Effects of p-Cresol on Senescence, Survival, Inflammation, and Odontoblast Differentiation in Canine Dental Pulp Stem Cells

Zayed, Mohammed; Iohara, Koichiro

doi:10.3390/ijms21186931

Cited by 4 publications

(2 citation statements)

References 63 publications

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“…Dental tissue-derived cells Long-term stress on teeth may induce the human dental pulp cells senescence and up-regulation of SASP factors in human dental pulp cells ( 32 ). In aging patients, dental pulp stem cells exhibit elevated expression of SASP factors ( 33 ). Dental follicle cells are positive for SA-β-gal staining in later stages of cell culture ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

Senescence-associated secretory phenotype and its impact on oral immune homeostasis

et al. 2022

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The senescence-associated secretory phenotype (SASP), which accumulates over the course of normal aging and in age-related diseases, is a crucial driver of chronic inflammation and aging phenotypes. It is also responsible for the pathogenesis of multiple oral diseases. However, the pathogenic mechanism underlying SASP has not yet been fully elucidated. Here, relevant articles on SASP published over the last five years (2017–2022) were retrieved and used for bibliometric analysis, for the first time, to examine SASP composition. More than half of the relevant articles focus on various cytokines (27.5%), growth factors (20.9%), and proteases (20.9%). In addition, lipid metabolites (13.1%) and extracellular vesicles (6.5%) have received increasing attention over the past five years, and have been recognized as novel SASP categories. Based on this, we summarize the evidences demonstrating that SASP plays a pleiotropic role in oral immunity and propose a four-step hypothetical framework for the progression of SASP-related oral pathology—1) oral SASP development, 2) SASP-related oral pathological alterations, 3) pathological changes leading to oral immune homeostasis disruption, and 4) SASP-mediated immune dysregulation escalating oral disease. By targeting specific SASP factors, potential therapies can be developed to treat oral and age-related diseases.

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Section: Discussionmentioning

confidence: 99%

Senescence-associated secretory phenotype and its impact on oral immune homeostasis

et al. 2022

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“…The uremic toxin p-Cresol (PC) could induce cellular senescence of dental pulp stem cells, reflecting the ageing changes in dental pulp [144]. This exposure promoted the distraction of the cell cycle (with increased Bax protein and decreased Bcl-2 levels) and the inflammatory processes (with elevated IL-6 expression).…”

Section: The Ageing Influence On the Metabolic Functioning Of The Pul...mentioning

confidence: 99%

The Role of Cellular Metabolism in Maintaining the Function of the Dentine-Pulp Complex: A Narrative Review

et al. 2023

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The cellular metabolic processes ensure the physiological integrity of the dentine-pulp complex. Odontoblasts and odontoblast-like cells are responsible for the defence mechanisms in the form of tertiary dentine formation. In turn, the main defence reaction of the pulp is the development of inflammation, during which the metabolic and signalling pathways of the cells are significantly altered. The selected dental procedures, such as orthodontic treatment, resin infiltration, resin restorations or dental bleaching, can impact the cellular metabolism in the dental pulp. Among systemic metabolic diseases, diabetes mellitus causes the most consequences for the cellular metabolism of the dentine-pulp complex. Similarly, ageing processes present a proven effect on the metabolic functioning of the odontoblasts and the pulp cells. In the literature, several potential metabolic mediators demonstrating anti-inflammatory properties on inflamed dental pulp are mentioned. Moreover, the pulp stem cells exhibit the regenerative potential essential for maintaining the function of the dentine-pulp complex.

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Age Related Senescence, Apoptosis, and Inflammation Profiles in Periodontal Ligament Cells from Canine Teeth

Zayed

Iohara

2023

CMM

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Objective: The periapical tissues including periodontal ligament cells (PDLCs) are playing an important role in repairing the surrounding tissue of the teeth. A decrease in the regenerative potentiality of resident stem cells (PDLCs) has been suggested to be attributed to the decline of pulp regeneration. Therefore, it is necessary to examine the functional changes in periodontal tissue and cells that occur during the aging process. Methods: The changes in the cementum extract (CE) and PDLCs isolated from young and aged dog teeth were evaluated. PDLCs growth rate, senescence markers, p16 and p21, and proinflammatory cytokines, IL-6, IL-1β, and TNF-α were analyzed by RT-PCR. Bax, an apoptosis marker, Bcl-2, a marker for cell survival, and IL-6 were examined by Western blot analyses to detect their variance expression in the CE. Results: Our results demonstrated that aged PDLCs exhibit low growth rate and an increased expression of p16, however, no change has been demonstrated in the expression of p21. The chronic inflammatory molecules, IL-6 and TNF-α were significantly upregulated compared to young PDLCs. Western blot analyses showed decreased expression of Bcl-2 in the CE of the aged tooth (p < 0.001). Conclusion: Taken together, aging influences the functional changes of PDLCs and CE and increases senescence, chronic inflammation, and apoptosis markers. As a result, donor age is a key factor influencing the utilization of PDLCs for tooth regeneration.

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Effects of p-Cresol on Senescence, Survival, Inflammation, and Odontoblast Differentiation in Canine Dental Pulp Stem Cells

Cited by 4 publications

References 63 publications

Senescence-associated secretory phenotype and its impact on oral immune homeostasis

Senescence-associated secretory phenotype and its impact on oral immune homeostasis

The Role of Cellular Metabolism in Maintaining the Function of the Dentine-Pulp Complex: A Narrative Review

Age Related Senescence, Apoptosis, and Inflammation Profiles in Periodontal Ligament Cells from Canine Teeth

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