Effects of Pentoxifylline, Caffeine, and Taurine on Post-Thaw Motility and Longevity of Equine Frozen Semen

Stephens, T.D.; Brooks, Ryan M.; Carrington, Julia L.; Cheng, Lulu; Carrington, Anne C.; Porr, C. A. Shea; Splan, Rebecca K.

doi:10.1016/j.jevs.2012.10.004

Cited by 35 publications

(47 citation statements)

References 24 publications

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“…Different superscript letters (a-c) indicate significant differences at P < 0.05. On the other hand, the negative effects of the highest concentration of taurine might be caused by high osmotic pressure, as described previously (Stephens et al 2013). from previous studies (Alvarez & Storey 1983;Atessahin et al 2008;Sarı€ ozkan et al 2009).…”

Section: Figuresupporting

confidence: 56%

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Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Zhang

Qian

et al. 2017

Animal Science Journal

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Peroxidation damage induces sublethal injury to boar sperm during the storage process. Taurine has already been demonstrated to protect cells effectively from oxidant-induced injury. This study was aimed to evaluate the effect of different concentrations of taurine (0.5, 1, 5 and 10 mmol/L) in Modena diluent on boar sperm quality during liquid storage at 17°C. Ejaculates from sexually mature Duroc pigs were collected, pooled and preserved in the Modena containing different concentrations of taurine. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidative capacity (T-AOC) activity and malondialdehyde content (MDA) were examined every 24 h. Modena diluent containing taurine suppressed the reduction in sperm qualities during the process of liquid preservation compared with those of the control group. After 5 days of liquid preservation, the addition of taurine at 5 mmol/L had the optimal effect on survival time as well as maintenance of motility, plasma membrane integrity, acrosomal integrity, T-AOC activity and MDA content. These results may suggest the possibility that the proper addition of taurine to the semen extender improves the swine production system using artificial insemination by the suppressing of sperm damage and subsequent dysfunction during liquid preservation.

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Section: Figuresupporting

confidence: 56%

“…Low enzyme activity membranes and acrosomes cause loss of function and integrity. On the other hand, the negative effects of the highest concentration of taurine might be caused by high osmotic pressure, as described previously (Stephens et al 2013).…”

Section: Discussionmentioning

confidence: 53%

Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Zhang

Qian

et al. 2017

Animal Science Journal

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“…In another study, the effect of pentoxifylline on increasing horse seminal lifetime has been shown 25. In another study, rate of sperms with normal morphology had increased with pentoxifylline at 400 mg twice a day and acrosomal reaction has also increased 26…”

Section: Discussionmentioning

confidence: 94%

Study of pentoxifylline effects on motility and viability of spermatozoa from infertile asthenozoospermic males

et al. 2016

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Background:The quality of semen is one of the major parameters in male infertility. Pentoxifylline, a methylxanthine derivative, is an agent primarily used in the treatment of intermittent claudication and other vascular disorders. Studies have shown that pentoxifylline enhances the quality and quantity of sperms. In this study, we have investigated the in vitro effects of pentoxifylline on viability and motility of spermatozoa in samples of infertile oligoasthenozoospermic males.Materials and Methods:In this observer-blinded clinical trial, semen samples of 25 infertile oligoasthenozoospermic males were collected in Alzahra Educational Medical Center of Tabriz University of Medical Sciences from August 2010 to August 2012. After the isolation of spermatozoa by the swim-up method, they were randomized into four groups in ISM1 environment: The controls treated normally: Group 1 treated by pentoxifylline at a dose of 50 μg/ml, Group 2 treated by pentoxifylline at a dose of 100 μg/ml, and Group 3 treated by pentoxifylline at a dose of 200 μg/ml. Sperm viability and motility were compared among the groups on 45 min, 24 h, 36 h, and 48 h intervals.Results:Mean percentages of live sperms were 98.40%, 51.40%, 20.60%, and 6.00% in control group and 98.40%, 69.20%, 38.60%, and 14.60% in Group 3 on the mentioned intervals, respectively. This mean percentage decrease of live sperms was significantly lower in Group 3 comparing with that of other groups (P = 0.01). Mean percentages of motile sperms were 54%, 8.40%, 2.80%, and 0% in control group; and 54%, 16%, 4.80%, and 1.40% in Group 3 on the mentioned intervals, respectively. There was not a significant difference between the four groups in this regard (P = 0.19).Conclusion:Pentoxifylline can enhance the viability of sperm of infertile oligoasthenozoospermic males with no significant effect on its motility.

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“…The development of assisted reproductive techniques, including artificial insemination (AI) and in vitro fertilization (IVF), are considered the most important means for improving reproductive efficiency and productivity in ruminants (Camargo et al., ; Srivastava & Kumar, ). However, these methods require improving spermatozoa physical characteristics by a variety of substances such as Ca 2+ ionophore A23187, heparin (El‐Shahat, Taysser, Badr, & Zaki, ), caffeine (Barakat, Danfour, Galewan, & Dkhil, ), cAMP (Singh & Raina, ), pentoxifylline and taurine (Stephens, Brooks, Cheng, Porr, & Splan, ). These treatments have been reported to affect integrity of sperm membrane, stimulate sperm motility, improve fertilization and initiate acrosome reaction (Zietoun & Al‐Damegh, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen

El-Hamid

2018

Reprod Domestic Animals

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Two experiments were carried out to determine the efficiency of supplementation of ram semen extender with caffeine on chilled storage and frozen capacity of spermatozoa. In the first experiment, eighty ejaculates were collected by an artificial vagina from five adult Barki rams, aged 2-3 years and weighted 45.0 ± 2.0 kg throughout the experimental period (January to February 2017). The ejaculates were pooled and diluted (1:10) with tris-citric egg yolk extender and were split into five groups. Group 1 served as control, whereas groups 2-5 were supplemented with 0.1, 0.2, 0.3 and 0.4 mM caffeine. All diluted semen specimens were evaluated for physical characteristics immediately after dilution (T ) and throughout preservation period of 48 hr at 4°C. Simultaneously, oxidative stress and indices such as total antioxidant capacity (TAC), malondialdehyde concentrations (MDA) and alkaline transaminase (AKP) concentrations and value of resazurin reduction test (RRT) were determined. In the second experiment, the raw pooled ejaculates were diluted (1:10) with glycerolated tris-citric egg yolk extender, receiving the previously mentioned caffeine levels. The post-thaw assessment of cryopreserved spermatozoa, in all groups, was conducted by a computer-assisted sperm analysis (CASA) system. The results revealed that adding caffeine to ram semen extender at low (0.1 mM) or medium (0.2 mM) levels had positive impact on both physical characteristics of ram sperm and the enzymatic activities compared to the other semen groups. Caffeine supplementation also enhanced post-thaw sperm dynamics, which implies its potential as an exogenous antioxidant supplement.

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Effects of Pentoxifylline, Caffeine, and Taurine on Post-Thaw Motility and Longevity of Equine Frozen Semen

Cited by 35 publications

References 24 publications

Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Supplemental effect of different levels of taurine in Modena on boar semen quality during liquid preservation at 17°C

Study of pentoxifylline effects on motility and viability of spermatozoa from infertile asthenozoospermic males

Effect of adding different levels of caffeine in the extender on some biochemical constituents, enzymatic activities and physical characteristics of chilled and frozen ram semen

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