Effects of pegagan (Centella asiatica L.) ethanolic extract SNEDDS (self-nanoemulsifying drug delivery systems) on the development of zebrafish (Danio rerio) Embryos

Hayati, Farida; Chabib, Lutfi; It, Silmi Fauzi; Awaluddin, Rizki; Sumayya,; Faizah, Wan Syarifah; Nasir, Moh Hamzah Mohd; Nipun, TS

doi:10.4103/jpbs.jpbs_297_19

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…Zebrafish embryos are ideal models for testing drug toxicity in vivo because their optical transparency enables the monitoring of morphological changes ( Figure 3A ). 53 , 54 After incubation with 250 μg/mL of nGO, TPGS, and RGD-TPGS-nGO, zebrafish embryos were examined at 96 hours post-fertilization (hpf). The treated zebrafish embryos showed no significant differences in survival rate ( Figure 3B ), hatching rate ( Figure 3C ), heart rate ( Figure 3D ), and body length ( Figure 3E ) when compared with the control embryos.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional Graphene Oxide Nanodelivery Platform for Breast Cancer Treatment

Mo¹,

Liu²,

Liu³

et al. 2022

IJN

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Background: Breast cancer (BC) has the highest global prevalence among all malignancies in women and the second highest prevalence in the overall population. Paclitaxel (PTX), a tricyclic diterpenoid, is effective against BC. However, its poor solubility in water and the allergenicity of its dissolution medium limited its clinical application. Methods: In this work, we established a multifunctional graphene oxide (GO) tumor-targeting drug delivery system using nanosized graphene oxide (nGO) modified with D-tocopherol polyethylene glycol succinate (TPGS) and arginine-glycine-aspartic acid (RGD) for PTX loading. Results:The obtained RGD-TPGS-nGO-PTX was 310.20±19.86 nm in size; the polydispersity index (PDI) and zeta potential were 0.21±0.020 and −23.42 mV, respectively. The mean drug loading capacity of RGD-TPGS-nGO-PTX was 48.78%. RGD-TPGS-nGO-PTX showed satisfactory biocompatibility and biosafety and had no significant toxic effects on zebrafish embryos. Importantly, it exerted excellent cytotoxicity against MDA-MB-231 cells, reversed multi-drug resistance (MDR) in MCF-7/ADR cells, and showed significant anti-tumor efficacy in tumor-bearing nude mice. Conclusion: These findings strongly suggested that the multifunctional GO tumor-targeting drug delivery system RGD-TPGS-nGO-PTX could be used in clinical settings to improve PTX delivery, reverse MDR and increase the therapeutic efficacy of BC treatment.

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Section: Resultsmentioning

confidence: 99%

Multifunctional Graphene Oxide Nanodelivery Platform for Breast Cancer Treatment

Mo¹,

Liu²,

Liu³

et al. 2022

IJN

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“…Although brittle star merely contained a minute amount of asterosaponin P1, experiments on zebrafish embryos showed that it was teratogenic and embryotoxic, suggesting that it may have chemical defenses against nearby swimming predators. The toxicity of crude plant extracts containing saponins has been evaluated using the zebrafish embryos model [ 31 , 32 , 33 ], our study firstly provided the evidence that steroidal saponin isolated from marine invertebrates has a high impact on the growth and development of zebrafish embryos and had teratogenic effects on the morphology of zebrafish. Our findings highlight the chemoecological functions of steroidal saponins of echinoderms origin, providing insight into the chemical defenses of marine benthic echinoderms.…”

Section: Discussionmentioning

confidence: 99%

Embryotoxicity and Teratogenicity of Steroidal Saponin Isolated from Ophiopholis mirabilis

Xu¹,

Yang

Zhang

et al. 2023

Toxics

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Benthic invertebrates produce secondary metabolites that serve as defenses against consumers and promote their fitness. To explore the chemical defense in marine benthic echinoderms, the chemical constituents of Ophiopholis mirabilis were investigated. A steroidal monoglycoside, asterosaponin P1, was isolated from O. mirabilis for the first-time using column chromatography. The chemical structure was characterized by spectroscopic and spectrometric methods. The embryotoxicity and teratogenicity of the isolated compound were assessed using the zebrafish embryo assay, a powerful vertebrate animal model system to study mechanisms of toxicity. When applied at high concentrations, asterosaponin P1 causes a significant increase in embryo mortality. A moderate LC50 of asterosaponin P1 appeared to be time- and concentration-dependent in its toxicity to zebrafish embryos. Teratogenicity in zebrafish embryos also included morphological defects, decreased hatchability, and a reduced heart rate. These findings revealed that steroidal saponin extracted from O. mirabilis exhibited acute toxic effects on zebrafish embryos, suggesting a potential chemical defense function in marine habitats.

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“…Corong buchner digunakan untuk menyaring sehingga didapatkan filtrat. Rotary evaporator pada suhu 40 o C untuk menghasilkan ekstrak kental daun pegagan dan ekstrak kental daun sirih merah [40].…”

Section: Pembuatan Snedds-gel Kombinasi Daun Pegagan Dan Daun Sirih M...unclassified

Review: SNEDDS Gel Kombinasi Ekstrak Daun Pegagan dan Daun Sirih Merah Sebagai Agen Penyembuh Luka

Dewi¹,

Yowani²

2023

WSNF

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Luka adalah keadaan terjadi putusnya jaringan tubuh yang menyebabkan fungsi tubuh terganggu dalam melakukan aktivitas. Luka apabila tidak diobati sesuai dengan pengobatan yang benar, maka rentan menyebabkan waktu penyembuhan luka menjadi lama. Ekstrak daun pegagan dan ekstrak daun sirih merah adalah kombinasi bahan aktif yang berpotensi dikembangkan dengan tujuan agar efektivitas penyembuhan luka meningkat, karena mengandung berbagai konstituen yang memiliki indikasi untuk mempercepat proses penyembuhan luka serta antibakteri. Namun, marker utama dari kedua zat aktif ini memiliki kelarutan yang buruk. Self-Nanoemulsifying Drug Delivery System (SNEDDS) adalah suatu sistem penghantaran obat yang stabil sehingga sistem ini mampu membuat kelarutan dan bioavailabilitas zat aktif menjadi meningkat. Kecilnya ukuran partikel zat aktif akan meningkatkan luas permukaan kontak antara zat aktif dan pelarut, maka hal ini akan menyebabkan absorpsi, laju disolusi, serta kelarutan zat aktif meningkat. SNEDDS dibuat dengan mengkombinasikan ekstrak daun pegagan dan daun sirih merah sebagai bahan aktif dengan surfaktan dan ko-surfaktan dengan perbandingan 1: 6: 3 (1 mL capryol 90 (fase minyak), 6-mL tween 20 (surfaktan), dan 3-mL PEG 400 (ko-surfaktan)) lalu diformulasikan ke dalam sediaan gel dengan penggunaan karbopol sebagai gelling agent. Tujuan penelitian ini adalah untuk menambah wawasan serta memperbaharui pengetahuan masyarakat terkait kandungan kimia daun pegagan dan daun sirih merah sebagai agen terapi penyembuhan luka, beserta teknologi pembuatan SNEDDS-gel guna memperoleh hasil terbaik. Produksi agen terapi penyembuh luka berbahan dasar kombinasi kedua ekstrak ini dengan bentuk sediaan SNEDDS-gel berpsotensi dikembangkan menjadi produk herbal fungsional yaitu sebagai agen penyembuhan luka.

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Effects of pegagan (Centella asiatica L.) ethanolic extract SNEDDS (self-nanoemulsifying drug delivery systems) on the development of zebrafish (Danio rerio) Embryos

Cited by 8 publications

References 17 publications

Multifunctional Graphene Oxide Nanodelivery Platform for Breast Cancer Treatment

Multifunctional Graphene Oxide Nanodelivery Platform for Breast Cancer Treatment

Embryotoxicity and Teratogenicity of Steroidal Saponin Isolated from Ophiopholis mirabilis

Review: SNEDDS Gel Kombinasi Ekstrak Daun Pegagan dan Daun Sirih Merah Sebagai Agen Penyembuh Luka

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