Effects of ozonized glycerin on inflammation of mammary glands induced by intramammary lipopolysaccharide infusion in goats

Abstract

Although ozone shows antimicrobial activity against mastitis-causing pathogens in ruminants, its anti-inflammatory effect on mammary glands remains unclear. The aim of this study was to examine the anti-inflammatory effects of ozonized glycerin (OG) on experimentally induced inflammation in the mammary glands of six Shiba and two Tokara lactating goats. We infused 1 μg lipopolysaccharide (LPS) into all udders on day À1. On day 0, post LPS infusion, OG (ozone group), and glycerin (control group) were infused into the right and left sides of the udders, respectively.Milk samples were collected once daily from days À1 to 7. The somatic cell count and lactoperoxidase (LPO) activity, along with the interleukin-1β (IL-1β), tumor necrosis factor-α, IL-8, IL-10, lactoferrin, and sodium ion concentrations in milk were measured. IL-8, IL-10, and lactoferrin levels after LPS infusion in the ozone group were significantly lower than those in the control group, and the LPO activity tended to be lower than that observed in the control group. This study showed that OG has anti-inflammatory potential against LPS-induced inflammation in the mammary glands.

In Vitro Activity of Ozone/Oxygen Gaseous Mixture against a Caprine Herpesvirus Type 1 Strain Isolated from a Goat with Vaginitis

“…O 3 therapy seemingly matched or outperformed antibiotics treatments, reducing the risks of antimicrobial resistance [ 34 , 37 ] and withdrawal times for meat and milk because it does not leave residue in biological tissue [ 37 ]. In goat medicine, few studies have been conducted on the application of O 3 therapy, and they are mainly focused on reproductive [ 34 ] and milk production [ 38 ] performances.…”