Effects of oxidative stress and antioxidant treatments on eicosanoid synthesis and lipid peroxidation in long term human umbilical vein endothelial cells culture

Estrada-Garcı́a, L; Carrera-Rotllan, J; Puig-Parellada, P.

doi:10.1016/s0090-6980(01)00167-8

Cited by 15 publications

(9 citation statements)

References 24 publications

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“…In contrast, Belcher et al (1993) reported that the cytotoxic effect of human ox-LDL on porcine aortic endothelial cells in vitro was abolished by 2 weeks' supplementation of the LDL donors with the same dose of vitamin E as that used in the present study. Our results on vascular endothelial cell culture are contradictory to the present hypothesis postulating that antioxidants reduce cell damage (Estrada-García et al, 2002). In a study by Weinberg et al (2001), in accord to our study, smokers consumed a high polyunsaturated fat diet.…”

Section: After Vitamin E Supplementationcontrasting

confidence: 99%

Effects of vitamin E on the toxicity of oxidized LDL on endothelial cells in vitro in smokers vs nonsmokers on diets rich in fish

et al. 2005

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Objective: To clarify whether supplementation of vitamin E can alter the low density lipoprotein (LDL) oxidation properties and thereby affect endothelial cell function and prostacyclin production in smokers compared to nonsmokers on diets rich in fish in a pilot study. Design: The LDL of six smokers and six nonsmokers on habitual high fish diet was isolated before and after an 8-week supplementation of vitamin E (800 IU/day). LDL was oxidized by incubation with CuSO 4 . Cytotoxicity of LDL oxidized to different degrees on endothelial cells was investigated in vitro in these two groups. Setting: Helsinki University Central Hospital; Institute of Biomedicine, Pharmacology, University of Helsinki. Results: At baseline, the rate of oxidation was higher in nonsmokers than in smokers. The lag phase increased significantly after the supplementation of vitamin E both in smokers and nonsmokers. Native LDL dose dependently tended to reduce the viability of endothelial cells in vitro more markedly when isolated from smokers than from nonsmokers. Vitamin E supplementation had no beneficial effect on the cytotoxicity of oxidized LDLs in endothelial cell culture. On the other hand, simultaneous administration of Trolox s , the water-soluble analogue of vitamin E, attenuated the LDL cytotoxicity on endothelial cells. The vitamin E supplementation to LDL donors attenuated the increase in prostacyclin production both in smokers and nonsmokers. Conclusion: Supplementation of LDL donors (healthy male volunteers on habitual fish diet) with vitamin E increased the lag phase of LDL oxidation, but, on the other hand, did not influence in vitro cytotoxicity of LDL, or prostacyclin production.

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Section: After Vitamin E Supplementationcontrasting

confidence: 99%

Effects of vitamin E on the toxicity of oxidized LDL on endothelial cells in vitro in smokers vs nonsmokers on diets rich in fish

et al. 2005

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“…Similarly to the cell proliferation assay, the choice of trypan blue dye exclusion test to evaluate cell viability was based on its greater simplicity. This method was also recently used by other authors to evaluate cellular viability (18,21). The study design attempted to simulate the clinical situation of using the calcium phosphate cements by allowing both a direct contact of the cells with the tested materials and also by verifying if degradation and/or dissolution products of these would affect cell viability and proliferation.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Two Novel Formulations of Calcium Phosphate Cements: A Comparative In Vitro Study

et al. 2005

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The purpose was to evaluate the cytotoxicity of two novel formulations (alpha and beta) of calcium phosphate cements. Positive control, represented by a commercial hydroxyapatite cement, and negative control were included for comparative purposes. A continuous lineage of fibroblastic cells was used, and the effect of the tested materials on both cell proliferation and viability was assessed by counting cell number on hemocytometer and by the trypan blue exclusion test, respectively. Study design attempted to simulate clinical use by allowing direct and indirect contact of cells and cements. Results were analyzed by the Kruskal-Wallis test and indicated that the beta formulation was extremely cytotoxic (P < 0.001), because this material induced the greatest reduction on cell proliferation and viability. The alpha formulation behaved similarly to the positive control regarding its effect on cell proliferation and viability. Thus, it is concluded that alpha formulation has promise for further evaluation of its behavior in vivo.

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“…Another important interaction between iron and fatty acids is the implication of the iron‐containing enzyme catalase in the protection against peroxidation. Since both AA and DHA are highly susceptible to peroxidation, an adequate intake of iron may reduce cell damage due to oxidative stress 36.…”

Section: Introductionmentioning

confidence: 99%

Does inadequate maternal iron or DHA status have a negative impact on an infant's functional outcomes?

2006

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Marginal intake of iron and ω‐3 long‐chain fatty acids (DHA) is prevalent among pregnant women. It is not clear to what extent poor iron or DHA status during pregnancy impacts on an infant's functional outcomes. A few studies suggest that inadequate maternal iron or DHA status may be associated with suboptimal functional outcomes in infants. In addition, there is a lack of prospective studies using randomized, double‐blind design or experimental studies with appropriate animal models. Although both nutrients are involved in early brain development and their metabolism is interrelated, no study has examined the interaction between iron and ω‐3 fatty acids during pregnancy. Conclusion: Long‐term studies on large cohorts of pregnant women and their infants are needed to determine whether inadequate iron or DHA status during pregnancy is detrimental to infant neurodevelopment.

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Effects of oxidative stress and antioxidant treatments on eicosanoid synthesis and lipid peroxidation in long term human umbilical vein endothelial cells culture

Cited by 15 publications

References 24 publications

Effects of vitamin E on the toxicity of oxidized LDL on endothelial cells in vitro in smokers vs nonsmokers on diets rich in fish

Effects of vitamin E on the toxicity of oxidized LDL on endothelial cells in vitro in smokers vs nonsmokers on diets rich in fish

Cytotoxicity of Two Novel Formulations of Calcium Phosphate Cements: A Comparative In Vitro Study

Does inadequate maternal iron or DHA status have a negative impact on an infant's functional outcomes?

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