Effects of PDMS curing ratio and 3D micro-pyramid structure on the formation of an in vitro neural network

Choi, Ahmi; Kim, Jae Young; Lee, Jong Eun; Jung, Hyo Il

doi:10.1016/j.cap.2009.06.029

Cited by 7 publications

(3 citation statements)

References 6 publications

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“…This in part may be due to the different mechanical environments that cells are exposed to in the efforts to generate these 3D cultures. Mechanical properties such as elastic modulus, − conductivity, , and wettability have all been demonstrated to influence cellular behaviors and thus material properties likely influence the increased physiological responses that cells exhibit.…”

Section: Introductionmentioning

confidence: 99%

Aligned Poly-l-lactic Acid Nanofibers Induce Self-Assembly of Primary Cortical Neurons into 3D Cell Clusters

Weir

Stevens

Wagner

et al. 2022

ACS Biomater. Sci. Eng.

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Relative to two-dimensional (2D) culture, three-dimensional (3D) culture of primary neurons has yielded increasingly physiological responses from cells. Electrospun nanofiber scaffolds are frequently used as a 3D biomaterial support for primary neurons in neural tissue engineering, while hydrophobic surfaces typically induce aggregation of cells. Poly-l-lactic acid (PLLA) was electrospun as aligned PLLA nanofiber scaffolds to generate a structure with both qualities. Primary cortical neurons from E18 Sprague–Dawley rats cultured on aligned PLLA nanofibers generated 3D clusters of cells that extended highly aligned, fasciculated neurite bundles within 10 days. These clusters were viable for 28 days and responsive to AMPA and GABA. Relative to the 2D culture, the 3D cultures exhibited a more developed profile; mass spectrometry demonstrated an upregulation of proteins involved in cortical lamination, polarization, and axon fasciculation and a downregulation of immature neuronal markers. The use of artificial neural network inference suggests that the increased formation of synapses may drive the increase in development that is observed for the 3D cell clusters. This research suggests that aligned PLLA nanofibers may be highly useful for generating advanced 3D cell cultures for high-throughput systems.

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Section: Introductionmentioning

confidence: 99%

Aligned Poly-l-lactic Acid Nanofibers Induce Self-Assembly of Primary Cortical Neurons into 3D Cell Clusters

Weir

Stevens

Wagner

et al. 2022

ACS Biomater. Sci. Eng.

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“…In recent years, there has been increasing demand for largearea polymer films with micro structures, such as in flexible displays, e-paper, flexible solar cells and so forth [1]. Micropyramid arrays, due to merits of large specific surface areas and good optical characteristics including multiple refraction and reflection [2], have wide application prospects for biology [3][4][5], chemistry [6] and especially for micro-optical elements [7][8][9]. Taking some micro-optical elements with micropyramids as examples, pyramidal reflectors on solar cells [10] led to a more than 2.5 times longer path length in the active layer for incident light, compared with a planar rear reflector.…”

Section: Introductionmentioning

confidence: 99%

Experimental investigation on the large-area fabrication of micro-pyramid arrays by roll-to-roll hot embossing on PVC film

Deng

Peng

et al. 2014

J. Micromech. Microeng.

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Large-area polymeric components with micro-pyramids have been widely applied in the fields of optics, optoelectronics, biology and chemistry, etc. Roll-to-roll (R2R) hot embossing is regarded as a promising approach to fulfil high throughput fabrication of patterned polymeric films. In this study, an R2R hot embossing system has been developed in-house and effective and continuous production of the polymeric component with micro-pyramids is demonstrated by R2R hot embossing. The influence of processing parameters has been firstly investigated by using the one-variable-at-a-time method. Afterwards, a series of experiments based on the central composite design approach have been conducted for the analysis of variance and the establishment of empirical models of the R2R hot embossing process. As a result, a 90 mm × 90 mm PVC sample with a feature height of 65 µm was successfully fabricated and the height consistency reached 94.5%. Additionally, a process window with a mold temperature of 150–160 °C, an applied force of 18–25 kgf and a feeding speed of 0.3–0.5 m min−1, was established to achieve 100% passable micro-pyramid arrays. The processing rules and the concrete ranges of parameter values can guide the process production of large-area micro-pyramids.

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“…The technique used the effect of hydrophobic and hydrophilic on the surface with different surface energy levels to the fluids [10] [11]. The ink was made by polydimethylsiloxane (PDMS) (curing ratio 10:1) [12], using xylene as solvent (mixing ratio, 2:1 by volume) which was printed onto the substrate forming the negative patterns. Then, the printed patterns were cured on a hotplate at 120°C for 60mins.…”

mentioning

confidence: 99%

Gravure printed hydrophobic templates onto PET films for guiding the assembly of nanowires: Towards the ultralow-cost transparent conductive electrodes

Wong

Yang

2011

2011 IEEE 61st Electronic Components and Technology Conference (ECTC)

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We present our work of fabricating silver nanowires (Ag NWs) network with regularity and homogeneity as the transparent conductive electrodes for optoelectronic applications. Though patterning hydrophobic materials (e.g. PDMS) onto the substrate (polyethylene terephthalate (PET)) surface, we are able to adjust the surface energy level between the patterned and un-patterned area, which is crucial to the wetting/dewetting of the solution of the Ag NWs. This allows us to control the distribution of the percolated Ag NWs network evenly in the unprinted areas. In this way, we can fabricate transparent electrode very conveniently through a general printing method coupled with a deposition-drying process. The Ag NW network displays 2-D electrical conductivity and excellent optical transparency, which provides promising alternatives to ITO as transparent conductive electrodes.

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Effects of PDMS curing ratio and 3D micro-pyramid structure on the formation of an in vitro neural network

Cited by 7 publications

References 6 publications

Aligned Poly-l-lactic Acid Nanofibers Induce Self-Assembly of Primary Cortical Neurons into 3D Cell Clusters

Aligned Poly-l-lactic Acid Nanofibers Induce Self-Assembly of Primary Cortical Neurons into 3D Cell Clusters

Experimental investigation on the large-area fabrication of micro-pyramid arrays by roll-to-roll hot embossing on PVC film

Gravure printed hydrophobic templates onto PET films for guiding the assembly of nanowires: Towards the ultralow-cost transparent conductive electrodes

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