BackgroundAn attempt has been made to explore the nutritional profile of pink oyster mushrooms that have been grown in various agricultural residues, like sugarcane bagasse, rice straw, coconut coir, sawdust, along with other nutrient supplements such as Defatted mustard and Chickpea powder, for appropriate growth and fruiting body formation in a short span of time. The spawn production was experimented with five different grain varieties. The study became interesting when the observation was a bit different from the traditional practices where the addition of defatted mustard was supplemented and resulted in a positive correlation in reducing the fruiting time, improved yield, and the nutritional profile of Pleurotus djamor.ResultsAn elevated yield of 651.93 g/kg was recorded in the medium where the RS and DM were used in the ratio of 1: 0.01 (Rice straw +1% w/w Defatted mustard) bag, whereas in terms of protein content, a maximum yield of 32.57 ± 0.79 mg/g was observed when SB: DM was in the same ratio (Sugarcane bagasse +1% w/w Defatted mustard) bag.ConclusionTo confer the best outcomes from the screened substrates, a series of experiments were performed by varying the concentration of RS, SB, with 1% w/w DM. It is worth mentioning that the highest protein content of 32.76 ± 0.38 mg/g was obtained along with the total yield of 702.56 ± 2.9 g/kg of mushroom when the ratio of RS: SB was 0.7: 0.3.This article is protected by copyright. All rights reserved.