Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Liang, Feiyan; Lindblad, Peter

doi:10.1016/j.ymben.2016.06.005

Cited by 91 publications

(61 citation statements)

References 46 publications

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“…Enzyme and metabolite pools of the regenerative phase of the CB cycle are proposed to play a role in determining the overall carbon fixation rate1920. Modifications of other steps in the CB cycle, such as those catalysed by sedoheptulose 1,7-bisphosphatase and transketolase, have been utilized to improve carbon fixation192021. Therefore, we hypothesize that the supplementary carbon source glucose would increase availability of the substrate for carbon fixation, D -ribulose-1,5-bisphosphate (R15P), and enhance efficiency of the reaction catalysed by RuBisCO.…”

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confidence: 99%

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

2017

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Cyanobacteria have attracted much attention as hosts to recycle CO2 into valuable chemicals. Although cyanobacteria have been engineered to produce various compounds, production efficiencies are too low for commercialization. Here we engineer the carbon metabolism of Synechococcus elongatus PCC 7942 to improve glucose utilization, enhance CO2 fixation and increase chemical production. We introduce modifications in glycolytic pathways and the Calvin Benson cycle to increase carbon flux and redirect it towards carbon fixation. The engineered strain efficiently uses both CO2 and glucose, and produces 12.6 g l−1 of 2,3-butanediol with a rate of 1.1 g l−1 d−1 under continuous light conditions. Removal of native regulation enables carbon fixation and 2,3-butanediol production in the absence of light. This represents a significant step towards industrial viability and an excellent example of carbon metabolism plasticity.

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confidence: 99%

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

2017

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“…The best results have been obtained by enhancing the homogeneity of light distribution within photobioreactors [82] and by decreasing their susceptibility to photodamage [89]. Besides the obvious strategy of targeting antenna systems, photoprotection was also enhanced by increasing photochemical quenching by boosting CO 2 fixation through over-expressing RuBisCO activity, and other rate-liming enzymes in the CBB cycle [109].…”

Section: Discussionmentioning

confidence: 99%

“…Besides RuBisCO, other CBBc enzymes and accessory proteins have been targeted, e.g., sedoheptulose 1,7-bisphosphatase from C. reinhardtii has been successfully over-expressed in D. bardawil, resulting in a significant enhancement of photosynthetic efficiency ( Figure 5) [108]. Over-expression of the fructose 1,6-bisphosphatase in Synechocystis enhanced the growth rate with respect to the control genotype under EL conditions ( Figure 5) [109]. A strong raise in the photosynthetic productivity of Synechocystis was obtained by over-expressing RuBisCO, sedoheptulose1,7-biphosphatase, fructose-bisphosphate aldolase and trans-ketolase [110].…”

Section: Rubisco As Target To Improve Carbon Assimilation Efficiencymentioning

confidence: 99%

Potential and Challenges of Improving Photosynthesis in Algae

Vecchi

Barera

Bassi

et al. 2020

Plants

108

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Sunlight energy largely exceeds the energy required by anthropic activities, and therefore its exploitation represents a major target in the field of renewable energies. The interest in the mass cultivation of green microalgae has grown in the last decades, as algal biomass could be employed to cover a significant portion of global energy demand. Advantages of microalgal vs. plant biomass production include higher light-use efficiency, efficient carbon capture and the valorization of marginal lands and wastewaters. Realization of this potential requires a decrease of the current production costs, which can be obtained by increasing the productivity of the most common industrial strains, by the identification of factors limiting biomass yield, and by removing bottlenecks, namely through domestication strategies aimed to fill the gap between the theoretical and real productivity of algal cultures. In particular, the light-to-biomass conversion efficiency represents one of the major constraints for achieving a significant improvement of algal cell lines. This review outlines the molecular events of photosynthesis, which regulate the conversion of light into biomass, and discusses how these can be targeted to enhance productivity through mutagenesis, strain selection or genetic engineering. This review highlights the most recent results in the manipulation of the fundamental mechanisms of algal photosynthesis, which revealed that a significant yield enhancement is feasible. Moreover, metabolic engineering of microalgae, focused upon the development of renewable fuel biorefineries, has also drawn attention and resulted in efforts for enhancing productivity of oil or isoprenoids.

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“…Balancing the appropriate enzyme activity in the central carbon metabolism enzymes that are connected with the CBB by, for example, overexpression of one of four separate enzymes (fructose bisphosphate aldolase, sedoheptulose bisphosphatase, RuBisCO, or transketolase) favored an increase in the carbon binding capacity in S. sp. PCC 6803 [ 21 ]. Modification of the expression of these CBB-related enzymes increased the rate of heterologous ethanol production by S. sp.…”

Section: Photosynthesis: Generating Energy For Biopolymer Synthesimentioning

confidence: 99%

Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms

Ciebiada

Kubiak

Daroch

2020

IJMS

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Cyanobacteria are photoautotrophic bacteria commonly found in the natural environment. Due to the ecological benefits associated with the assimilation of carbon dioxide from the atmosphere and utilization of light energy, they are attractive hosts in a growing number of biotechnological processes. Biopolymer production is arguably one of the most critical areas where the transition from fossil-derived chemistry to renewable chemistry is needed. Cyanobacteria can produce several polymeric compounds with high applicability such as glycogen, polyhydroxyalkanoates, or extracellular polymeric substances. These important biopolymers are synthesized using precursors derived from central carbon metabolism, including the tricarboxylic acid cycle. Due to their unique metabolic properties, i.e., light harvesting and carbon fixation, the molecular and genetic aspects of polymer biosynthesis and their relationship with central carbon metabolism are somehow different from those found in heterotrophic microorganisms. A greater understanding of the processes involved in cyanobacterial metabolism is still required to produce these molecules more efficiently. This review presents the current state of the art in the engineering of cyanobacterial metabolism for the efficient production of these biopolymers.

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Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

Cited by 91 publications

References 46 publications

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

Potential and Challenges of Improving Photosynthesis in Algae

Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms

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