Effects of obesity on the healing of bone fracture in mice

Gao, Feng; Lv, Tianrun; Zhou, Jiahui; Qin, Xiaodong

doi:10.1186/s13018-018-0837-7

Cited by 41 publications

(28 citation statements)

References 40 publications

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“…Some authors hold that fat tissue can exert osteoprotective influences [25][26][27]. In contrast, other researchers document that overweight or obesity impede bone growth and development [28,29], or simply accelerate osteoresorption [30,31]. In the current study, it was observed that the dietary-induced obesity (DIO) in adult females (SHO-FAT group) lowered BMC and BMD in relation to SHO females treated with the control diet (SHO-CON).…”

Section: Discussionmentioning

confidence: 53%

Bone losses in obese, ovariectomized rats appear to be independent from sclerostin-induced inhibition of the Wnt/β-catenin pathway

Radzki¹,

Bieńko²,

Filip³

et al. 2020

Ann Agric Environ Med.

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Introduction. Overweight and obesity, as well as a gonadal function, are pivotal factors influencing bone tissue metabolism. Materials and method. The aim of the study was to determine the effect of dietary induced obesity (DIO) on bone tissue metabolism in sham-operated (SHO) or ovariectomized (OVX) adult female Wistar rats. Additionally, the influence of DIO in SHO or OVX on the concentration of sclerostin in the blood serum was analyzed. After SHO or OVX, the rats were placed in groups (n=8) and either received a standard diet (11.5 MJ/kg) (SHO-CON; OVX-CON) or a high-energy diet (17.6 MJ/kg) (SHO-FAT; OVX-FAT). The experiment lasted for 90 days and allowed for the establishment of osteopenia in OVX females and obesity in the rats that had received the high-energy diet. Results. The results of the study demonstrate that obesity or/and ovariectomy increases the resorption of femora and tibiae, hence decreasing the densitometric and mechanical parameters affecting the bone structure in adult females rats. The strongest osteodegenerative effect was seen in the OVX-FAT females. Interestingly, the degree of bone tissue degradation caused exclusively by ovariectomy was similar to that found in the obese sham-operated rats. Conclusions. Bone losses invoked by DIO seem to be independent from the Wnt/β-catenin pathway inhibition induced by sclerostin. While further study is necessary, the obtained results suggest that the usage of sclerostin anti-body in the treatment of osteoporosis can be ineffective, and in obese patients the undertaking of such therapy should be reassessed.

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Section: Discussionmentioning

confidence: 53%

Bone losses in obese, ovariectomized rats appear to be independent from sclerostin-induced inhibition of the Wnt/β-catenin pathway

Radzki¹,

Bieńko²,

Filip³

et al. 2020

Ann Agric Environ Med.

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“…Moreover, obesity significantly limited fracture repair, with no callus or connected bone observed in obese mice days after fracture unlike control animals. 81 High levels of limb fractures are also observed in obese children, [82][83][84] where abnormal BMAT and adiponectin levels may be contributing to reduced bone strengthen and delayed repair.…”

Section: Role Of Adiponectin In the Regulation Of Bone Homeostasismentioning

confidence: 99%

Adiponectin signalling in bone homeostasis, with age and in disease

et al. 2021

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Adiponectin is the most abundant circulating adipokine and is primarily involved in glucose metabolism and insulin resistance. Within the bone, osteoblasts and osteoclasts express the adiponectin receptors, however, there are conflicting reports on the effects of adiponectin on bone formation and turnover. Many studies have shown a pro-osteogenic role for adiponectin in in vivo murine models and in vitro: with increased osteoblast differentiation and activity, alongside lower levels of osteoclastogenesis. However, human studies often demonstrate an inverse relationship between adiponectin concentration and bone activity. Moreover, the presence of multiple isoforms of adiponectin and multiple receptor subtypes has the potential to lead to more complex signalling and functional consequences. As such, we still do not fully understand the importance of the adiponectin signalling pathway in regulating bone homeostasis and repair in health, with age and in disease. In this review, we explore our current understanding of adiponectin bioactivity in the bone; the significance of its different isoforms; and how adiponectin biology is altered in disease. Ultimately, furthering our understanding of adiponectin regulation of bone biology is key to developing pharmacological and non-pharmacological (lifestyle) interventions that target adiponectin signalling to boost bone growth and repair in healthy ageing, following injury or in disease.

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“…X-ray and CT scan X-ray examination and CT examination analysis have been widely used to assess fracture healing. The right femur X-ray image was taken on days 7, 14, and 21 following the procedure using the Faxitron system (Faxitron X-ray, Wheeling, IL, USA) [24,25]. The right femur was examined using a Viva CT 40…”

Section: Tissue Preparationmentioning

confidence: 99%

Different effects of Wnt/β-catenin activation and PTH activation in adult and aged male mice metaphyseal fracture healing

Liu

Qin

Yang

et al. 2020

Preprint

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Background: Fractures in older men are not uncommon and need to be healed as soon as possible to avoid related complications. Anti-osteoporotic drugs targeting Wnt/β-catenin and PTH (parathyroid hormone) to promote fracture healing have become an important direction in recent years. The study is to observe whether there is a difference in adult and aged situations by activating two signal paths. Methods: A single cortical hole with a diameter of 0.6 mm was made in the femoral metaphysis of Catnb lox(ex3) mice and wild-type mice. The fracture healing effects of CA (Wnt/β-catenin activation) and PTH (activated by PTH (1–34) injections) were assessed by X-ray and CT imaging on days 7, 14, and 21 after fracture. The mRNA levels of β-catenin, PTH1R( Parathyroid hormone 1 receptor ), and RUNX2(Runt-related transcription factor 2) in the fracture defect area were detected using RT-PCR. Angiogenesis and osteoblasts were observed by immunohistochemistry and osteoclasts were observed by TRAP (Tartrate-resistant Acid Phosphatase). Result: Adult CA mice and adult PTH mice showed slightly better fracture healing than adult wild-type (WT) mice, but there was no statistical difference. Aged CA mice showed better promotion of angiogenesis and osteoblasts and better fracture healing than aged PTH mice. Conclusion: The application of Wnt/β-catenin signaling pathway drugs for fracture healing in elderly patients may bring better early effects than PTH signaling pathway drugs, but the long-term effects need to be observed.

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Effects of obesity on the healing of bone fracture in mice

Cited by 41 publications

References 40 publications

Bone losses in obese, ovariectomized rats appear to be independent from sclerostin-induced inhibition of the Wnt/β-catenin pathway

Bone losses in obese, ovariectomized rats appear to be independent from sclerostin-induced inhibition of the Wnt/β-catenin pathway

Adiponectin signalling in bone homeostasis, with age and in disease

Different effects of Wnt/β-catenin activation and PTH activation in adult and aged male mice metaphyseal fracture healing

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