Effects of oils and oil-related substrates on the synthetic activity of membrane-bound lipase from Rhizopus chinensis and optimization of the lipase fermentation media

Wang, Dong; Xu, Yuanyuan; Shan, Tianyu

doi:10.1016/j.bej.2008.03.003

Cited by 74 publications

(49 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar RSM using both nutritional and physiological factors have been reported by Kaushik et al [7] and Wang et al [13]. However, Sharma et al [14] used only three physical parameters i.e.…”

Section: Resultsmentioning

confidence: 68%

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

View full text Add to dashboard Cite

Aspergillus terreus produces lipase 7.01 IU/ml in 96 h after optimization by one variable at a time method. Using the significant factors i.e. corn oil (A), sodium nitrate (B), casein (C), agitation rate (D) and incubation period (E) RSM was carried out resulting in 19.65 IU/ml from the combination ?1(A), -1(B), -1(C), ?1(D) and 0(E). The interactions between sodium nitrate, casein and agitation with corn oil were most significant. Scale up of production from 250 ml shake flask to 30 l bioreactor resulted in increased productivity of 0.52 IU/ml/h as against 0.2 IU/ ml/h obtained in shake flasks. This lipase could carryout solvent free synthesis of partial glycerides of oleic acid with 96% efficiency in 12 h.

show abstract

Section: Resultsmentioning

confidence: 68%

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Lipidic carbon sources serve as inducers, vegetable oils have high contents of oleic and palmitic acid and has been reported as a good inducer for lipase production by many bacterial and fungal strains (Wang et al, 2008;Odeyemi et al, 2014). It was observed that further increase in oil concentration did not favour lipase production in this work, which might be due to poor oxygen transfer as explained by PereiraMeirelles et al, (2005).…”

Section: Effects Of Metals and Heavy Metals On The Growth And Lipase supporting

confidence: 29%

Bioaccumulation of Heavy Metals and Optimization of Lipase Production by Lysinibacillus sphaericus Strain ODE16_EKITI Isolated from Domestic Oil-Rich Wastewater

Adebayo¹,

Mustapha²

2017

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…ACIKEL et al (2011) described that variation of Tween 80 concentration enhanced the lipase activity of R. delemar about two-fold. Span-80 (Sorbitan oleate) had the best results enhancing the lipase activity from R. chinensis (WANG et al, 2008). …”

Section: Transesterification Screeningmentioning

confidence: 99%

“…As result, the lipase levels from corn oil medium was three fold higher than olive, palm and canola oils, and soy oil has the second best activity. Rhizopus delemar lipase had a maximum activity when sunflower oil was included in the fermentation medium (ACIKEL et al 2011). WANG et al (2008 observed that the effect of olive oil was the best on the growth and lipase activity.…”

Section: Transesterification Screeningmentioning

confidence: 99%

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

Facchini

Vici

Pereira

et al. 2016

Revista Brasileira De Engenharia De Biossistemas

View full text Add to dashboard Cite

The application of lipases in various fields has been notably increased in the last few decades and qualitative/quantitative improvements need to be done. However, many methodologies of screening are described in order to find a good lipase producer and statistical optimization is a necessary tool to improve lipase production. In this work, an isolation of filamentous fungi lipase producers and a transesterification capacity screening was evaluated. Four fungi were chosen to the transesterification reaction assays and the best fungus selected was submitted to a submerged fermentation. Parameters of the culture medium were optimized using response surface methodology. Selected liquid medium was SR at 30 °C, 72 h, 100 rpm. Corn oil was the best carbon source and together with Tween 80 increased two-fold the lipase activity. After the experimental design, the new medium optimized were 3.5-fold higher than the original liquid medium and was composed by 0.5% corn oil, 0.012% MgSO 4 .7H 2 O, 0.015% KH 2 PO 4 , 0.05% NH 4 H 2 PO 4 . Hence, the lipase produced proved its transesterification capacity and can be used for biodiesel production. Keywords: Lipase; Transesterification; Biodiesel UMA METODOLOGIA ÚTIL PARA SELECIONAR LIPASES COM ATIVIDADE DE TRANSESTERIFICAÇÃO VISANDO APLICAÇÃO NA PRODUÇÃO DE BIODIESEL RESUMOA aplicação de lipases em vários campos tem aumentado notavelmente nas últimas décadas e melhorias qualitativas/quantitativas precisam ser feitas. Entretanto, a fim de encontrar um bom produtor de lipase muitas metodologias de seleção são descritas, além disso, a otimização estatística é uma ferramenta necessária para melhorar a produção de lipases. Este trabalho apresenta o isolamento de fungos filamentosos produtores de lipase e a avaliação da capacidade de transesterificação dessas enzimas. Quatro fungos foram selecionados para os ensaios de transesterificação e o melhor fungo isolado foi submetido a fermentação submersa. Parâmetros do meio de cultivo foram otimizados usando metodologia de superfície de resposta. O meio de cultivo líquido selecionado foi o SR, a 30 °C, 72 h e 100 rpm. Óleo de milho foi a melhor fonte de carbono e, quando adicionado de Tween 80 aumentou duas vezes a atividade de lipase. Após o planejamento experimental, o novo meio de cultivo otimizado, composto por * polizeli@ffclrp.usp.br Brazilian Journal of Biosystems Engineering v. 10(1): 01-13, 2016 2 óleo de milho 0,5%, extrato de levedura 0,45%, peptona 0,2%, MgSO 4 .7H 2 O 0,012%, KH 2 PO 4 0,015% e NH 4 H 2 PO 4 0,05%, foi 3,5 vezes melhor que o original. Dessa forma, a lipase produzida demonstrou capacidade de transesterificação e pode ser utilizada para a produção de biodiesel.

show abstract

Effects of oils and oil-related substrates on the synthetic activity of membrane-bound lipase from Rhizopus chinensis and optimization of the lipase fermentation media

Cited by 74 publications

References 31 publications

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

Bioaccumulation of Heavy Metals and Optimization of Lipase Production by Lysinibacillus sphaericus Strain ODE16_EKITI Isolated from Domestic Oil-Rich Wastewater

A Useful Methodology to Select Lipase-Catalyzed Transesterification Aiming Biodiesel Application

Contact Info

Product

Resources

About