Effects of Ozone Exposure on Experimental Asthma in Guinea Pigs Sensitized with Ovalbumin through the Airway

Sumitomo, Midori; Nishikawa, Masanori; Fukuda, T; Kaneko, Tamotsu; Ikeda, Hirotada; Suzuki, Shunsuke; Ōkubo, Takao

doi:10.1159/000235293

Cited by 16 publications

(12 citation statements)

References 12 publications

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“…Moreover, as reported above, when they are exposed after sensitization, the response to airway challenge occurs at lower antigen doses [49,51]. Facilitation of sensitization to ovalbumin has also been reported after exposure to 40 ppm NO 2 [150].…”

Section: Defence Against Infectionsmentioning

confidence: 79%

“…A reversible increase in airway responsiveness to several contractile agents is observed in animals after acute exposure to a concentration of O 3 ranging 0.5-3 ppm [35,38,[43][44][45][46][47][48][49][50][51][52]. Increased airway responsiveness to histamine has also been reported following exposure to 4 ppm or higher concentrations of NO 2 in guinea-pigs [53][54].…”

Section: Effects Of Lung Function and Airway Responsivenessmentioning

confidence: 99%

“…The response to platinum, ovalbumin and Ascaris is enhanced by O 3 in platinum-sensitized monkeys, ovalbumin-sensitized guinea-pigs and Ascaris-sensitized dogs, respectively, [48][49][50][51]. Like the hyperresponsiveness to intravenous histamine, the increase in airway responsiveness to Ascaris antigen induced by ozone in Ascaris suum allergic dogs is not inhibited by the thromboxanesynthase inhibitor OKY-046, suggesting that thromboxane may not be involved [48].…”

Section: Effects Of Lung Function and Airway Responsivenessmentioning

confidence: 99%

“…O 3 exposure has also been shown to reduce the antigen sensitization threshold in guinea-pigs [51]; animals exposed to 5 ppm O 3 before sensitization need less ovalbumin to reach sensitization and show greater antibody production. Moreover, as reported above, when they are exposed after sensitization, the response to airway challenge occurs at lower antigen doses [49,51].…”

Section: Defence Against Infectionsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of oxidant air pollutants on the respiratory system: insights from experimental animal research

Chitano¹,

Hosselet²,

Mapp³

et al. 1995

Eur Respir J

100

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In the present paper, we have reviewed experimental animal studies on the effects of the two most important oxidant airborne pollutants, nitrogen dioxide and ozone, on the respiratory system.The toxic effects depend on concentration and length of exposure, and are generally similar for both oxidants, with ozone operative at lower concentrations. High doses of both oxidants cause death due to lung oedema Exposure to sublethal levels causes functional alterations such as airflow limitation and airway hyperresponsiveness to bronchoconstrictor stimuli. These effects, which are generally reversible, are associated with epithelial injury, oedema and airway and parenchymal infiltration by inflammatory cells. Loss of cilia of airway epithelium and necrosis of type I alveolar epithelial cells are the most prominent consequences at the epithelial level. Inmation is characterized by early neutrophilic infiltration, followed by an increased number of mononuclear cells, predominantly alveolar macrophages.After long-term exposure, whilst nitrogen dioxide causes predominantly emphysema, ozone produces mainly pulmonary fibrosis. Biochemical effects include lipid peroxidation, increased antioxidant metabolism, and alteration of enzyme activity. Nitrogen dioxide and ozone may also alter the immunological response and reduce the defence against infections, increasing the susceptibility of exposed animals to infections.

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Section: Defence Against Infectionsmentioning

confidence: 79%

Section: Effects Of Lung Function and Airway Responsivenessmentioning

confidence: 99%

Section: Effects Of Lung Function and Airway Responsivenessmentioning

confidence: 99%

Section: Defence Against Infectionsmentioning

confidence: 99%

See 2 more Smart Citations

Effect of oxidant air pollutants on the respiratory system: insights from experimental animal research

Chitano¹,

Hosselet²,

Mapp³

et al. 1995

Eur Respir J

100

View full text Add to dashboard Cite

show abstract

“…In addition, other experimental da ta indicate that ozone may enhance the allergic response in the airways. Thus, in the guinea pig, ozone enhances the sensitisation response to ovalbumin (OA) when inhaled via the airways [7] and, following sensitisation, re-exposure to OA leads to an increase in dyspneic episodes and in bronchoconstrictor responses [8,9], Similarly, in mildly allergic asthmatics, exposure to ozone leads to an increase in the immediate bronchoconstrictor response to allergen chal lenge [10].…”

Section: Introductionmentioning

confidence: 99%

Interaction of Ozone and Allergen Challenges on Bronchial Responsiveness and Inflammation in Sensitised Guinea Pigs

Sun

Kojima

Chung

1997

Int Arch Allergy Immunol

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Background: Environmental pollutants such as ozone may interact with airway responses to allergen in sensitised individuals. Methods: We examined the effects of a single exposure to ozone (1 ppm for 1 h) on bronchial responsiveness to acetylcholine (ACh) aerosol 3 and 24 h after single ovalbumin (OA) challenge in OA-sensitised anaesthetised guinea pigs. Broncho-alveolar lavage (BAL) was performed and protein content and differential cell counts were determined. Results: Ozone increased bronchial responsiveness at 3 h but not at 24 h, while OA alone had no effect. At 3 h after ozone, OA induced further, but non-significant increases in bronchial responsiveness to ACh, but at 24 h after ozone, there was enhanced responsiveness. Neutrophil counts in BAL fluid increased at 3 and 24 h after exposure to ozone alone, but there were no further increases with ozone followed by OA exposure. Protein concentration was significantly increased in the ozone and OA groups at 3 and 24 h (163.4 ± 25.6 and 128.7 ± 7.4 mg/ml, respectively) compared to 54.0 ± 18.1 mg/ml in the control group (p < 0.02 and p < 0.01, respectively). Conclusion: Our data demonstrate an interaction of OA with ozone exposure on bronchial responsiveness; one underlying mechanism could be through damage at the endothelial-epithelial barrier.

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Nasal allergy-like symptoms aggravated by ozone exposure in a concentration-dependent manner in guinea pigs

Iijima

Kobayashi

2004

Toxicology

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Our previous study revealed that exposure to 0.4 ppm ozone (O(3)) enhanced nasal allergy-like reactions in guinea pigs. In the present study, we investigated the concentration-dependency of the effects of exposure to O(3) on the aggravation of nasal allergy-like reactions induced by repeated nasal administration of antigen. Guinea pigs were exposed to filtered air or 0.1-0.6 ppm O(3) for 5 weeks. After each weekly administration of ovalbumin (OVA), sneezes and nasal secretions were measured. The number of eosinophils infiltrating the nasal septum and the titers of OVA-specific antibody were measured 24h after the last administration. Ozone increased sneezing and nasal secretion induced by OVA, nasal responsiveness to physical stimuli, and the number of infiltrating eosinophils in a concentration-dependent manner. The titer of anti-OVA-IgG was increased in animals exposed to 0.6 ppm O(3). Thus, exposure to O(3) aggravated nasal allergy-like symptoms concentration dependently. The aggravation was caused by induction of nasal hyperresponsiveness, the infiltration of eosinophils, and the increase in the production of anti-OVA-IgG. The estimated maximum likelihood estimation concentrations (MLECs) and bench mark concentrations (BMCs) of O(3) for these indices were in the range of 0.09-0.18 and 0.02-0.06 ppm, respectively.

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Effects of Ozone Exposure on Experimental Asthma in Guinea Pigs Sensitized with Ovalbumin through the Airway

Cited by 16 publications

References 12 publications

Effect of oxidant air pollutants on the respiratory system: insights from experimental animal research

Effect of oxidant air pollutants on the respiratory system: insights from experimental animal research

Interaction of Ozone and Allergen Challenges on Bronchial Responsiveness and Inflammation in Sensitised Guinea Pigs

Nasal allergy-like symptoms aggravated by ozone exposure in a concentration-dependent manner in guinea pigs

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