Effects of Notch2 on proliferation, apoptosis and steroidogenesis in bovine luteinized granulosa cells

Li, Yating; Jing, Jiongjie; Dang, Wenqing; Han, Qi; Guo, Xiangyu; Jia, Kaiqi; Cheng, Ying; Wang, Kai; Kebreab, E.; Lyu, Lihua

doi:10.1016/j.theriogenology.2021.05.009

Cited by 8 publications

(5 citation statements)

References 38 publications

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“…Notch2 controls GC proliferation by regulating c-Myc expression [12]. Notch2 silencing downregulated the cell cycle genes CCND1, CCND2, and CDK4 and upregulated p21, which is consistent with previous findings in bovine luteinized GCs [29]. Furthermore, the Notch2-mediated inhibition of the Notch pathway caused apoptosis in GCs by upregulating Bax, Caspase-3, and p21 and downregulating Bcl-2, similar to the way that the Wnt signaling pathway regulates GC apoptosis.…”

Section: Discussionsupporting

confidence: 90%

“…DAPT downregulated Hey2 in fetal and neonatal mouse ovaries [50]. In addition, the shRNA-mediated knockdown of Notch2 in bovine luteinized GCs decreased the mRNA and protein expression of Notch2 and the relative expression of Hes1 and Hey2 [29].…”

Section: Discussionmentioning

confidence: 91%

“…A negative control (NC) was synthesized using the following primers: sense, 5 ′ -GATCCGTTCTCCGA-ACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAACTTTTTTG-3 ′ and antisense, 5 ′ -AATTCAAAAAAGTTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACG-TTCGGA GAACG-3. Lentiviral packaging and centrifugation were performed as described previously [29]. The GCs were cultured in 6-well plates until 60% confluence was reached and were transfected in medium containing a final concentration of 10 µg/mL of polybrene.…”

Section: Treatment Of Gcsmentioning

confidence: 99%

“…Follicle-stimulating hormone (FSH) regulates β-catenin protein and Wnt2 mRNA expression in bovine GCs, suggesting the involvement of Wnt/β-catenin signaling in follicular development and ovarian steroidogenesis in cattle [3]. The Notch2 receptor and Jagged2 ligand are expressed in bovine GCs and thecal cells, participating in luteinized GC development [29]. These results imply that Wnt/β-catenin and Notch signaling are synergistically regulated in bovine follicular GCs.…”

Section: Introductionmentioning

confidence: 98%

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Notch2 Regulates the Function of Bovine Follicular Granulosa Cells via the Wnt2/β-Catenin Signaling Pathway

Dang,

Ren,

Chen

et al. 2024

Animals

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Ovarian follicular GCs are strongly implicated in the growth, development, and atresia of ovarian follicles. The Wnt/β-catenin and Notch signaling pathways participate in GC proliferation, differentiation, apoptosis, and steroid hormone production during follicular development. However, the crosstalk between Wnt and Notch signaling in GCs remains unclear. This study investigated this crosstalk and the roles of these pathways in apoptosis, cell cycle progression, cell proliferation, and steroid hormone secretion in bovine follicular GCs. The interaction between β-catenin and Notch2 in GCs was assessed by overexpressing CTNNB1, which encodes β-catenin. The results showed that inhibiting the Notch pathway by Notch2 silencing in GCs arrested the cell cycle, promoted apoptosis, reduced progesterone (P4) production, and inhibited the Wnt2-mediated Wnt/β-catenin pathway in GCs. IWR-1 inhibited Wnt2/β-catenin and Notch signaling, reduced GC proliferation, stimulated apoptosis, induced G1 cell cycle arrest, and reduced P4 production. CTNNB1 overexpression had the opposite effect and increased 17β-estradiol (E2) production and Notch2 protein expression. Co-immunoprecipitation assays revealed that Notch2 interacted with β-catenin. These results elucidate the crosstalk between the Wnt/β-catenin and Notch pathways and the role of these pathways in bovine follicular GC development.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 91%

Section: Treatment Of Gcsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Notch2 Regulates the Function of Bovine Follicular Granulosa Cells via the Wnt2/β-Catenin Signaling Pathway

Dang,

Ren,

Chen

et al. 2024

Animals

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“…The BCL-2/ BAX ratio determines cell survival or death. 50 The results of this study showed that, in the low-HAX-1 expression HA1800 group, the expressions of Ki67 and c-Myc were upregulated, and the BCL-2/BAX ratio was increased. Contrasting results were obtained from the high-HAX-1 expression HA1800 group.…”

Section: Discussionmentioning

confidence: 65%

Effect of different expression patterns of HAX-1 on the proliferation and apoptosis of human astrocyte

Xia¹,

Zhu²,

Zhang³

et al. 2022

Adv Clin Exp Med

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Background. Spinal cord injury (SCI), a serious damage of the central nervous system, has become an extremely important issue that threatens the health of people worldwide. The proliferation of astrocytes plays an important role in the repair of SCI, which has typical two-sided effects. The HS1-associated protein X-1 (HAX-1), plays an important role in the physiological and pathological processes of cell apoptosis, proliferation, migration, and invasion. However, the specific role and mechanism of HAX-1 in human astrocyte HA1800 are still unclear. Objectives.To explore the effect of HAX-1 on the proliferation and apoptosis of HA1800 cells and preliminarily explore its possible underlying mechanism. Materials and methods.The HA1800 cell lines with high-and low-expression levels of HAX-1 were established using lentiviral vector pcDNA3. 1. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot were employed to determine the expression of HAX-1 after transfection. Cell viability and proliferation ability were estimated using MTT and 5-Ethynyl-2'deoxyuridine (EdU) assay. The effects of HAX-1 on the HA1800 cell cycle and apoptosis were determined using flow cytometry. The BCL-2/BAX ratio and the expression of Ki67 and c-Myc in the transfected cells were detected using qRT-PCR. The Gene Expression Profiling Interactive Analysis (GEPIA) database was used to determine the relationships of HAX-1, BAX and BCL-2.Results. The HA1800 cell lines with high and low expression of HAX-1 were obtained. The MTT, EdU and flow cytometry showed that elevated HAX-1 could inhibit the proliferation, reduce the viability and promote the apoptosis of HA1800 cells. The qRT-PCR showed that the mRNA levels of Ki67, c-Myc and the BCL-2/ BAX ratio were significantly decreased in the HAX-1 high-expression group, but increased in the HAX-1 low-expression group. The results from the GEPIA database showed that HAX-1 was positively correlated with BAX and BCL-2 in the spinal cord.Conclusions. The HAX-1 may influence the biological behavior of human HA1800 cells due to the progression of cell cycle and apoptosis associated with BCL-2/BAX.

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Androgens and Notch signaling cooperate in seminiferous epithelium to regulate genes related to germ cell development and apoptosis

Kamińska,

Lustofin,

Brzoskwinia

et al. 2024

Reproductive Biology

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Effects of Notch2 on proliferation, apoptosis and steroidogenesis in bovine luteinized granulosa cells

Cited by 8 publications

References 38 publications

Notch2 Regulates the Function of Bovine Follicular Granulosa Cells via the Wnt2/β-Catenin Signaling Pathway

Notch2 Regulates the Function of Bovine Follicular Granulosa Cells via the Wnt2/β-Catenin Signaling Pathway

Effect of different expression patterns of HAX-1 on the proliferation and apoptosis of human astrocyte

Androgens and Notch signaling cooperate in seminiferous epithelium to regulate genes related to germ cell development and apoptosis

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