Effects of osmotic pressure on prolactin and growth hormone secretion from organ-cultured eel pituitary

Suzuki, Reiko; Kaneko, Toyoji; Hirano, Tetsuya

doi:10.1007/bf00262877

Cited by 16 publications

(6 citation statements)

References 34 publications

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“…In Mozambique tilapia and Japanese eel, it has been reported that prolactin (PRL)-producing cells in the pituitary are sensitive to changes in osmotic pressure of the extracellular fluid. The PRL secretion from organ-cultured pituitaries increased when the medium osmolality was slightly reduced (Grau et al, 1981;Suzuki et al, 1991), and this osmosensitive PRL release was linked with volume changes in PRL cells (Weber et al, 2004). This process might also be mediated by AQP3, since we detected substantial expression of AQP3 in the tilapia pituitary.…”

Section: Discussionmentioning

confidence: 63%

Aquaporin-3 expressed in the basolateral membrane of gill chloride cells in Mozambique tilapia Oreochromis mossambicus adapted to freshwater and seawater

Watanabe

Kaneko

Aida

2005

Journal of Experimental Biology

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105

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We have cloned a homologue of mammalian aquaporin-3 (AQP3) from gills of Mozambique tilapia using a reverse transcription-polymerase chain reaction (RT-PCR). The deduced amino acid sequence shared 64-75% homology with other vertebrate AQP3 homologues. RT-PCR revealed that tilapia AQP3 was expressed in the brain, pituitary, kidney, spleen, intestine, skin, eye and gill in tilapia adapted to freshwater (FW) and seawater (SW). We also examined functional characteristics of tilapia AQP3 using Xenopus oocytes as an in vitro transcribed cRNA expression system. Osmotic water permeability (Pf) of Xenopus oocytes expressing tilapia AQP3 was about 30-fold higher than that of control oocytes, and was 80% inhibited by treatment with 0.3·mmol·l -1 HgCl 2 . Lightmicroscopic immunocytochemistry of branchial epithelia revealed that tilapia AQP3 was expressed in gill chloride cells of FW-and SW-adapted tilapia. Electronmicroscopic immunocytochemistry further demonstrated that tilapia AQP3 was localized in the basolateral membrane of gill chloride cells. Basolateral localization of AQP3 in gill chloride cells suggests that AQP3 is involved in regulatory volume changes and osmoreception, which could trigger functional differentiation of chloride cells.

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Section: Discussionmentioning

confidence: 63%

Aquaporin-3 expressed in the basolateral membrane of gill chloride cells in Mozambique tilapia Oreochromis mossambicus adapted to freshwater and seawater

Watanabe

Kaneko

Aida

2005

Journal of Experimental Biology

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105

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“…A similar pattern is also found in the European eel (Quérat et al 1994). In vitro studies also indicate that the secretion of PRL by organ-cultured teleost pituitaries is activated in a hypotonic medium (Ingleton et al 1973, Nagahama et al 1975, Benjamin & Baker 1976, Suzuki et al 1991b. Thus, the level of pituitary PRL is a possible candidate for monitoring the salinity preference of the wild eels during silvering.…”

Section: Introductionmentioning

confidence: 56%

“…The change of the PRL mRNA expression is sharper than the change of Sr:Ca ratios at the otolith edge in response to the salinity shift during eel silvering. The reason may be that the required response time of the PRL mRNA expression levels to (Suzuki & Hirano 1991, Suzuki et al 1991b). …”

Section: Discussionmentioning

confidence: 99%

Salinity preference of silvering Japanese eel Anguilla japonica: evidence from pituitary prolactin mRNA levels and otolith Sr:Ca ratios

Han¹,

Yu²,

Liao³

et al. 2003

Mar. Ecol. Prog. Ser.

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The salinity preference of Japanese eel Anguilla japonica during silvering was validated from the viewpoints of physiological ecology by examining the pituitary prolactin (PRL) mRNA expression and the otolith strontium:calcium (Sr:Ca) ratios of the eels of different sexual maturity stages collected in an estuary of Taiwan. The PRL possessed a putative signal peptide of 24 amino acids and a mature peptide of 185 amino acids. RT-PCR and real-time quantitative PCR analyses indicated that the transcript levels of PRL mRNA significantly decreased from yellow to silver stage. On the contrary, the Sr:Ca ratios at the otolith edge of silver eels, which recorded their latest experience of salinity history, significantly increased. The increase in otolith Sr:Ca ratios and the decrease in PRL mRNA expression are consistent with the timing of migration from freshwater to seawater during silvering of the eel.KEY WORDS: Japanese eel · Salinity preference · Otolith · Sr:Ca ratio · Prolactin · mRNA · SilveringResale or republication not permitted without written consent of the publisher

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“…Kültz, 2013. Minute changes in the extracellular osmolality, together with the presence of some autocrine modulator proteins like Prl177 and Prl188, directly triggered Prl production in the European eel, S. 351 aurata, and the tilapias (Suzuki et al 1991;Uchida et al 2004;Mohammed-Geba et al 2015;Yamaguchi et al 2016). Moreover, it is noteworthy to mention that both plasma Prl levels and pituitary prl expression patterns corresponded in their upregulation in response to hyposmotic salinity in different perciform species, like O. mossambicus and S. aurata (Riley et al 2003;Laiz-Carrión et al 2009;Vargas-Chacoff et al 2009).…”

Section: Regulation Of Hormonal Transcripts By Different Environmentamentioning

confidence: 98%

Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities

Mohammed-Geba

Astola

Suárez

et al. 2016

Fish Physiol Biochem

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Aquaculture industry in the Mediterranean region exhibits a growing interest for the Mediterranean meager Argyrosomus regius. Some preliminary works showed a good growth performance of the species in nearly isosmotic salinities. However, the patterns of alteration of prolactin (Prl) as well as growth hormone (Gh)/insulin growth factor-1 (Igf1) axis at the molecular level are not yet described in this species. Therefore, we cloned and sequenced partial cDNAs for pituitary prolactin (prl) and growth hormone (gh), hepatic insulin-like growth factor (igf1), and β-actin (actb). Expression patterns of these transcripts were tested in juveniles of A. regius acclimated to four different environmental salinities: (1) 5 ‰ (hyposmotic); (2) 12 ‰ (isosmotic); (3) 38 ‰ (hyperosmotic; seawater control); and (4) 55 ‰ (extremely hyperosmotic). All investigated transcripts shared high sequence identities with their counterparts in other perciformes. prl mRNA levels showed inverse pattern with increasing salinities. gh mRNA enhanced significantly in both 12 and 55 ‰ salinity groups in comparison with the control group, while igf1 showed its maximum expression levels under the nearly isosmotic environment. The results indicated clear sensitivity of prl, gh and igf1 to changes in environmental salinity, which can possibly control the euryhalinity capacity of this species.

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Effects of osmotic pressure on prolactin and growth hormone secretion from organ-cultured eel pituitary

Cited by 16 publications

References 34 publications

Aquaporin-3 expressed in the basolateral membrane of gill chloride cells in Mozambique tilapia Oreochromis mossambicus adapted to freshwater and seawater

Aquaporin-3 expressed in the basolateral membrane of gill chloride cells in Mozambique tilapia Oreochromis mossambicus adapted to freshwater and seawater

Salinity preference of silvering Japanese eel Anguilla japonica: evidence from pituitary prolactin mRNA levels and otolith Sr:Ca ratios

Molecular performance of Prl and Gh/Igf1 axis in the Mediterranean meager, Argyrosomus regius, acclimated to different rearing salinities

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