Effects of Ozone Oxidative Preconditioning on TNF‐α Release and Antioxidant‐Prooxidant Intracellular Balance in Mice During Endotoxic Shock

Abstract

Ozone oxidative preconditioning is a prophylactic approach, which favors the antioxidant-prooxidant balance for preservation of cell redox state by the increase of antioxidant endogenous systems in both in vivo and in vitro experimental models. Our aim is to analyze the effect of ozone oxidative preconditioning on serum TNF-α levels and as a modulator of oxidative stress on hepatic tissue in endotoxic shock model (mice treated with lipopolysaccharide (LPS)). Ozone/oxygen gaseous mixture which was administered intraperitoneally (0.2, 0.4, and 1.2 mg/kg) once daily for five days before LPS (0.1 mg/kg, intraperitoneal). TNF-α was measured by cytotoxicity on L-929 cells. Biochemical parameters such as thiobarbituric acid reactive substances (TBARS), enzymatic activity of catalase, glutathione peroxidase, and glutathione-S transferase were measured in hepatic tissue. One hour after LPS injection there was a significant increase in TNF-α levels in mouse serum. Ozone/oxygen gaseous mixture reduced serum TNF-α levels in a dose-dependent manner. Statistically significant decreases in TNF-α levels after LPS injection were observed in mice pretreated with ozone intraperitoneal applications at 0.2 (78%), 0.4 (98%), and 1.2 (99%). Also a significant increase in TBARS content was observed in the hepatic tissue of LPS-treated mice, whereas enzymatic activity of glutathion-S transferase and glutathione peroxidase was decreased. However in ozone-treated animals a significant decrease in TBARS content was appreciated as well as an increase in the activity of antioxidant enzymes. These results indicate that ozone oxidative preconditioning exerts inhibitory effects on TNF-α production and on the other hand it exerts influence on the antioxidant-prooxidant balance for preservation of cell redox state by the increase of endogenous antioxidant systems.

Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

“…Por otra parte, en simulaciones de shock endotóxico, el ozono protege a la rata disminuyendo TNF-α sanguíneo, esto se explicaría porque los antioxidantes generados por el ozono, administrado antes de la lesión, disminuyen el NF-kB y las citoquinas circulantes 39,40 .…”

La ozonoterapia en pacientes con enfisema pulmonar

“…De esta manera podemos ver el efecto inmunoregulador de la ozonoterapia, el cual contribuye a la disminución del proceso inflamatorio en nuestra patología 31 y a la mejoría de la función pulmonar. además, la ozonoterapia posee un poderoso efecto estimulador de las defensas antioxidantes 13,[16][17][18][19][20][21] , que impide la progresión del proceso destructivo del tejido pulmonar.…”

“…así también, en la activación de la actividad antioxidante endó-gena, como inmunoregulador, y su efecto en la elevación en sangre del 2,3 disfosfoglicerato, por esta vía. Por el contrario, la ozonoterapia no es recomendable por vía inhalatoria ya que en altas concentraciones es tóxica, sobre todo para el sistema respiratorio [13][14][15][16][17][18][19] .…”

“…a nivel preclínico se ha observado una modulación a nivel de tejido pulmonar lesionado de la enzima mieloperoxidasa. Todos estos aspectos hacen pensar que la aplicación del ozono en pacientes con EPOc, podría tener un efecto beneficioso [13][14][15][16][17][18][19][20][21] .…”

Therapeutic Efficacy of Mammary Irrigation Regimen in Dairy Cattle Diagnosed with Acute Coliform Mastitis

“…It was reported that treatment with ozone released fewer endotoxins in the milk than treatment with antibiotics; further, no iatrogenic endotoxic shock occurred as an adverse effect, unlike that in the case of antibiotic treatment [10]. In addition, it has been reported that ozone may result in the downregulation of the cytotoxic activity of immunocytes [1,21,22]. Therefore, we speculated that using ozone water as an irrigation fluid during MIR as the first line of treatment for coliform mastitis was possible to reduce mortality.…”