Effects of orally administered clodronate on biochemical markers of bone turnover in ovariectomized rats

Abstract

Orally administered clodronate was evaluated for its ability to inhibit increase of bone turnover in ovariectomized (OVX) rats. Six -month~ old GVX Sprague-Dawley rats were administered by gavage a vehicle (veh) or clodronate (clod) at doses of 100 or 500 mg/kg/d five times a week. Sham-operated rats received the vehicle. Treatment began on the day of ovariectomy and continued for 3 months. At the end of the study, blood and 24-hour urine samples were collected from fasted rats. Serum osteocalcin (S-OC) and alkaline phosphatase (S-ALP) were determined by RIA and by a colorimetric method, respectively. Urinary excretion of free deoxypyridinoline was measured using an ELISA assay-(Pyrilinks-D, Metra Biosystems) and expressed as the ratio to urinary creatinine (DPD/Cr). Statistical analyses were performed by analysis of variance. Comparisons were carried out using linear contrasts. Results are given as means (SE) and corresponding p values: