Fu, Guiping; Yu, Tianyu; Ning, Kaile; Guo, Zhipeng; Wong, Ming Hung

doi:10.1016/j.ecoleng.2016.06.054

Cited by 54 publications

(19 citation statements)

References 39 publications

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“…Our previous studies showed that penguin or seal activities led to generally low C:N ratios, which had been used as an indicator for the intensity of penguin or seal activities in maritime Antarctica ( Wang Q. et al, 2019 ). The abundances of nitrifiers and denitrifiers in soils were significantly correlated with C:N ratios in previous study ( Fu et al, 2016 ). The negative correlations of the lg values of nirS and nirK gene abundances with C:N ratios ( Figure 3 ) indicated that sea animal activities, which changed tundra soil C:N ratios, had an important effect on the denitrifier abundances in tundra soils.…”

Section: Discussionsupporting

confidence: 64%

“…The negative correlations of the lg values of nirS and nirK gene abundances with C:N ratios ( Figure 3 ) indicated that sea animal activities, which changed tundra soil C:N ratios, had an important effect on the denitrifier abundances in tundra soils. Many environmental variables (e.g., temperature, pH, salinity, dissolved oxygen, organic matter, and dissolved inorganic nitrogen) could affect the abundances of soil nirS and nirK genes ( Zheng et al, 2015 ; Fu et al, 2016 ; Gao et al, 2016 ; Li et al, 2019 ; Shi et al, 2019 ). However, in this study, nirS and nirK gene abundances were not significantly related with other environmental variables ( Supplementary Table S1 ), possibly due to the low number of soil sample replicates within the same type of tundra soils.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Sea Animal Activities on Tundra Soil Denitrification and nirS‐ and nirK-Encoding Denitrifier Community in Maritime Antarctica

et al. 2020

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In maritime Antarctica, sea animals, such as penguins or seals, provide a large amount of external nitrogen input into tundra soils, which greatly impact nitrogen cycle in tundra ecosystems. Denitrification, which is closely related with the denitrifiers, is a key step in nitrogen cycle. However, effects of sea animal activities on tundra soil denitrification and denitrifier community structures still have received little attention. Here, the abundance, activity, and diversity of nirS‐ and nirK-encoding denitrifiers were investigated in penguin and seal colonies, and animal-lacking tundra in maritime Antarctica. Sea animal activities increased the abundances of nirS and nirK genes, and the abundances of nirS genes were significantly higher than those of nirK genes ( p < 0.05) in all tundra soils. Soil denitrification rates were significantly higher ( p < 0.05) in animal colonies than in animal-lacking tundra, and they were significantly positively correlated ( p < 0.05) with nirS gene abundances instead of nirK gene abundances, indicating that nirS-encoding denitrifiers dominated the denitrification in tundra soils. The diversity of nirS-encoding denitrifiers was higher in animal colonies than in animal-lacking tundra, but the diversity of nirK-encoding denitrifiers was lower. Both the compositions of nirS‐ and nirK-encoding denitrifiers were similar in penguin or seal colony soils. Canonical correspondence analysis indicated that the community structures of nirS‐ and nirK-encoding denitrifiers were closely related to tundra soil biogeochemical processes associated with penguin or seal activities: the supply of nitrate and ammonium from penguin guano or seal excreta, and low C:N ratios. In addition, the animal activity-induced vegetation presence or absence had an important effect on tundra soil denitrifier activities and nirK-encoding denitrifier diversities. This study significantly enhanced our understanding of the compositions and dynamics of denitrifier community in tundra ecosystems of maritime Antarctica.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Effects of Sea Animal Activities on Tundra Soil Denitrification and nirS‐ and nirK-Encoding Denitrifier Community in Maritime Antarctica

et al. 2020

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“…In the studied CW system the nitrogen removal efficiency was lower due to low influent C/N ratio (TOC/TN < 1). High nitrogen removal rate assumes higher TOC/TN ratios (2-4) as shown with the integrated vertical-flow constructed wetland [75]. It is possible that due to low C/N ratio a significant amount of nir genes were not expressed.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Bacterial Community Abundance and Structure in Horizontal Subsurface Flow Wetland Mesocosms Treating Municipal Wastewater

Oopkaup

Truu

Nõlvak

et al. 2016

Water

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Dynamics of bacterial community abundance and structure of a newly established horizontal subsurface flow (HSSF) pilot-scale wetland were studied using high-throughput sequencing and quantitative polymerase chain reaction (PCR) methods. Bacterial community abundance increased rapidly within one month and stabilised thereafter in three replicate HSSF constructed wetland (CW) mesocosms. The most dominant phylum was Proteobacteria, followed by Bacteroidetes in wetland media biofilms and Firmicutes in influent wastewater. CW bacterial community diversity increased over time and was positively related to the wastewater treatment efficiency. Increase in the abundance of total bacteria in the community was accompanied with the abundance of denitrifying bacteria that promoted nitrate and nitrite removal from the wastewater. During the 150-day study period, similar patterns of bacterial community successions were observed in replicate HSSF CW mesocosms. The data indicate that successions in the bacterial community in HSSF CW are shaped by biotic interactions, with a significant contribution made by external abiotic factors such as influent chemical parameters. Network analysis of the bacterial community revealed that organic matter and nitrogen removal in HSSF CW could be, in large part, allocated to a small subset of tightly interconnected bacterial species. The diversity of bacterial community and abundance of denitrifiers were good predictors of the removal efficiency of ammonia, nitrate and total organic C in HSSF CW mesocosms, while the removal of the seven-day biochemical oxygen demand (BOD 7 ) was best predicted by the abundance of a small set of bacterial phylotypes. The results suggest that nitrogen removal in HSSF CW consist of two main pathways. The first is heterotrophic nitrification, which is coupled with aerobic denitrification and mediated by mixotrophic nitrite-oxidizers. The second pathway is anaerobic denitrification, which leads to gaseous intermediates and loss of nitrogen as N 2 .

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“…IVCW consists of a down-flow tank and an up-flow tank. This U-shaped flow structure gives rise to the metagenic "aerobic-anoxic-anaerobic-anoxic-aerobic" multifunctional layers [3]. The structure of the influent tank allows it to function as an aerobiotic zone, whereas the effluent tank is an anaerobic zone [4].…”

Section: Introductionmentioning

confidence: 99%

Methane Emissions Driven by Adding a Gradient of Ethanol as Carbon Source in Integrated Vertical-Flow Constructed Wetlands

Liu¹,

Wang

et al. 2019

Water

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This work aims to investigate the methane emissions from integrated vertical-flow constructed wetlands (IVCWs) when ethanol is added as an external carbon source. In this study, a gradient of ethanol (0, 2, 4, 8, 16 and 32 mmol/L) was added as the carbon source in an IVCW planted with Cyperus alternifolius L. The results showed that the methane emission flux at an ethanol concentration of 32 mmol/L was 32.34 g CH4 m−2 day−1 less than that of the control experiment (0 mmol/L) and that the methane emission flux at an ethanol concentration of 16 mmol/L was 5.53 g CH4 m−2 day−1 less than that at 0 mmol/L. In addition, variations in the water quality driven by the different ethanol concentrations were found, with a redox potential range of −64 mV to +30 mV, a pH range of 6.6–6.9, a chemical oxygen demand (COD) removal rate range of 41% to 78%, and an ammonia nitrogen removal rate range of 59% to 82% after the ethanol addition. With the average CH4-C/TOC (%) value of 35% driven by ethanol, it will be beneficial to understand that CH4-C/TOC can be considered an ecological indicator of anthropogenic methanogenesis from treatment wetlands when driven by carbon sources or carbon loading. It can be concluded that adding ethanol as an external carbon source can not only meet the water quality demand of the IVCW treatment system but also stimulate and increase the average CH4 emissions from IVCWs by 23% compared with the control experiment. This finding indicates that an external carbon source can stimulate more CH4 emissions from IVCWs and shows the importance of carbon sources during sewage treatment processes when considering greenhouse emissions from treated wetlands.

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Effects of nitrogen removal microbes and partial nitrification-denitrification in the integrated vertical-flow constructed wetland

Cited by 54 publications

References 39 publications

Effects of Sea Animal Activities on Tundra Soil Denitrification and nirS‐ and nirK-Encoding Denitrifier Community in Maritime Antarctica

Effects of Sea Animal Activities on Tundra Soil Denitrification and nirS‐ and nirK-Encoding Denitrifier Community in Maritime Antarctica

Dynamics of Bacterial Community Abundance and Structure in Horizontal Subsurface Flow Wetland Mesocosms Treating Municipal Wastewater

Methane Emissions Driven by Adding a Gradient of Ethanol as Carbon Source in Integrated Vertical-Flow Constructed Wetlands

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