Effects of Orengedoku-to and Senkanmeimoku-to, Traditional Herbal Medicines, on the Experimental Herbal Medicines, on the Experimental Elevation of Aqueous Flare in Pigmental Rabbits

Abstract

We investigated the effects of Orengedoku-to (Huanglian-Jie-Du-Tang in Chinese) and Senkanmeimoku-to (Xygan-Ming-Mu-Tang in Chinese), traditional herbal medicines, on experimantal elevation of aqueous flare in pigmented rabbits. To produce the elevation of aqueous flare in rabbits, prostaglandin E2 (PGE2) was applied to the cornea with use of a glass cylinder, or lipopolysaccharides (LPS) were injected into the ear vein. Animals were pretreated by the oral administration of 150 g/day of food containing 0.7%, 0.2% or 0.07% (w/w) Orengedoku-to, or 2%, 0.6% or 0.2% (w/w) Senkanmeimoku-to for 5 days. Aqueous flare was measured with a laser flare-cell meter. Pretreatment with 0.7% or 0.2% Orengedoku-to and 2% Senkanmeimoku-to did suppress significantly (P < 0.05) elevation of aqueous flare induced by PGE2. Pretreatment with 0.7% or 0.2% Orengedoku-to and 2% or 0.6% Senkanmeimoku-to significantly suppressed (P < 0.001) elevation of aqueous flare induced by LPS. It is possible that Orengedoku-to and Senkanmeimoku-to may migrate some forms of uveitis.

An Herbal Medicine Orengedokuto Prevents Indomethacin-Induced Enteropathy

“…47) OGT influences microcirculatory blood flow, which is one of the mechanisms responsible for the efficacy of OGT. 48) Finally, there are many reports that OGT has anti-inflammatory effects, [49][50][51][52] such as the ability to inhibit rat paw oedema. Efforts to identify the active ingredients of OGT and the properties of each ingredient are now under way.…”

Effects of Oral Administration of Gardeniae fructus Extract and Intravenous Injection of Crocetin on Lipopolysaccharide- and Prostaglandin E2-induced Elevation of Aqueous Flare in Pigmented Rabbits

“…6). Nagaki et al (2001a) reported that oral administration of Orengedoku-to or Scutellariae radix extract both inhibited LPS-and PGE 2 -induced elevation of aqueous flare in pigmented rabbits. Nagaki et al (2001b) further reported that Scutellariae radix extract, and its components (baicalein, baicalin and wogonin) strongly inhibited LPS-induced elevation of aqueous flare in pigmented rabbits, but did not inhibit PGE 2 -induced flare.…”

“…To produce the experimental elevation of aqueous flare, transcorneal diffusions of PGE 2 were performed, as described previously (Nagaki et al, 2001a). A glass cylinder, 11 mm in diameter, was placed on the right cornea of an unanesthetized rabbit.…”

“…have previously reported that intravenous injection of lipopolysaccharide (LPS) produced aqueous flare elevation in pigmented rabbits, and that Sairei-to (Cai-Ling-Tang in Chinese), a traditional herbal medicine, inhibited aqueous flare elevation. Nagaki et al (2001a) have demonstrated that Orengedoku-to (Huanglin-Jie-Du-Tang in Chinese) inhibited both LPS-and prostaglandin E 2 (PGE 2 )-induced elevation of aqueous flare in pigmented rabbits. Orengedoku-to contains the extracts of four medicinal herbs: Scutellariae radix, Coptidis rhizoma, Phellodendri cortex and Gardeniae fructus (Table 1).…”

In vivo and in vitro inhibitory effects of a traditional Chinese formulation on LPS-stimulated leukocyte–endothelial cell adhesion and VCAM-1 gene expression

“…In vivo animal or in vitro studies on the anti-inflammatory effects of HLJDT have been carried out in a great variety of acute and chronic inflammatory diseases, either pathogenic microbesrelated or not. Based upon research results from Medline databases, these could be divided into four categories as (1) infection diseases: gastroenteritis, in vitro antimicrobial activity (Franzblau and Cross, 1986), intestinal parasite infection (Yang et al, 1996), acne (Higaki et al, 1996), gastric ulcer (Takase et al, 1989;Ohta et al, 1999a,b); (2) autoimmune or allergic diseases: inflammatory bowel diseases (Zhou and Mineshita, 1999), atopic dermatitis (Nose et al, 1999); (3) chemicals or trauma-induced diseases: hepatoxininduced hepatitis (Lin et al, 1996;Ohta et al, 1997Ohta et al, , 1998Ohta et al, , 2004, post-traumatic inflammatory aqueous flare elevation after smallincision cataract surgery (Ikeda et al, 2001;Nagaki et al, 2001), mucositis caused by anticancer drugs (Yuki et al, 2003) and (4) others: ischaemia/reperfusion injury-associated inflammatory process (Kabuto et al, 1997;Kondo et al, 2000;Xu et al, 2000;Hwang et al, 2002), and atherosclerosis. These studies experimentally demonstrated HLJDT as an effective anti-inflammatory agent.…”