Effects of Ovarian Hormones on Ovarian Capillary Blood Flow in Anoestrous Ewes

Brown, B. W.; Mattner, P. E.

doi:10.1071/bi9840389

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…Unlike the vasculature in other reproductive organs such as the uterus and the oviducts, the ovarian vasculature seems relatively insensitive to peripheral concentrations of oestradiol. This notion is supported not only by the absence of a concurrent increase in capillary blood flow in the contralateral non-ovulatory ovary at ovulation but also by the fact that the mean level of flow in such ovaries (see Table 2 (Brown & Mattner, 1984 …”

Section: Discussionsupporting

confidence: 48%

“…A study with anoestrous ewes on the effects of ovarian hormones on the ovarian vasculature (Brown & Mattner, 1984) showed that progesterone treatment before oestrogen produced a signifi¬ cant rise in ovarian stromal capillary blood flow while treatment with oestrogen alone or progester¬ one alone had no such effect. This, together with the finding that ovarian stromal blood flow normally increases at oestrus in cyclic ewes (Brown et ai, 1974), suggests that the rate of ovarian blood flow around ovulation may be a critical factor for normal ovarian function.…”

Section: Introductionmentioning

confidence: 99%

“…It is assumed that the weight of the ovarian stroma would change very little over the 7 days between blood flow measurement and tissue sampling (Brown & Mattner, 1984) and consequently, ovarian blood flow was also calculated on a unit weight basis (ml/min/100 g).…”

Section: Introductionmentioning

confidence: 99%

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Ovarian capillary blood flow in seasonally anoestrous ewes induced to ovulate by treatment with GnRH

Brown

Cognie²,

Chemineau³

et al. 1988

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Summary. The In the ewes exhibiting normal luteal function, the mean ovarian capillary blood flow was significantly greater (P < 0\m=.\01) than that for ewes having abnormal luteal function. Irrespective of the type of CL produced, capillary blood flow was significantly greater (P < 0\m=.\05) in ovulatory ovaries than in non-ovulatory ovaries.These findings indicate that the rate of capillary blood flow in ovaries near ovulation may be a critical factor in normal development and maturation of preovulatory follicles and function of subsequently formed CL.

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Section: Discussionsupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

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Ovarian capillary blood flow in seasonally anoestrous ewes induced to ovulate by treatment with GnRH

Brown

Cognie²,

Chemineau³

et al. 1988

Reproduction

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“…Post-operative analgesia and antibiotic cover was provided in the form of a non-steroidal anti-inflammatory drug (2.2 mg kg −1 ; Finadyne, Shering Plough Animal Health, Herts, UK) and broad spectrum antibiotic (3–4 ml animal −1 , Streptacare, Animal Care, Ltd, York, UK) for 3–5 days after surgery. Prior to aTP, each ewe was treated with a regime of progesterone and estradiol benzoate previously shown to increase ovarian and uterine blood flow (Brown and Mattner, 1984). This regime consisted of progestagen impregnated sponges (flugestone acetate; Chronogest CR, Intervet UK Ltd, Milton Keynes, UK) implanted intravaginally for 10–11 days, with sponge withdrawal and estradiol benzoate injection (100 µg animal −1 ) on the day before aTP.…”

Section: Methodsmentioning

confidence: 99%

Restoration of ovarian function and natural fertility following the cryopreservation and autotransplantation of whole adult sheep ovaries

et al. 2014

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STUDY QUESTIONIs it possible to restore ovarian function and natural fertility following the cryopreservation and autotransplantation of whole ovaries, complete with vascular pedicle, in adult females from a large monovulatory animal model species (i.e. sheep)?SUMMARY ANSWERFull (100%) restoration of acute ovarian function and high rates of natural fertility (pregnancy rate 64%; live birth rate 29%), with multiple live births, were obtained following whole ovary cryopreservation and autotransplantation (WOCP&TP) of adult sheep ovaries utilizing optimized cryopreservation and post-operative anti-coagulant regimes.WHAT IS KNOWN ALREADYFertility preservation by WOCP&TP requires successful cryopreservation of both the ovary and its vascular supply. Previous work has indicated detrimental effects of WOCP&TP on the ovarian follicle population. Recent experiments suggest that these deleterious effects can be attributed to an acute loss of vascular patency due to clot formation induced by damage to ovarian arterial endothelial cells.STUDY DESIGN, SIZE, DURATIONStudy 1 (2010–2011; N = 16) examined the effect of post-thaw perfusion of survival factors (angiogenic, antioxidant, anti-apoptotic; n = 7–8) and treatment with aspirin (pre-operative versus pre- and post-operative (n = 7–9)) on the restoration of ovarian function for 3 months after WOCP&TP. Study 2 (2011–2012; N = 16) examined the effect of cryoprotectant (CPA) perfusion time (10 versus 60 min; n = 16) and pre- and post-operative treatment with aspirin in combination with enoxaparine (Clexane®; n = 8) or eptifibatide (Integrilin®; n = 8) on ovarian function and fertility 11–23 months after WOCP&TP.PARTICIPANTS/MATERIALS, SETTING, METHODSBoth studies utilized mature, parous, Greyface ewes aged 3–6 years and weighing 50–75 kg. Restoration of ovarian function was monitored by bi-weekly blood sampling and display of behavioural oestrus. Blood samples were assayed for gonadotrophins, progesterone, anti-Müllerian Hormone and inhibin A. Fertility restoration in Study 2 was quantified by pregnancy rate after a 3 month fertile mating period and was confirmed by ultrasound, hormonal monitoring and live birth. Ovarian function was assessed at sacrifice by ovarian appearance and vascular patency (Doppler ultrasound) and by follicular histology.MAIN RESULTS AND THE ROLE OF CHANCEIn Study 1, survival factors were found to have no benefit, but the inclusion of pre-operative aspirin resulted in four ewes showing acute restoration of ovarian function within 3 weeks and a further six ewes showing partial restoration. The addition of post-operative aspirin alone had no clear benefit. In Study 2, combination of aspirin with additional post-operative anti-coagulants resulted in total acute restoration of ovarian function in 14/14 ewes within 3 weeks of WOCP&TP, with 9/14 ewes becoming pregnant and 4/14 giving birth to a total of seven normal lambs. There was no difference between anti-coagulants in terms of restoration of reproductive function and fertility. In contrast, the duration ...

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“…The sponges were removed immediately after the cannulation procedure, 36-40 h before blood flow measurement. Pre-treatment of ewes with progesterone only, has no effect on ovarian capillary blood flow (Brown & Mattner, 1984).…”

Section: Methodsmentioning

confidence: 99%

Blood flow in the ovaries and ovarian follicles of Romanov and Prealpes-du-Sud ewes

Brown

Driancourt²

1989

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Radioactive microspheres (15 microns diameter) were used to measure capillary blood flow rates in the ovaries and ovarian follicles (Qf) in high fecund Romanov and low fecund Préalpes-du-Sud ewes at the preovulatory stage of the oestrous cycle. Additionally, assessments of the percentage of arterial blood passing through ovarian arterio-venous anastomoses were obtained. The mean +/- s.e.m. Qf per unit volume of theca [ml/min) x 10(4)/mm3) for non-atretic follicles in Romanov ewes was significantly greater (P less than 0.05) than that in Préalpes ewes (365.8 +/- 42.4, n = 19, compared with 241.3 +/- 30.1, n = 14). For each breed, the mean Qf value for non-atretic follicles was 8-10 times greater than that for atretic follicles. In Romanov ewes, total Qf [ml/min) x 10(4) and Qf per unit volume of theca was greatest in small-sized follicles (3.1-5.0 mm) while in Préalpes ewes, maximum flow was attained in larger-sized follicles (5.1-7.0 mm). The elevated Qf in small-sized follicles in Romanov ewes may be conducive to more follicles achieving maturation at a smaller diameter in this breed than occurs in the Préalpes ewes. The absence of flow through ovarian arterio-venous anastomoses in the Romanov, but not in the Préalpes, ewes suggests different mechanisms for controlling the distribution of the total ovarian blood supply in the 2 breeds.

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Effects of Ovarian Hormones on Ovarian Capillary Blood Flow in Anoestrous Ewes

Cited by 8 publications

References 16 publications

Ovarian capillary blood flow in seasonally anoestrous ewes induced to ovulate by treatment with GnRH

Ovarian capillary blood flow in seasonally anoestrous ewes induced to ovulate by treatment with GnRH

Restoration of ovarian function and natural fertility following the cryopreservation and autotransplantation of whole adult sheep ovaries

Blood flow in the ovaries and ovarian follicles of Romanov and Prealpes-du-Sud ewes

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