Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

Sepsis is a clinical condition caused by an uncontrolled response to an infection, leading to acute kidney injury (AKI) and an increased risk of mortality. Although life support and antibiotic therapy are available, the mortality rate remains high in patients with sepsis. The present study investigated the therapeutic effect of glutamine on gentamicin-induced acute kidney injury in Sprague-Dawley rats. We randomly grouped 24 male rats to the normal control, AKI (control), glutamine 50 mg/kg, and glutamine 500 mg/kg groups. The dose was administered orally for 14 consecutive days. Rats treated with glutamine 500 mg/kg showed changes in systolic blood pressure. Glutamine increased renal blood flow, creatinine clearance, and the levels of potassium, creatinine, blood urea nitrogen, and urine osmolality, while reducing the relative excretion of sodium, potassium, urinary sodium, and plasma blood urea nitrogen and creatinine levels. In our study, glutamine supplementation reduced gentamicin-induced oxidative stress and increased catalase, superoxide dismutase, glutathione peroxidase, and glutathione levels in AKI rats. In addition, glutamine supplementation attenuated the severity of pathological features in this model. Collectively, our results showed that gentamicin has therapeutic potential against gentamicin-induced AKI due to its ability to mitigate the effects of oxidative stress.

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“…RBF was measured using a previously reported method [19]. The left renal vein and artery were dissected.…”

Section: Methodsmentioning

confidence: 99%

Glutamine alleviates the renal dysfunction associated with gentamicin‐induced acute kidney injury in Sprague–Dawley rats

Zhan

Wang

Zhang

et al. 2021

Biotech and App Biochem

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“…Accordingly, renal creatinine clearance (Cr), as well as the absolute and relative excretion of sodium and potassium were calculated. Kidney tissue was stained by Hematoxylin and Eosin (H&E) staining for histological study; antioxidant capacity was measured by FRAP and lipid peroxidation by MDA for biochemical study [12,13].…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Vitamin C Concomitant Treatment on Deferasirox-induced Renal Toxicity in Rats

et al. 2021

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Background and Aim: Deferasirox (Exjade) is an iron-chelating drug used in patients with beta-thalassemia major. Oxidative stress is among f the major causes of nephrotoxicity and its progression. Deferasirox, due to oxidative stress and increased cell apoptosis causes the dysfunction of renal tubules and renal toxicity. According to its antioxidant and anti-inflammatory properties, the present study explored the effect of vitamin C on deferasirox-induced kidney damage. Methods & Materials: This study was performed on 30 Wistar rats in 3 groups of control, deferasirox, and deferasirox plus vitamin C. To induce the nephrotoxicity, the intra-peritoneum injection of deferasirox (75 mg/kg/day) was used. After taking plasma from the blood samples of the explored rats, we determined the values of Cr, Na+, K+, Mg+, osmolality, and BUN in the obtained plasma and urine samples. The creatinine clearance, as well as the relative and absolute excretion of sodium and potassium, were also calculated. After separating the two kidneys, they were used for the histologic study with Hematoxylin and Eosin (H&E) staining, as well as Malondialdehyde (MDA) and Ferric Reducing Antioxidant Power (FRAP) biochemical studies. Ethical Considerations This study was approved by the Research Ethics Committee of Arak University of Medical Sciences (Code: IR.ARAKMU.REC.1396.309). Results: Cotreatment with deferasirox and vitamin C reduced renal tissue MDA and relative and absolute Na and K excretion and urine osmolarity; this method also increased creatinine clearance and renal tissue FRAP. Conclusion: The co-administration of vitamin C presented a significant protective effect on the renal toxicity induced by deferasirox. The protective property of deferasirox is because of the antioxidant impacts of vitamin C in reducing oxidative stress and lipid peroxidation.

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“…Also, the osmolality was measured in blood and urine samples. The observed values were used to Calculate Creatinine Clearance (CCR), absolute excretion of sodium (UNaVo) and potassium (UKVo), and fractional excretion of sodium (FENa) and potassium (FEK) using the suggested equations [17]. On the other hand, the kidneys were delivered to the pathology laboratory; the left and right kidneys were used to determine the tissue damage and measure Malondialdehyde (MDA) with FRAP test, respectively.…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Co-Treatment With Hydroethanolic Extract of Origanum Vulgare on Gentamicin-Induced Renal Toxicity in Rats

Hajihashemi

Rajabi

Farahani

2020

cmja

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Objective: Renal toxicity and ototoxicity are considered as the main side effects of aminoglycoside antibiotics, such as gentamicin. The present study aimed to investigate the effect of co-treatment by origanum vulgare extract on the gentamicin-induced renal toxicity. Methods: Adult male Wistar rats in the weight range of 200 to 250 grams were randomly assigned into four groups (n = 8): control, renal toxicity (with the intraperitoneal injection of gentamicin [100 mg/kg/day], for eight days), co-treatment with OV extract and gentamicin vehicle (with the intraperitoneal injection of normal saline and OV extract gavage [40 mg/kg], for eight days), co-treatment with OV ethanolic extract (with the intraperitoneal injection of gentamicin [100 mg/kg/day] and OV extract gavage [40 mg/kg]), for eight days. The amount of urea, creatinine, sodium, potassium, and osmolality were measured in the plasma and urine samples. The left kidney was used for the histological study and the right kidney was used to measure MDA and FRAP. Results: treatment with OV ethanolic extract significantly decreased the blood concentrations of creatinine, urea, the absolute excretion of sodium, the fractional excretion of sodium and potassium, and MDA, compared with the renal toxicity group. Besides, co-treatment with ethanolic extract of origanum Vulgare significantly increased creatinine clearance, urinary osmolality, and FRAP, compared with the renal toxicity group. Conclusion: The oral co-treatment with ethanolic extract of origanum vulgare has a protective effect on gentamicin-induced renal toxicity. This effect can be induced by reducing the oxidative stress caused by free radicals and reducing the amount of lipid peroxidation caused by gentamicin.

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Effects of Nitroglycerine on Renal Ischemia-Reperfusion Injury in Adult Male Rats

Cited by 6 publications

References 36 publications

Glutamine alleviates the renal dysfunction associated with gentamicin‐induced acute kidney injury in Sprague–Dawley rats

Glutamine alleviates the renal dysfunction associated with gentamicin‐induced acute kidney injury in Sprague–Dawley rats

Protective Effects of Vitamin C Concomitant Treatment on Deferasirox-induced Renal Toxicity in Rats

Protective Effects of Co-Treatment With Hydroethanolic Extract of Origanum Vulgare on Gentamicin-Induced Renal Toxicity in Rats

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