Effects of oxygen on Shewanella decolorationis NTOU1 electron transfer to carbon-felt electrodes

Li, Shiue-Lin; Freguia, Stefano; Liu, Shiu‐Mei; Cheng, Sheng-Shung; Tsujimura, Seiya; Shirai, Osamu; Kano, Kenji

doi:10.1016/j.bios.2010.04.038

Cited by 31 publications

(18 citation statements)

References 42 publications

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“…2A, the differential pulse (DP) voltammograms obtained for the monolayer biofilm contained two anodic peaks at redox potentials (E p ) of +50 mV and − 145 mV. Although the former peak has been previously assigned to the OmcA-MtrCAB complex (17), the latter peak is not attributable to the well-characterized redox species of strain MR-1, such as quinones, riboflavin, or FMN (19,20). When FMN was added to the reactor, the peak current at −145 mV increased in proportion to the concentration of supplemented FMN ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquinones

Okamoto

Hashimoto

Nealson

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

441

363

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Extracellular redox-active compounds, flavins and other quinones, have been hypothesized to play a major role in the delivery of electrons from cellular metabolic systems to extracellular insoluble substrates by a diffusion-based shuttling two-electron-transfer mechanism. Here we show that flavin molecules secreted by Shewanella oneidensis MR-1 enhance the ability of its outer-membrane c-type cytochromes (OM c-Cyts) to transport electrons as redox cofactors, but not free-form flavins. Whole-cell differential pulse voltammetry revealed that the redox potential of flavin was reversibly shifted more than 100 mV in a positive direction, in good agreement with increasing microbial current generation. Importantly, this flavin/OM c-Cyts interaction was found to facilitate a one-electron redox reaction via a semiquinone, resulting in a 10 3 -to 10 5 -fold faster reaction rate than that of free flavin. These results are not consistent with previously proposed redox-shuttling mechanisms but suggest that the flavin/OM c-Cyts interaction regulates the extent of extracellular electron transport coupled with intracellular metabolic activity.iron-reducing bacteria | electromicrobiology | microbial fuel cell | whole-cell voltammetry | flavin mononucleotide

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Section: Resultsmentioning

confidence: 99%

Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquinones

Okamoto

Hashimoto

Nealson

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

441

363

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“…In addition to OM c-Cyts, MR-1 cells are known to synthesize several flavin and quinone derivatives that function as electron shuttles, a few of which strongly bind to the outer cell surface and provide redox signals characteristic of immobilized electroactive species (Saffarini et al, 2002;Okamoto et al, 2009;Bouhenni et al, 2010). Therefore, the large difference in E m between purified OM c-Cyts and intact cells has led many researchers to speculate that the observed redox wave at 50 mV is attributable to cell-attached menaquinone, whose midpoint potential is approximately 80 mV (Li et al, 2010;Zhao et al, 2010), rather than OM c-Cyts.…”

Section: Whole-microorganism Voltammetrymentioning

confidence: 99%

Spectroelectrochemical Investigation on Biological Electron Transfer Associated with Anode Performance in Microbial Fuel Cells

Okamoto¹,

Hashimoto²,

Nakamura³

2012

Recent Trend in Electrochemical Science and Technology

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“…The continuous air bubbling in the anode chamber probably helped with the growth of aerobic microbes due to a direct reduction of oxygen in the cell to increase biomass production. Although increase in biomass means increase in the NADH production, which leads to increase in electrons production, most of these electrons however will be consumed for biomass before able to generate electricity [3]. This could be the reason for the high R int observed from aerobic MFCs before the gas swapping with very poor I max and P max [24].…”

Section: Daymentioning

confidence: 99%

“…A more detail work was done by Li et al [3] on the response of Shewanella decolorationis with oxygen within six days of changing the flowing of argon gas and air into the MFCs to create alternate condition of aerobic and anaerobic. They discovered that in contact with oxygen, S. decolorationis reduced more NAD to NADH, which in turns increase in charge production.…”

Section: Introductionmentioning

confidence: 99%

“…Oxygen and nitrate are preferred electron acceptor over the anode reducing power generation in their presence in the anolyte. Tests on the impact of oxygen in air on anaerobic MFCs had been done on both single cell culture, such as from the genus Shewanella [2,3], Escherichia coli (E. coli) [4] and mixed bacterial cultures from anaerobic wastewater treatment streams [5,6]. It was shown that the dissolved oxygen in anolyte reduces the capability of certain bacteria to transfer electrons to external anode.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Long Time Oxygen Exposure on Power Generation of Microbial Fuel Cell With Enriched Mixed Culture

Bakar¹,

Pasco

Gooneratne

et al. 2016

MJAS

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In this study, we are interested in the effect of long time exposure of the microbial fuel cells (MFCs) to air on the electrochemical performance. Here, MFCs enriched using an effluent from a MFC operated for about eight months. After 30 days, the condition of these systems was reversed from aerobic to anaerobic and vice versa, and their effects were observed for 11 days. The results show that for anaerobic MFCs, power generation was reduced when the anodes were exposed to dissolved oxygen of 7.5 ppm. The long exposure of anodic biofilm to air led to poor electrochemical performance. The power generation recovered fully when air supply stopped entering the anode compartment with a reduction of internal resistance up to 53%. The study was able to show that mixed facultative microorganism able to strive through the aerobic condition for about a month at 7.5 ppm oxygen or less. The anaerobic condition was able to turn these microbes into exoelectrogen, producing considerable power in relative to their aerobic state.Keywords: microbial fuel cell, aerobic, oxygen exposure, wastewater AbstrakDalam kajian ini, kami berminat untuk mengesan prestasi elektrokimia sel bahan api mikrob (MFC) terhadap pendedahan jangka masa panjang kepada udara. Di sini, MFC diperkaya menggunakan efluen daripada MFC yang telah beroperasi selama kira -kira lapan bulan. Selepas 30 hari, keadaan sistem ini telah diterbalikkan dari aerobik untuk anaerobik dan sebaliknya, dan kesannya diperhatikan selama 11 hari. Keputusan menunjukkan bahawa untuk MFC anaerobik, penjanaan kuasa telah berkurangan apabila anod terkena oksigen terlarut 7.5 ppm. Pendedahan jangka masa panjang biofilem anod kepada udara membawa kepada prestasi elektrokimia yang rendah. Penjanaan kuasa pulih sepenuhnya apabila bekalan udara berhenti memasuki ruangan anod dengan pengurangan rintangan sehingga 53 %. Kajian ini dapat menunjukkan bahawa mikroorganisma fakultatif campuran dapat hidup melalui keadaan aerobik selama sebulan pada 7.5 ppm oksigen atau kurang. Keadaan anaerobik mampu mengubah mikrob ini kepada eksoelektrogen, seterusnya menghasilkan kuasa yang tinggi berbanding dengan apabila berada di dalam keadaan aerobik.Kata kunci: sel bahan api mikrob, aerobik, pendedahan oksigen, air sisa

show abstract

Effects of oxygen on Shewanella decolorationis NTOU1 electron transfer to carbon-felt electrodes

Cited by 31 publications

References 42 publications

Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquinones

Rate enhancement of bacterial extracellular electron transport involves bound flavin semiquinones

Spectroelectrochemical Investigation on Biological Electron Transfer Associated with Anode Performance in Microbial Fuel Cells

Effect of Long Time Oxygen Exposure on Power Generation of Microbial Fuel Cell With Enriched Mixed Culture

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