Effects of nucleotides and nucleosides on the activity of cyclic AMP phosphodiesterase from rat brain.

Murakami, M.T.; Miura, Shuichi; Ito, Isao; Gotoh, Noriko; Nakamichi, Hiroyuki

doi:10.1620/tjem.141.23

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Although caffeine is known to be a PDE inhibitor, 9 its PDE inhibitory effect is weak and the K i is in the range of several millimolar. 19 The results from our studies suggest that, in the case of coffee, the PDEinhibiting potential of caffeine has been overestimated in the past.…”

Section: ■ Results and Discussionmentioning

confidence: 70%

Identification of a Phosphodiesterase-Inhibiting Fraction from Roasted Coffee (Coffea arabica) through Activity-Guided Fractionation

Röhrig

Liesenfeld

Richling

2017

J. Agric. Food Chem.

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Recent reports that coffee can significantly inhibit cAMP phosphodiesterases (PDEs) in vitro, as well as in vivo, have described another beneficial effect of coffee consumption. However, the PDE-inhibiting substances remain mostly unknown. We chose activity-guided fractionation and an in vitro test system to identify the coffee components that are responsible for PDE inhibition. This approach indicated that a fraction of melanoidins reveals strong PDE-inhibiting potential (IC = 130 ± 42 μg/mL). These melanoidins were characterized as water-soluble, low-molecular weight melanoidins (<3 kDa) with a nitrogen content of 4.2% and a carbohydrate content lower than those of other melanoidins. Fractions containing known PDE inhibitors such as chlorogenic acids, alkylpyrazines, or trigonelline as well as N-caffeoyl-tryptophan and N-p-coumaroyl-tryptophan did not exert PDE-inhibiting activity. We also observed that the known PDE inhibitor caffeine does not contribute to the PDE-inhibiting effects of coffee.

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Section: ■ Results and Discussionmentioning

confidence: 70%

Identification of a Phosphodiesterase-Inhibiting Fraction from Roasted Coffee (Coffea arabica) through Activity-Guided Fractionation

Röhrig

Liesenfeld

Richling

2017

J. Agric. Food Chem.

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“…All three presumptive intracellular phenomena associated with pineal c~l-adrenoceptors, i.e. phosphatidylinositol hydrolysis (Smith et aL, 1979), cGMP synthesis (Zatz, 1982) and PGEe release (Ritta and Cardinali, 1982), could be implicated in pineal PDE activation inasmuch as: (i) some products ofphosphatidylinositol metabolism are very effective in stimulating cyclic nucleotide PDE partially purified from bovine brain (Tai and Tai, 1982); (ii) cGMP at low concentrations stimulates rat brain PDE activity (Murakami et aL, 1983); (iii) PGE2, which increases pineal cAMP levels (Ritta and Cardinali, 1981), may augment low-Km PDE activity by a substrate-mediated effect (Oleshansky and Neff, 1975;Minneman and Iversen, 1976 a). Additionally other changes, like modification of affinity of fl-adrenoceptor sites or of coupling of these receptors with adenylate cyclase could be involved (Leblanc and Cianarello, 1984).…”

Section: Discussionmentioning

confidence: 99%

Interaction between ?- and ?-adrenoceptors in rat pineal adenosine cyclic 3?,5?-monophosphate phosphodiesterase activation

Vacas

Sarmiento

Cardinali

1985

J. Neural Transmission

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The adrenergic regulation of the low-Km pineal cAMP phosphodiesterase (PDE) activity was studied in adult female rats. PDE activity showed a transient enhancement (up to 42%) during the process of degeneration of pineal sympathetic nerve terminals that followed superior cervical ganglionectomy (SCGx), thus confirming the neural modulation of the enzyme. Treatment with isoproterenol (0.3-5.0 mg/Kg) increased significantly PDE activity within 2 hours. Phenylephrine induced a significant increase of pineal PDE only at a 10 mg/Kg dose, while at a lower dose (1 mg/Kg) it potentiated the stimulatory effect of isoproterenol. Treatment of pineal organ cultures with 100 microM propranolol inhibited norepinephrine (NE)-induced PDE activity while 100 microM phentolamine had no significant effect. Propranolol at doses unable to alter the in vitro NE-induced stimulation of pineal PDE activity (1 microM), antagonized such NE effect when used in combination with 1 microM phentolamine. At equimolecular concentrations (1 microM) the mixed alpha-beta-adrenergic agonist NE was more effective than the beta-adrenergic agonist isoproterenol to increase pineal PDE in vitro. These results suggest an alpha-beta-adrenergic interaction in the sympathetic modulation of low-Km PDE activity of rat pineal gland.

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Susceptibility to ischemic insult in hypertensive rats: Correlation between degree of ischemia and hypertension.

Katayama¹,

Sugimoto²,

Inamura³

et al. 1986

Jpn Circ J

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Effects of nucleotides and nucleosides on the activity of cyclic AMP phosphodiesterase from rat brain.

Cited by 3 publications

References 8 publications

Identification of a Phosphodiesterase-Inhibiting Fraction from Roasted Coffee (Coffea arabica) through Activity-Guided Fractionation

Identification of a Phosphodiesterase-Inhibiting Fraction from Roasted Coffee (Coffea arabica) through Activity-Guided Fractionation

Interaction between ?- and ?-adrenoceptors in rat pineal adenosine cyclic 3?,5?-monophosphate phosphodiesterase activation

Susceptibility to ischemic insult in hypertensive rats: Correlation between degree of ischemia and hypertension.

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