Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in <i>Catharanthus</i> <i>roseus</i>

Liu, Ying; Meng, Qinghuan; Xi-Hua, Duan; Zhang, Zhonghua; Li, Dewen

doi:10.1080/17429145.2017.1293852

Cited by 41 publications

(23 citation statements)

References 28 publications

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“…In contrast to our results, Liu et al [73] demonstrated an increased accumulation of alkaloids and the expression of genes regulating secondary metabolite accumulation in drought-stressed C. roseus. However, reports discussing the combined effects of drought and heat stress on the accumulation of secondary metabolites are rare.…”

Section: Effects Of Drought and Heat Stress On The Growth And Physiolcontrasting

confidence: 99%

Changes in Ecophysiology, Osmolytes, and Secondary Metabolites of the Medicinal Plants of Mentha piperita and Catharanthus roseus Subjected to Drought and Heat Stress

et al. 2019

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Global warming contributes to higher temperatures and reduces rainfall for most areas worldwide. The concurrent incidence of extreme temperature and water shortage lead to temperature stress damage in plants. Seeking to imitate a more natural field situation and to figure out responses of specific stresses with regard to their combination, we investigated physiological, biochemical, and metabolomic variations following drought and heat stress imposition (alone and combined) and recovery, using Mentha piperita and Catharanthus roseus plants. Plants were exposed to drought and/or heat stress (35 °C) for seven and fourteen days. Plant height and weight (both fresh and dry weight) were significantly decreased by stress, and the effects more pronounced with a combined heat and drought treatment. Drought and/or heat stress triggered the accumulation of osmolytes (proline, sugars, glycine betaine, and sugar alcohols including inositol and mannitol), with maximum accumulation in response to the combined stress. Total phenol, flavonoid, and saponin contents decreased in response to drought and/or heat stress at seven and fourteen days; however, levels of other secondary metabolites, including tannins, terpenoids, and alkaloids, increased under stress in both plants, with maximal accumulation under the combined heat/drought stress. Extracts from leaves of both species significantly inhibited the growth of pathogenic fungi and bacteria, as well as two human cancer cell lines. Drought and heat stress significantly reduced the antimicrobial and anticancer activities of plants. The increased accumulation of secondary metabolites observed in response to drought and/or heat stress suggests that imposition of abiotic stress may be a strategy for increasing the content of the therapeutic secondary metabolites associated with these plants.

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Section: Effects Of Drought and Heat Stress On The Growth And Physiolcontrasting

confidence: 99%

Changes in Ecophysiology, Osmolytes, and Secondary Metabolites of the Medicinal Plants of Mentha piperita and Catharanthus roseus Subjected to Drought and Heat Stress

et al. 2019

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“…Drought has been shown to cause dynamic variations in the levels of vindoline and catharanthine, wherein vindoline displayed a decline-rise trend while catharanthine showed a gradual decline in its levels in C . roseus tissues subjected to PEG-induced drought stress 81 . Geraniol content was found to decrease upon drought treatment.…”

Section: Resultsmentioning

confidence: 99%

Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus

Nishanth

Sheshadri

Rathore

et al. 2018

Sci Rep

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Catharanthus roseus is a commercial source for anti-cancer terpenoid indole alkaloids (TIAs: vincristine and vinblastine). Inherent levels of these TIAs are very low, hence research studies need to focus on enhancing their levels in planta. Since primary metabolism provides precursors for specialized-metabolism, elevating the former can achieve higher amounts of the latter. Cell Wall Invertase (CWIN), a key enzyme in sucrose-metabolism catalyses the breakdown of sucrose into glucose and fructose, which serve as carbon-skeleton for specialized-metabolites. Understanding CWIN regulation could unravel metabolic-engineering approaches towards enhancing the levels of TIAs in planta. Our study is the first to characterize CWIN at gene-expression level in the medicinal plant, C. roseus. The CWINs and their inter-relationship with sucrose and TIA metabolism was studied at gene and metabolite levels. It was found that sucrose-supplementation to C. roseus leaves significantly elevated the monomeric TIAs (vindoline, catharanthine) and their corresponding genes. This was further confirmed in cross-species, wherein Nicotiana benthamiana leaves transiently-overexpressing CrCWIN2 showed significant upregulation of specialized-metabolism genes: NbPAL2, Nb4CL, NbCHS, NbF3H, NbANS, NbHCT and NbG10H. The specialized metabolites- cinnamic acid, coumarin, and fisetin were significantly upregulated. Thus, the present study provides a valuable insight into metabolic-engineering approaches towards augmenting the levels of therapeutic TIAs.

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“…Alkaloids accumulation recorded following stressful Co 2+ treatments is a defense response against oxidative stress (13). It was recorded in C. roseous following exposure to different types of stress including heavy metals (14)(15)(16)(17), drought (53) and fungal attack (54).…”

Section: Discussionmentioning

confidence: 99%

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Fouad

Hafez

2018

Cell Mol Biol (Noisy-le-grand)

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The capabilities of cobalt ions (Co 2+) and cobalt nanoparticles (CoNPs) in enhancing alkaloids accumulation in Catharanthus roseus suspension cultures were evaluated in relation to the expression of CrMPK3 gene. Four concentrations (5, 10, 15 and 20 mg/L) of each cobalt form were utilized in addition to control. Both forms induced oxidative stress that was more pronounced for Co 2+ treatments. A positive correlation was observed between cobalt concentration and expression of CrMPK3 gene. However, a characteristic temporal expression profile was recorded for each cobalt form. Also, positive correlations were detected between both cobalt concentration and expression of CrMPK3 gene on one hand and the activities of antioxidant enzymes (SOD and APX) and alkaloids content on the other hand. Such correlations suggest CrMPK3 gene as a common player in cobalt-induced stress signaling; regardless of cobalt form. Results revealed the higher capability of Co 2+ , compared with CoNPs, in enhancing alkaloids accumulation. However, results supported CoNPs as a novel tool in manipulating cobaltinduced alkaloids production in C. roseus.

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Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus

Cited by 41 publications

References 28 publications

Changes in Ecophysiology, Osmolytes, and Secondary Metabolites of the Medicinal Plants of Mentha piperita and Catharanthus roseus Subjected to Drought and Heat Stress

Changes in Ecophysiology, Osmolytes, and Secondary Metabolites of the Medicinal Plants of Mentha piperita and Catharanthus roseus Subjected to Drought and Heat Stress

Expression analysis of Cell wall invertase under abiotic stress conditions influencing specialized metabolism in Catharanthus roseus

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

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