Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Parra-Ortiz, Elisa; Browning, Kathryn L.; Damgaard, Liv Sofia Elinor; Nordström, Randi; Micciulla, Samantha; Bucciarelli, Saskia; Malmsten, Martin

doi:10.1016/j.jcis.2018.12.007

Cited by 29 publications

(35 citation statements)

References 78 publications

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“…For instance, the oxidation of polyunsaturated lipids can destabilize lipid membranes, leading to the formation of pores and the subsequent breakdown of the membrane. 38 …”

Section: Introductionmentioning

confidence: 99%

Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes

et al. 2020

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Cholesterol renders mammalian cell membranes more compact by reducing the amount of voids in the membrane structure. Because of this, cholesterol is known to regulate the ability of cell membranes to prevent the permeation of water and water-soluble molecules through the membranes. Meanwhile, it is also known that even seemingly tiny modifications in the chemical structure of cholesterol can lead to notable changes in membrane properties. The question is, how significantly do these small changes in cholesterol structure affect the permeability barrier function of cell membranes? In this work, we applied fluorescence methods as well as atomistic molecular dynamics simulations to characterize changes in lipid membrane permeability induced by cholesterol oxidation. The studied 7β-hydroxycholesterol (7β-OH-chol) and 27-hydroxycholesterol (27-OH-chol) represent two distinct groups of oxysterols, namely, ring- and tail-oxidized cholesterols, respectively. Our previous research showed that the oxidation of the cholesterol tail has only a marginal effect on the structure of a lipid bilayer; however, oxidation was found to disturb membrane dynamics by introducing a mechanism that allows sterol molecules to move rapidly back and forth across the membrane—bobbing. Herein, we show that bobbing of 27-OH-chol accelerates fluorescence quenching of NBD-lipid probes in the inner leaflet of liposomes by dithionite added to the liposomal suspension. Systematic experiments using fluorescence quenching spectroscopy and microscopy led to the conclusion that the presence of 27-OH-chol increases membrane permeability to the dithionite anion. Atomistic molecular dynamics simulations demonstrated that 27-OH-chol also facilitates water transport across the membrane. The results support the view that oxysterol bobbing gives rise to successive perturbations to the hydrophobic core of the membrane, and these perturbations promote the permeation of water and small water-soluble molecules through a lipid bilayer. The observed impairment of permeability can have important consequences for eukaryotic organisms. The effects described for 27-OH-chol were not observed for 7β-OH-chol which represents ring-oxidized sterols.

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“…For instance, the oxidation of polyunsaturated lipids can destabilize lipid membranes, leading to the formation of pores and the subsequent breakdown of the membrane. 38 …”

Section: Introductionmentioning

confidence: 99%

Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes

et al. 2020

View full text Add to dashboard Cite

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“…Moreover, Complexes I and III of the electron transport chain are the main source of ROS production in mitochondria [ 12 ]. Parra-Ortiz et al [ 13 ] stated that oxidative stress instigates several modifications in lipids that generate oxidized- specific products (e.g., oxidized low-density lipoprotein or cholesteryl-esters that stimulate macrophages via toll-like receptor-4 (TLR4) and spleen tyrosine kinase) that excite inflammation and induce immune responses [ 14 ]. Further, generation of ROS in adipocytes perpetuates chronic inflammation and stimulates pro-inflammatory adipokines in the target tissue [ 13 ].…”

Section: Inflammation and Oxidative Stressmentioning

confidence: 99%

“…Parra-Ortiz et al [ 13 ] stated that oxidative stress instigates several modifications in lipids that generate oxidized- specific products (e.g., oxidized low-density lipoprotein or cholesteryl-esters that stimulate macrophages via toll-like receptor-4 (TLR4) and spleen tyrosine kinase) that excite inflammation and induce immune responses [ 14 ]. Further, generation of ROS in adipocytes perpetuates chronic inflammation and stimulates pro-inflammatory adipokines in the target tissue [ 13 ]. Additionally, modulation of macrophages activities due to the bioenergetics and metabolic alteration increase phospholipid oxidation in tissues that leads to the modification of membrane properties and stimulate inflammation [ 15 ].…”

Section: Inflammation and Oxidative Stressmentioning

confidence: 99%

The Potential Role of Polyphenols in Oxidative Stress and Inflammation Induced by Gut Microbiota in Alzheimer’s Disease

et al. 2021

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Gut microbiota (GM) play a role in the metabolic health, gut eubiosis, nutrition, and physiology of humans. They are also involved in the regulation of inflammation, oxidative stress, immune responses, central and peripheral neurotransmission. Aging and unhealthy dietary patterns, along with oxidative and inflammatory responses due to gut dysbiosis, can lead to the pathogenesis of neurodegenerative diseases, especially Alzheimer’s disease (AD). Although the exact mechanism between AD and GM dysbiosis is still unknown, recent studies claim that secretions from the gut can enhance hallmarks of AD by disturbing the intestinal permeability and blood–brain barrier via the microbiota–gut–brain axis. Dietary polyphenols are the secondary metabolites of plants that possess anti-oxidative and anti-inflammatory properties and can ameliorate gut dysbiosis by enhancing the abundance of beneficial bacteria. Thus, modulation of gut by polyphenols can prevent and treat AD and other neurodegenerative diseases. This review summarizes the role of oxidative stress, inflammation, and GM in AD. Further, it provides an overview on the ability of polyphenols to modulate gut dysbiosis, oxidative stress, and inflammation against AD.

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“…The cumulative result here is that the physiological functions of the cell membrane are altered, which can lead to cell damage, and even to cell death. [69][70][71] It has been reported that electroporation induces lipid peroxidation in bacteria, plant cells, and mammalian cells, as well as in liposomes made from polyunsaturated phospholipids. 1 The origins of ROS are diverse.…”

Section: Resealingmentioning

confidence: 99%

The Good and the Bad of Cell Membrane Electroporation

Balantič

Miklavčič

Križaj

et al. 2021

ACSi

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Electroporation is used to increase the permeability of the cell membrane through high-voltage electric pulses. Nowadays, it is widely used in different areas, such as medicine, biotechnology, and the food industry. Electroporation induces the formation of hydrophilic pores in the lipid bilayer of cell membranes, to allow the entry or exit of molecules that cannot otherwise cross this hydrophobic barrier. In this article, we critically review the basic principles of electroporation, along with the advantages and drawbacks of this method. We discuss the effects of electroporation on the key components of biological membranes, as well as the main applications of this procedure in medicine, such as electrochemotherapy, gene electrotransfer, and tissue ablation. Finally, we define the most relevant challenges of this romising area of research.

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Effects of oxidation on the physicochemical properties of polyunsaturated lipid membranes

Cited by 29 publications

References 78 publications

Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes

Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes

The Potential Role of Polyphenols in Oxidative Stress and Inflammation Induced by Gut Microbiota in Alzheimer’s Disease

The Good and the Bad of Cell Membrane Electroporation

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