Effects of Parboiled Rice Diet on Oxidative Stress Parameters in Kidney of Rats with Streptozotocin-Induced Diabetes

Abstract

The effect of parboiled rice (PR) and white rice (WR) diets on oxidative stress (OS) parameters was investigated in the kidneys of rats with streptozotocin-induced diabetes (40 mg kg(-1), iv). The experimental groups (n=8) were control fed with PR (CPR), control fed with WR, diabetic fed with PR, and diabetic fed with WR. After 30 days of treatment, all animals were anesthetized and exsanguinated before removal of kidneys, which were used to determine thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides, carbonyl protein, superoxide dismutase, catalase, glutathione peroxidase (GPx), glutathione reductase, glutathione-S-transferase activities, and levels of glutathione (GSH). Total phenolic compounds were determined in WR and PR grains. Our data indicated that diabetes induced increase in TBARS and lipid hydroperoxides levels. Although PR has not prevented the rise in the levels of these measurements, its consumption by our animals resulted in higher GPx activity and GSH content than that of the CPR. Moreover, PR also presented concentration of total phenolic compounds 127% higher than WR grains. Thus, its consumption in this diabetic condition is suggested because this seems to confer greater protection against OS in the renal tissue of diabetic animals.

The phytochemical, EGCG, extends lifespan by reducing liver and kidney function damage and improving age‐associated inflammation and oxidative stress in healthy rats

“…Liver and kidney tissues were removed, cleaned, and washed in ice‐cold normal saline. The tissues were homogenized in 1.15% (w/v) potassium chloride containing 1 mmol/L phenylmethylsulfonyl fluoride, centrifuged at 3000 rpm for 15 min, the supernatant was filtered through a 0.2‐mm membrane filter, and the filtrate was analyzed (Finamor et al ., ). SOD, GSH‐Px, MDA, TNF‐α, IL‐6, and ROS were measured with commercial kits using previously published methods.…”

Periapical Lesions Decrease Insulin Signaling in Rat Skeletal Muscle

“…The blood samples were centrifuged immediately after collection at 1800g for 15 minutes at 4 C to obtain serum (25). Serum total cholesterol, triglyceride, and glucose levels were measured enzymatically by using a commercial kit (Colesterol Liquiform Labtest, Triglic erides Liquiform Labtest, and Glicose Liquiform Labtest, respectively; Labtest Diagn ostica Ind e Com Ltda, Lagoa Santa, MG, Brazil) (23,26).…”

Antihyperglycemic and Antioxidant Activity of Crocin in Streptozotocin-Induced Diabetic Rats

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