Effects of nutrient-poor and anaerobic conditions on maintenance of R100-1 and RSF2124 plasmids in model populations of Escherichia coli.

Wang, Pichao; Ohtake, Hisao; Toko, Kiyoshi

doi:10.1264/microbes1986.4.67

Bulletin of Japanese Society of Microbial Ecology

1989

DOI: 10.1264/microbes1986.4.67

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Effects of nutrient-poor and anaerobic conditions on maintenance of R100-1 and RSF2124 plasmids in model populations of Escherichia coli.

Pichao Wang

Hisao Ohtake

Kiyoshi Toko

Abstract: Little is known about factors affecting plasmid maintenance in natural populations of bacteria. To study the effects of limitation of carbon and energy sources on plasmid maintenance, the fate of 8100-1 and RSF2124 plasmids in model populations of Escherichia coli was investigated under nutrient-poor and anaerobic conditions. Nutrient-poor and anaerobic conditions adversely affected the maintenance of the plasmids. In batch culture experiments the plasmid loss was observed during the logarithmic phase of growt… Show more

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Cited by 3 publications

References 19 publications

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Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Asakura

Toko

1993

Bulletin of Japanese Society of Microbial Ecology

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Effect of solid particles on transfer of plasmid R100-1 from Escherichia coli LC102 to a rifampicin-resistant mutant of E. coli DHl was investigated using nutritionally-rich (L medium) and-poor medium (supernatant of a mixed liquor). Plasmid transfer was most significantly inhibited in the presence of bentonite. The addition of 2g.1-1 bentonite decreased the specific rate of transconjugant increase in mating to one ninetieth in the former medium, and one twenty-third in the latter. Activated sludge decreased the rates to one third and one fifth, respectively, whereas sterilized activated sludge did not affect the rates.

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Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Asakura

Toko

1993

Bulletin of Japanese Society of Microbial Ecology

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Application of multi-stage continuous culture for estimating net rate of plasmid transfer.

Toko¹,

Yao²,

Ohtake³

1990

J. Gen. Appl. Microbiol.

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In connection with the assessment of the safety of genetically-engineered microorganisms released to natural environments (2), data concerning the frequency of plasmid transmission have been accumulated under various environmental conditions. The frequency of plasmid transfer is conventionally defined as the number of transconjugants divided by the number of donors after a fixed incubation period (S). However, the increase in the number of transconjugants is a result of not only plasmid transfer but also transconjugant growth. It must be noted that this may lead to overestimation of plasmid transmissibility, particularly when the growth rate is high, relative to the rate of plasmid transfer.In the present study, we demonstrate a method of multi-stage continuous culture for determining the net rate of plasmid transfer. This method allows us to estimate the relative contributions of plasmid transfer and growth to the increase in the number of transconjugants.If the law of mass action is applied to the process of conjugal plasmid transfer from donor to recipient cells, the increase of transconjugant concentration (NT) with mating time (t) in a continuous culture at a dilution rate (D) is written as dNT/dt = kptNDNR + /TNT -L>NT (1) where kpt denotes the rate constant for plasmid transfer; ND and NR are the concentrations of donor and recipient cells; /T is the specific growth rate of transconjugants. At a steady state,

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Theoretical and methodological studies of continuous microbial bioreactors

Toko

2003

J. Gen. Appl. Microbiol.

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This article reviews most of the author's studies on process development and reactor design for continuous microbial reactions. (1) Enzyme reactions of growing and non-growing microbial cells immobilized in agar gel beads were analyzed pertaining to the effects of external and internal diffusion of substrate on reaction kinetics. (2) Experimental correlations of production rates of beta-fructosidase and acid phosphatase with dilution rate of continuous culture were simulated based on an operon model for enzyme regulation. (3) Population dynamics of an amylase-producing bacteria and their mutant were discussed in relation to enzyme productivity in a continuous culture of spore-forming bacteria. (4) Plasmid mobilization in a mixed population of donor, recipient, and helper cells was investigated in a continuous culture as a model study of accidental release of a genetically modified plasmid into a natural environment. (5) A production rate increase of up to 100-fold was achieved by cell-recycle culturing of continuous acetic acid fermentation using a filter module with a hollow fiber membrane. (6) The feasibility of a continuous surface culture for the biooxidation of organic substances was ascribed to an enhanced oxygen absorption rate in the presence of a microbial film on a liquid surface. (7) Simultaneous separation of inhibitory products using an electrodialysis module during some organic acid fermentations was effective for increasing production in a continuous culture.

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Effects of nutrient-poor and anaerobic conditions on maintenance of R100-1 and RSF2124 plasmids in model populations of Escherichia coli.

Cited by 3 publications

References 19 publications

Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Application of multi-stage continuous culture for estimating net rate of plasmid transfer.

Theoretical and methodological studies of continuous microbial bioreactors

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