Effects of osmotic shock on production of beta-carotene and glycerol in a naturally isolated strain of Dunaliella salina

Montazeri‐Najafabady, Nima; Negahdaripour, Manica; Salehi, Mohammad Hossein; Morowvat, Mohammad Hossein; Shaker, Saeedeh; Ghasemi, Younes

doi:10.7324/japs.2016.60825

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…Water in algal biomasses leads to swelling of the centers of cell matrixes and acts as co-polar solvent that facilitates extraction (Du et al, 2016). Among wet extraction techniques (Yoo et al, 2012), osmotic shocks are applied with sudden changes in solute concentrations around cells following the addition of osmotic agents that cause rapid changes in water movement across semipermeable cell membranes (Montazeri-Najafabady et al, 2016). Under these conditions, pressure differences lead to removal of certain substances from cells.…”

Section: Introductionmentioning

confidence: 99%

Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

Hadiyanto

Adetya

2018

Food Sci Biotechnol

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In this study, we optimized the process for extracting lipids and proteins from wet biomasses of Spirulina sp. using a 4-kHz ultrasonic osmotic shock method with ultrasound enhancement at a constant frequency of 40 kHz. Optimization was conducted using a response surface methodology (RSM) at an osmotic NaCl concentration of 10-30%, solvent:biomass ratio of 5-15 v/ w, and extraction times of 20-50 min. The present osmotic shock method with ultrasound irradiation increased lipid yields to 6.65% in the presence of 11.9% NaCl, a solvent:biomass ratio of 12:1 v/w, and a 22-min extraction time, and protein yields to 43.96% with 15.12% NaCl, a solvent:biomass ratio of 10:1 v/w, and a 30-min extraction time.

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Section: Introductionmentioning

confidence: 99%

Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

Hadiyanto

Adetya

2018

Food Sci Biotechnol

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“…Although many studies have been done on Dunaliella Salina isolated from the Maharlu Salt Lake [39][40][41][42][43], its natural culture medium has not been yet used for cultivation of Dunaliella Salina. Based on the thorough investigation carried out herein, only less than 23 percent of cultivable land is required to produce biodiesel from microalgae to replace all diesel currently consumed in Iran.…”

Section: Introductionmentioning

confidence: 99%

The Biodiesel of Microalgae as a Solution for Diesel Demand in Iran

et al. 2018

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Among the fossil fuels, diesel has the major share in petroleum product consumption. Diesel demand in Iran has increasingly grown due to the low price of diesel, a high subsidy, and an unsuitable consumption pattern. During 2006-2007, this growth imposed 2.2 billion liters of imports, which were equivalent to 7.5% of diesel production in 2007 and cost about $1.2 billion. Therefore, the government implemented fuel rationing in 2007 and a targeted subsidy law in 2010. These projects have not gained effective control of consumption due to the wide gap between the international diesel price and the domestic price. Diesel import after the implementation of fuel rationing and the targeted subsidy law in 2011 imposed 3.6 billion liters of import and cost about $2.2 billion. Therefore, the government will need fundamental strategies and policies to face and control the negative impact on the economy and the environment. Third generation fuels, biofuels, as another supplementary approach seems to have the capability to reduce the petroleum requirement. This paper investigates the potential of biodiesel as diesel alternative fuel from oil seeds and microalgae in Iran along with evaluating the policy for reducing diesel consumption. Dunaliella salina as an indigenous green microalga isolated from the Maharlu Salt Lake was cultivated in an integration of an airlift system and a raceway pond (IARWP) to prove microalgal potentials in Iran. Additionally, the natural culture medium from the Maharlu Salt Lake was utilized for Dunaliella salina in order to commercialize and reduce cultivation cost. Compared to oilseeds, microalgae because of their high lipid content have much potential to solve a fuel consumption problem. This paper found that only 21 percent of cultivable land is needed to replace the diesel currently consumed in Iran with microalgal biodiesel.

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“…Effects of salt stress and cooling regimes on the pigment composition of Dunaliella salina… and stress proteins [1][2][3][4][5][6][7]. Accumulation usually occurs under conditions of stressful growth.…”

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confidence: 99%

Effects of salt stress and cooling regimes on the pigment composition of Dunaliella salina cells

Chernobai,

Kadnikova,

Vozovyk

et al. 2023

Biophysical Bulletin

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Background: Microalgae are able to produce a significant amount of biologically significant substances. In connection with the growing popularity of microalgae, it is important to develop effective methods for storing cultures and creating strain banks. This will not only meet the needs of science and biotechnology for viable and sustainable crops, but will also solve the problem of biodiversity conservation. Objectives: study the effect of salt stress and cooling regimes on the pigment composition of microalgae Dunaliella salina cells in order to increase their safety after freezing-thawing. Materials and methods: The objects of the study were the unicellular green microalgae D. salina. Cultivation was carried out according to the standard method on nutrient media with different amounts of NaCl and trace elements. Adaptation to low temperatures was carried out by exposure of samples in the dark at temperature 4°C for 24 hours. Freezing was performed by placing 1 ml of the cell suspension in a 1.8 ml polypropylene cryogenic vial (Nunc, Sigma-Aldrich), cooled at a rate of 1 deg/min using a Mr. Frosty with following regimes: to -10°С, -40°С, -40°С followed by immersion to liquid nitrogen or direct immersion to liquid nitrogen (-196°С). Thawing was carried out in a water bath (37°С) with continuous shaking for 1–2 min. Microscopic examinations were carried out on an LSM-510 Meta laser scanning microscope (Carl Zeiss, Germany) upon excitation by a diode laser with a wavelength of 405 nm and 573 nm using a Nile Red stain. Results: It has been established that the formation of intracellular lipid globules and the synthesis of carotenoids in D. salina cells contribute to an increase in the concentration and number of motile cells after freezing-thawing. It has been shown that during rapid cooling, adaptive mechanisms do not have time to turn on in cells, and complete destruction of carotene-containing lipid globules occurs. Conclusions: Cryopreservation of D. salina cells should be carried out at a rate of 1 deg/min to -40°C, followed by immersion in liquid nitrogen and a mandatory stage of precultivation at 4°C for 24 hours. This approach allows the cells to adapt to a decrease in temperature, which contributes to the best result after freezing-thawing.

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Effects of osmotic shock on production of beta-carotene and glycerol in a naturally isolated strain of Dunaliella salina

Cited by 7 publications

References 11 publications

Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

The Biodiesel of Microalgae as a Solution for Diesel Demand in Iran

Effects of salt stress and cooling regimes on the pigment composition of Dunaliella salina cells

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