Effects of oestrogen on progesterone synthesis and arachidonic acid metabolism in human luteal cells

Abstract

Oestradiol, at relatively high concentrations, is a potent inhibitor of basal and hCG induced luteal cell steroidogenesis in vitro. No support is provided for the concept that luteolysis is mediated by local production of PGF2 alpha. The putative luteolytic effect of oestradiol may entail reduced metabolism of arachidonic acid to lipoxygenase derived products by luteal cells rather than direct stimulation of prostaglandin production by itself.

Estrogens and androgens affect human luteal cell function

“…In human CL a luteolytic role has been previously hypothesized for E 2 , able to negatively influence in vitro P luteal release (6,7,25). Interestingly our results represent the first demonstration that in human CL this effect is not specific for E 2 , both E 1 and estriol are also able to negatively affect basal and hCG-induced P release.…”

“…The PGF 2a has been suggested to mediate the inhibitory action of E 2 in primate CL (27), whereas Fisch et al (6) and Thibier et al (28) did not confirm this hypothesis in human LC. In agreement with these investigators, our data suggest a lack of any involvement of PGs in E 2 luteolytic effect in human LC.…”

“…Actually E 2 has been demonstrated to suppress basal and hCG-induced steroidogenesis in human luteal cells (LC) (6,7). E 2 could initiate luteolysis during the midluteal phase, when an increase in E 2 luteal level occurs (1).…”

Identification of inflammatory and proresolving lipid mediators in bone marrow and their lipidomic profiles with ovariectomy and omega‐3 intake

“…Ovariectomy in rodents and menopause in women not only results in reduced estrogen levels, but also leads to changes in the glucocorticoid response to stress [22][23][24]. Estrogens and glucocorticoids regulate the activities of cyclooxygenase (COX) and LOX [25][26][27][28][29][30]. Changes in progenitor cell number and fate also occur following surgical menopause [31][32][33] and altered lipid mediator synthesis may contribute to these changes.…”

Inductive effect of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibiscus macranthus on in vitro production of estradiol

“…Modulation by estradiol of progesterone synthesis in defined human luteal cells have been demonstrated (Vega et al, 1994;Fish et al, 1994). The data of both studies indicate direct inhibition of 3␤-hydroxysteroid deshydrogenase activity by estradiol.…”