Effects of non-steroidal antiallergic eyedrops on the complement-mediated histamine release from human cells

Goldschmidt, Pablo; Couturier, Christine S.; Haeffner‐Cavaillon, Nicole; Kazatchkine, M. D.; Herman, D.

doi:10.3109/09273949709085045

Cited by 7 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there are a few reports that suggest levocabastine inhibits histamine release similar as our present results do. For instance, Tasaka et al 2) and Goldschmidt et al 17) found that levocabas- Histamine content in tears was measured 30 min after antigen application. Levocabastine and pemirolast were instilled at 5 and 15 min before antigen challenge into the bilateral eye at 5 ml/site.…”

Section: Discussionmentioning

confidence: 99%

Increasing Effect by Simultaneous Use of Levocabastine and Pemirolast on Experimental Allergic Conjunctivitis in Rats

Minami

Hossen

Kamei

2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Levocabastine is a potent H 1 -receptor antagonist which provides long-acting antihistaminic and antiallergic activities in experimental animals. 1,2) In experimental allergic conjunctivitis in guinea pigs, levocabastine causes the inhibition of antigen-and histamine-induced conjunctivitis, and these effects are superior to those of cromolyn sodium.1) Clinical studies also revealed that levocabastine is well-tolerated and is at least as effective as cromolyn sodium for the treatment of pollen-provoked conjunctivitis.3,4) Abelson et al. 5) also found that levocabastine is significantly more effective than cromolyn sodium in inhibiting itching, hyperemia, eyelid swelling, chemosis and tearing in allergic conjunctivitis induced by ocular allergen challenge.On the other hand, the treatment of conjunctivitis associated with pollinosis is at present carried out by antihistamines either alone or in combination with alpha-adrenergic agents and mast cell stabilizers. 6) Pemirolast is a drug that exhibits potent antiallergic activity compared with cromolyn sodium.7) In addition, it inhibits histamine release induced by immunological and non-immunological stimuli, and this effect is more potent than that of cromolyn sodium and tranilast. Therefore, it is possible to obtain a more effective medical treatment of allergic conjunctivitis if levocabastine and pemirolast are administered simultaneously. In the present study, we investigated the effect of the simultaneous use of levocabastine and pemirolast on eye scratching behavior and allergic symptoms in allergic rat conjunctivitis models which we recently developed. 8) MATERIALS AND METHODS AnimalsSix weeks-old male Wistar rats were obtained from Japan SLC, Inc., Shizuoka, Japan. The animals were housed in an air-conditioned room maintained at 24Ϯ2°C with humidity of 55Ϯ15%. They were given standard laboratory rodent chow (Oriental Yeast, Tokyo, Japan) and water ad libitum.Reagents The following reagents were obtained from the sources shown in parentheses: egg albumin (Grade VII; crystallized and lyophilized, essentially salt-free, Sigma, St. Louis, MO, U.S.A.), aluminum hydroxide hydrate gel (alum, LSL, Tokyo, Japan), and Evans blue (Wako, Tokyo, Japan). Bordetella pertussis inactive microorganism suspension (B. pertussis) was kindly provided by the Kitasato Institute Research Center for Biologicals, Saitama, Japan. The following drugs were purchased from the companies indicated: 0.025% levocabastine hydrochloride eye drops (levocabastine, Livostin eye drops, Santen Pharmaceutical Co., Osaka, Japan) and 0.1% pemirolast potassium ophthalmic solution (pemirolast, Alegysal ophthalmic solution, Santen Pharmaceutical Co., Osaka, Japan). Levocabastine and pemirolast were instilled at 5 and 15 min, respectively, before antigen challenge into the bilateral eyes at 5 ml/site.Sensitization The rats were sensitized by injection of 0.6 ml of physiological saline containing egg albumin (1 mg), alum (2 mg) and 10 10 B. pertussis into the four footpads on the first day. Five days later, t...

show abstract

Section: Discussionmentioning

confidence: 99%

Increasing Effect by Simultaneous Use of Levocabastine and Pemirolast on Experimental Allergic Conjunctivitis in Rats

Minami

Hossen

Kamei

2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

Complement inhibition reduces material‐induced leukocyte activation with PEG modified polystyrene beads (Tentagel™) but not polystyrene beads

Gorbet

Sefton

2005

J Biomedical Materials Res

View full text Add to dashboard Cite

With isolated leukocytes, inhibiting complement reduced material-induced leukocyte activation (CD11b) with polyethylene glycol modified polystyrene beads (PS-PEG), but not with polystyrene beads (PS). The PS-PEG beads (TentaGel) were complement activating as measured by SC5b-9 levels consistent with the sensitivity of these beads to leukocyte inhibition with complement inhibitors. Following contact with PS and PS-PEG beads, isolated leukocytes in plasma and in the absence in platelets were found to significantly upregulate CD11b, while TF expression and exposure of phosphatidylserine remained at background levels. Complement inhibition by means of sCR1 partially reduced CD11b upregulation on PS-PEG beads, but had no effect with PS beads. Pyridoxal-5-phosphate (P5P) was able to significantly reduce both CD11b upregulation and exposure of phosphatidylserine with PS-PEG beads, although it did not appear to inhibit SC5b-9 production. Pentamidine and NAAGA inhibited complement and were effective in reducing CD11b upregulation with both PS and PS-PEG. However, they also had an inhibitory effect on leukocyte signaling mechanisms, precluding their utility for further study in this context. Leukocyte adhesion occurred to similar extents on both PS and PS-PEG beads. While sCR1 and P5P blocked adhesion and activation (for adherent leukocytes) on PS-PEG beads, they had no effect on leukocytes adherent to PS beads. The role of complement in leukocyte activation and adhesion was found to be material-dependent. Thus, leukocyte-material compatibility may be resolved by complement inhibition in some but not all cases. For these other materials (example here was PS), other mechanisms, such as fibrinogen adsorption and direct leukocyte release, may need exploitation to minimize leukocyte activation and adhesion.

show abstract

N -Acetyl-aspartyl-glutamic acid inhibits cellular recruitment and mediator release during the late allergen-induced nasal reaction

Miadonna

Milazzo

Salmaso

et al. 1998

European Journal of Clinical Pharmacology

View full text Add to dashboard Cite

show abstract

Effects of non-steroidal antiallergic eyedrops on the complement-mediated histamine release from human cells

Cited by 7 publications

References 20 publications

Increasing Effect by Simultaneous Use of Levocabastine and Pemirolast on Experimental Allergic Conjunctivitis in Rats

Increasing Effect by Simultaneous Use of Levocabastine and Pemirolast on Experimental Allergic Conjunctivitis in Rats

Complement inhibition reduces material‐induced leukocyte activation with PEG modified polystyrene beads (Tentagel™) but not polystyrene beads

N -Acetyl-aspartyl-glutamic acid inhibits cellular recruitment and mediator release during the late allergen-induced nasal reaction

Contact Info

Product

Resources

About