Effects of Oxfendazole on Some Biochemical Parameters in Hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) Larvae Reared on Artificial Diet

Abstract

Oxfendazole is used as an antihelminthic drug in parasite infections of animals. Oxfendazole was added at different concentrations (0, 0.0015, 0.015, 0.15, and 1.5%) to artificial diet and the wax moth Galleria mellonella larvae were reared to 7th stage. The changes in metabolic enzymes activity in G. mellonella hemolymph tissue were investigated. It was found that the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was increased which are cell damage biomarkers. The highest concentration of this antihelmintic was increased the enzme activity (ALT) from 329 ± 4.33 to 529. 5 ± 3.17 U/L. Gamma glutamyl transferase (GGT) enzyme activity was increased from 0.87 ± 0.1 to 4.32 ± 0.22 U/L. Creatine kinase (CK) and amylase (AMYL) activities were significantly increased in hemolymph of G. mellonella larvae fed with the highest oxfendazole concentration (1.5%) when compared to the control group. Our results indicate that oxfendazole can be used as an insecticide with a well-adjusted concentrations because of its low acute toxicity to humans and other non-target organisms.