Effects of Ooplasm Manipulation on DNA Methylation and Growth of Progeny in Mice1

Cheng, Yong; Wang, Kai; Kellam, Lori D.; Lee, Young S.; Liang, Cheng-Guang; Han, Zhiming; Mtango, Namdori R.; Latham, Keith E.

doi:10.1095/biolreprod.108.073593

Cited by 37 publications

(61 citation statements)

References 64 publications

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“…No long-term effects on growth of offspring or phenotypic abnormalities were observed when intra- and interstrain cytoplasmic transfers were compared [91]. When mitochondrial concentrates were injected into mouse 2 pronuclear stage embryos, there was an increase in progression to blastocyst stage with no untoward effects [92].…”

Section: The Safety and Efficacy Of Cytoplasmic Or Mitochondrial mentioning

confidence: 99%

The Role of Mitochondria from Mature Oocyte to Viable Blastocyst

Chappel

2013

Obstetrics and Gynecology International

199

133

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The oocyte requires a vast supply of energy after fertilization to support critical events such as spindle formation, chromatid separation, and cell division. Until blastocyst implantation, the developing zygote is dependent on the existing pool of mitochondria. That pool size within each cell decreases with each cell division. Mitochondria obtained from oocytes of women of advanced reproductive age harbor DNA deletions and nucleotide variations that impair function. The combination of lower number and increased frequency of mutations and deletions may result in inadequate mitochondrial activity necessary for continued embryo development and cause pregnancy failure. Previous reports suggested that mitochondrial activity within oocytes may be supplemented by donor cytoplasmic transfer at the time of intracytoplasmic sperm injection (ICSI). Those reports showed success; however, safety concerns arose due to the potential of two distinct populations of mitochondrial genomes in the offspring. Mitochondrial augmentation of oocytes is now reconsidered in light of our current understanding of mitochondrial function and the publication of a number of animal studies. With a better understanding of the role of this organelle in oocytes immediately after fertilization, blastocyst and offspring, mitochondrial augmentation may be reconsidered as a method to improve oocyte quality.

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Section: The Safety and Efficacy Of Cytoplasmic Or Mitochondrial mentioning

confidence: 99%

The Role of Mitochondria from Mature Oocyte to Viable Blastocyst

Chappel

2013

Obstetrics and Gynecology International

199

133

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“…Future research in a large-animal system like cattle should also assess the potential of OT to affect paternal genome function and for more subtle phenotypic changes or transient effects [50][51][52].…”

Section: Further Perspectivesmentioning

confidence: 99%

Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line1

Ferreira

Burgstaller

Perecin

et al. 2010

Biology of Reproduction

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Ooplasmic transfer (OT) has been used in basic mouse research for studying the segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that the moderate amount of mitochondria introduced by OT is transmitted to the offspring's oocytes; e.g., modifies the germ line. The donor mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in the female germ line. Centrifugation-mediated enrichment of mitochondria in the pole zone of the recipient zygote's ooplasm and its substitution by donor ooplasm led to elevated proportions of donor mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% +/- 9.6% versus 12.1% +/- 4.5%; P < 0.0001). We also characterized the proliferation of mitochondria from the OT parents-the recipient zygote (Bos primigenius taurus type) and the donor ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from the seven OT fetuses at different points during fetal development found a decreasing proportion of donor mtDNA (r(2) = 0.78). This indicates a preferred proliferation of recipient taurine mitochondria in the context of the nuclear genotype of the OT recipient expressing a B. primigenius indicus phenotype.

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“…The abovementioned studies proposed that mtDNA copy number is an exciting new paradigm for embryo quality assessment. The importance of mitochondrial threshold in oocyte competence is also shown on many animal models including mouse, 84 pig, 85 and cattle. 52 …”

Section: Nation Of Array-comparative Genomic Hybridization (Acgh) Rementioning

confidence: 91%

Mitochondrial dysfunction and ovarian aging

Wang

Zhang

Jiang

et al. 2017

American J Rep Immunol

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Mitochondria are double‐membrane‐bound organelles that are responsible for the generation of most of the cell's energy. Mitochondrial dysfunction has been implicated in cellular senescence in general and ovarian aging in particular. Recent studies exploited this association by studying mitochondrial DNA (mtDNA) copy number as a potential biomarker of embryo viability and the use of mitochondrial nutrients and autologous mitochondrial transfer as a potential treatment for poor ovarian function and response.

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Effects of Ooplasm Manipulation on DNA Methylation and Growth of Progeny in Mice1

Cited by 37 publications

References 64 publications

The Role of Mitochondria from Mature Oocyte to Viable Blastocyst

The Role of Mitochondria from Mature Oocyte to Viable Blastocyst

Pronounced Segregation of Donor Mitochondria Introduced by Bovine Ooplasmic Transfer to the Female Germ-Line1

Mitochondrial dysfunction and ovarian aging

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