Effects of Parathyroid Hormone Related Peptide (1 - 34) and 1,25-Dihydroxyvitamin D on Bone Alkaline Phosphatase Activity in Fetal Rat Calvaria Cultures

Municio, M. J.; Traba, M. L.; Piedra, Concepción de la

doi:10.1055/s-2007-979963

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2004

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“…Catvaria (one/well/lml medium) were incubated in multiwell plates for 24, 28 or 72 h at 37 ~ in 5% COffair atmosphere. Medium was changed every 24 h (10).…”

Section: Methodsmentioning

confidence: 99%

Unión específica de 1,25(OH)2D3 a hueso en la deficiencia en magnesio

Risco

Traba

2004

J. Physiol. Biochem.

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It has been reported that some hypoparathyroid patients with magnesium deficiency showed altered responses to vitamin D treatment. In the same way, in vitro bone studies have demonstrated the existence of a decrease in the 1,25-dihydroxyvitamin D3-induced resorption in bone as a result of magnesium deficiency. These findings suggest some kind of alteration in the 1,25(OH)2D3 in bone in magnesium deficiency. In the present work, using a binding assay based on the 1,25(OH)2D3 and 3H-1,25(OH)2D3 competition for the hormone binding sites in rat calvaria homogenates, a significant decrease in the number of 1,25(OH)2D3 specific binding sites has been found in calvaria incubated in magnesium-deficient medium compared to magnesium-replete ones. Alterations in the hormone-receptor affinity were not found. These results suggest that an alteration in the 1,25(OH)2D3 action on magnesium-deficient bone could be due, at least in part, to a decrease in the number of available vitamin D receptors in bone cells.

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“…Catvaria (one/well/lml medium) were incubated in multiwell plates for 24, 28 or 72 h at 37 ~ in 5% COffair atmosphere. Medium was changed every 24 h (10).…”

Section: Methodsmentioning

confidence: 99%