Effects of noncontingent ethanol, DHEA, and pregnanolone administration on ethanol self-administration in outbred female rats

Erwin, Laura; Nilges, Mark R.; DeLarge, Alyssa F.; Weed, Peter F.; Winsauer, Peter J.

doi:10.1016/j.alcohol.2018.05.003

Cited by 2 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One- and two-way repeated measures analysis of variance (ANOVA) tests were used to analyze the grouped data, and Dunnett’s post hoc tests were used to make pairwise comparisons with control (saline or THC/RMBT vehicle) when significant main effects or interactions were obtained. When the number of subjects varied across doses and prohibited a repeated measures analysis (e.g., safety concerns prevented the testing of some doses and dose combinations), a 95% confidence interval for heroin alone was generated and data that fell outside of the 95% confidence interval were considered statistically significant (Erwin, Nilges, DeLarge, Weed, & Winsauer, 2019). As part of ensuring that the doses tested in each animal captured comparably effective dose ranges (i.e., no effect to a maximally effective dose), the subjects were not always tested with the same number of doses and this reduced the sample size for some dosages.…”

Section: Methodsmentioning

confidence: 99%

Opioid-enhancing antinociceptive effects of delta-9-tetrahydrocannabinol and amitriptyline in rhesus macaques.

Nilges¹,

Bondy²,

Grace³

et al. 2020

Experimental and Clinical Psychopharmacology

Self Cite

View full text Add to dashboard Cite

Cannabinoids can enhance the antinociceptive effects of opioids in a synergistic manner, potentially reducing the analgesic dosage of opioids and improving pain therapy. This strategy has also been used as a rationale to combine certain antidepressants and opioids. In this experiment, opioid-induced thermal antinociception was assessed in rhesus macaques using a warm-water tail-withdrawal procedure with 3 water temperatures (40, 50, and 55 °C). In general, the acute antinociceptive effects of intramuscular (i.m.) cumulative doses of heroin were studied alone or in combination with i.m. (-)-trans-delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), or the tricyclic antidepressant amitriptyline. A nonantinociceptive dose of THC (1 mg/kg) shifted the ED50 for the heroin dose-effect curve 3.6-fold leftward at 50 °C and 1.9-fold leftward at 55 °C compared with heroin alone. When the cannabinoid type-1 receptor (CB1R) antagonist, rimonabant, was administered prior to the most effective THC-heroin combination, rimonabant blocked the THC enhancement of heroin antinociception. When CBN (1–3.2 mg/kg) was administered prior to heroin, or 1 mg/kg of CBN was administered prior to a combination of 0.32 mg/kg of THC and heroin, no shifts were evident in the heroin dose-effect curves at either temperature. However, similar to THC, amitriptyline (0.32–1 mg/kg) administered prior to heroin significantly shifted the heroin dose-effect curve leftward. Heroin produced both dose- and temperature-dependent thermal antinociception in nonhuman primates and THC produced opioid-enhancing effects in a CB1R-dependent manner. These effects of THC were not shared by cannabinol, but were quantitatively similar to that of amitriptyline.

show abstract

Section: Methodsmentioning

confidence: 99%