Effects of Oxidized Cooking Oil and α-Lipoic Acid on Blood Antioxidants: Enzyme Activities and Lipid Peroxidation in Rats Fed a High-Fat Diet

Zalejska–Fiolka, Jolanta; Wielkoszyński, Tomasz; Kasperczyk, Sławomir; Kasperczyk, Aleksandra; Birkner, Ewa

doi:10.1007/s12011-011-9186-7

Cited by 10 publications

(12 citation statements)

References 20 publications

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“…On the other hand, these studies revealed a temporary increase in homocystein and 7-ketocholesterol concentrations in the groups of animals receiving oxidised oil. We also observed that addition of oxidised oil resulted in increased production of oxygen radicals, evidenced by elevated plasma MDA production in rats (17). We concluded that such diet causes the development of atherosclerotic changes.…”

Section: Discussionmentioning

confidence: 50%

Influence of Non-Oxidised and Oxidised Rapeseed Oil Consumption on Liver Metabolism Pathways and Non-Alcoholic Steatohepatitis Development in Rabbits

Zalejska–Fiolka¹,

Kasperczyk²,

Kasperczyk³

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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For 24 weeks, rabbits were fed feed containing non-oxidised or oxidised rapeseed oil. At the beginning of the experiment and every six weeks the rabbits were weighed and blood was taken. After the experiment was completed, their liver was dissected for biochemical and histological examinations. The activity of alanine aminotransferase, aspartate aminotrasferase, glutamate dehydrogenase, sorbitol dehydrogenase, and aldolase in blood plasma and liver were determined. Enzymes of the protein and liver metabolic pathways were determined using kinetic and spectrophotometric methods. The content of fatty acids was determined by means of fatty acid methyl ester concentration measurement using gas chromatography. It was found that the applied diet with oxidised rapeseed oil caused the development of slight liver steatosis and disturbances in the activity of enzymes involved in the liver pathways, despite the fact that it was a balanced diet, and differed only in the ratio of saturated to unsaturated fatty acids. The obtained results indicate that more profound oil oxidation and its increased supply in diet may result in the development of liver steatosis.

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Section: Discussionmentioning

confidence: 50%

Influence of Non-Oxidised and Oxidised Rapeseed Oil Consumption on Liver Metabolism Pathways and Non-Alcoholic Steatohepatitis Development in Rabbits

Zalejska–Fiolka¹,

Kasperczyk²,

Kasperczyk³

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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“…Indeed, the supplement of α-LA can protect against oxidative stress induced either by certain drugs or under various physiological and pathophysiological conditions [38,39]. In addition, previous studies undertaken in our lab have shown that supplementation of α-LA into AFs free-diets could enhance the antioxidant capability of broilers [40].…”

Section: Discussionmentioning

confidence: 99%

Effects of Lipoic Acid on Immune Function, the Antioxidant Defense System, and Inflammation-Related Genes Expression of Broiler Chickens Fed Aflatoxin Contaminated Diets

Zhao

et al. 2014

IJMS

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This study was designed to evaluate the effect of low level of Aflatoxin B1 (AFB1) on oxidative stress, immune reaction and inflammation response and the possible ameliorating effects of dietary alpha-lipoic acid (α-LA) in broilers. Birds were randomly allocated into three groups and assigned to receive different diets: basal diet, diet containing 74 μg/kg AFB1, and 300 mg/kg α-LA supplementation in diet containing 74 μg/kg AFB1 for three weeks. The results showed that the serum levels of malondialdehyde, tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ) in the AFB1-treated group were significantly increased than the control group. In addition, the increased expressions of interleukin 6 (IL6), TNFα and IFNγ were observed in birds exposed to the AFB1-contaminated diet. These degenerative changes were inhibited by α-LA-supplement. The activities of total superoxide dismutase and glutathione peroxidase, the levels of humoral immunity, and the expressions of nuclear factor-κB p65 and heme oxygenase-1, however, were not affected by AFB1. The results suggest that α-LA alleviates AFB1 induced oxidative stress and immune changes and modulates the inflammatory response at least partly through changes in the expression of proinflammatory cytokines of spleen such as IL6 and TNFα in broiler chickens.

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“…Our previous studies also showed that α-LA could enhance the activities of several enzymatic antioxidants, such as SOD and GSH-P X and increase de novo synthesis of GSH (Chen et al, 2011). Several studies have reported that α-LA can protect against hepatic oxidative stress, induced either by certain drugs or under various physiological and pathophysiological conditions (Abdel-Zaher et al, 2008; Zalejska-Fiolka et al, 2012). In view of these results, the effect of α-LA on AFB 1 -induced liver oxidative stress was evaluated here.…”

Section: Discussionmentioning

confidence: 99%

Protective Efficacy of Alpha-lipoic Acid against AflatoxinB1-induced Oxidative Damage in the Liver

Li¹,

G.²,

Zhao

et al. 2014

Asian Australas. J. Anim. Sci

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Alpha-lipoic acid (α-LA) is not only involved in energy metabolism, but is also a powerful antioxidant that can protect against hepatic oxidative stress induced by some drugs, toxins, or under various physiological and pathophysiological conditions. Here, we investigated the effect of α-LA against liver oxidative damage in broilers exposed to aflatoxin B1 (AFB1). Birds were randomly divided into four groups and assigned different diets: basal diet, 300 mg/kg α-LA supplementation in basal diet, diet containing 74 μg/kg AFB1, and 300 mg/kg α-LA supplementation in diet containing 74 μg/kg AFB1, for 3 weeks. The results revealed that the addition of 300 mg/kg α-LA protected against the liver function damage of broilers induced by chronic low dose of AFB1 as estimated by a significant (p<0.05) change in levels of plasma total protein, albumin, alkaline phosphatase and the activities of liver glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase. The histopathological analysis also showed that liver tissues were injured in the AFB1 diet, but this effect was alleviated by the addition of 300 mg/kg α-LA. Additionally, AFB1 induced a profound elevation of oxidative stress in birds, as indicated by an increase in malondialdehyde level, a decrease in glutathione peroxidase activity and a depletion of the glutathione content in the liver. All of these negative effects were inhibited by treatment with α-LA. Our results suggest that the inhibition of AFB1-induced excess production of lipid peroxides and the maintenance of intracellular antioxidant status may play important roles in the protective effects of α-LA against AFB1-induced oxidative damage in the liver.

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Effects of Oxidized Cooking Oil and α-Lipoic Acid on Blood Antioxidants: Enzyme Activities and Lipid Peroxidation in Rats Fed a High-Fat Diet

Cited by 10 publications

References 20 publications

Influence of Non-Oxidised and Oxidised Rapeseed Oil Consumption on Liver Metabolism Pathways and Non-Alcoholic Steatohepatitis Development in Rabbits

Influence of Non-Oxidised and Oxidised Rapeseed Oil Consumption on Liver Metabolism Pathways and Non-Alcoholic Steatohepatitis Development in Rabbits

Effects of Lipoic Acid on Immune Function, the Antioxidant Defense System, and Inflammation-Related Genes Expression of Broiler Chickens Fed Aflatoxin Contaminated Diets

Protective Efficacy of Alpha-lipoic Acid against AflatoxinB1-induced Oxidative Damage in the Liver

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