Effects of periodate and chondroitin 4-sulfate on proteoglycan stabilization of ostrich pericardium. Inhibition of calcification in subcutaneous implants in rats

Arenaz, B.; Maestro, M. Martín; Fernández, P.; Turnay, Javier; Olmo, Nieves; Senén, Jesús; Mur, Javier Gil; Lizarbe, M.A.; Jorge-Herrero, Eduardo

doi:10.1016/j.biomaterials.2003.09.105

Cited by 12 publications

(9 citation statements)

References 33 publications

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“…This GAGs dissociation caused the change in the stress relaxation properties of the pericardial tissue, especially after RAS-ATE treatment. The highly hydrated and negatively charged polysaccharides present in collagenous tissues appear to play an important role in modulating physiological biomechanical behavior and in the tissue calcification [36,[39][40]. In this study, the significant role of GAG/PG in viscoelastic behavior of decellularized pericardial tissue was confirmed by stress relaxation analysis.…”

Section: Discussionsupporting

confidence: 54%

Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content

et al. 2011

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Keywords:Tissue decellularization Pericardium Glycosaminoglycans Tensile properties Detergents Bovine pericardium is a collagenous tissue commonly used as a natural biomaterial in the fabrication of cardiovascular devices. For tissue engineering purposes, this xenogeneic biomaterial must be decellularized to remove cellular antigens. With this in mind, three decellularization protocols were compared in terms of their effectiveness to extract cellular materials, their effect on glycosaminoglycan (GAG) content and, finally, their effect on tensile biomechanical behavior. The tissue decellularization was achieved by treatment with t-octyl phenoxy polyethoxy ethanol (Triton X-100), tridecyl polyethoxy ethanol (ATE) and alkaline treatment and subsequent treatment with nucleases (DNase/RNase). The quantified residual DNA content (3.0 ± 0.4%, 4.4 ± 0.6% and 5.6 ± 0.7% for Triton X-100, ATE and alkaline treatment, respectively) and the absence of nuclear structures (hematoxylin and eosin staining) were indicators of effective cell removal. In the same way, it was found that the native tissue GAG content decreased to 61.6 ± 0.6%, 62.7 ± 1.1% and 88.6 ±0.2% for Triton X-100, ATE and alkaline treatment, respectively. In addition, an alteration in the tissue stress relaxation characteristics was observed after alkaline treatment. We can conclude that the three decellularization agents preserved the collagen structural network, anisotropy and the tensile modulus, tensile strength and maximum strain at failure of native tissue.

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Section: Discussionsupporting

confidence: 54%

Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content

et al. 2011

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“…Covalent binding of GAGs, such as heparin and chondroitin sulfate, were shown to reduce calcification of GF bioprostheses in experimental models [5,14]. Hyaluronic acid (HA) is a simple and linear glycosaminoglycan (GAG) composed of alternating disaccharide units of Dglucuronic acid and N-acetyl-D-glucosamine with ȕ(1-4) interglycosidic linkage.…”

Section: Introductionmentioning

confidence: 99%

Combined anticalcification treatment of bovine pericardium with decellularization and hyaluronic acid derivative

Zhu

Jin

Wang

et al. 2014

Bio-Medical Materials and Engineering

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The objective of this work was to evaluate the effect of decellularization and hyaluronic acid derivative on the improvement of anticalcification of glutaraldehyde fixed bovine pericardium (GFBP) using a rat subcutaneous implantation model A cell extraction process was employed to remove the cells and cellular components from bovine pericardium (BP), leaving a framework of largely insoluble collagen. Then acellular BP was cross-linked by glutaraldehyde solution and treated with hyaluronic acid derivative (HA-ADH) which was obtained by coupling adipic dihydrazide (ADH) on-COOH of hyaluronic acid (HA). The results of in vivo calcification tests showed that the calcium content was decreased dramatically by decellularization alone (from 28.07 ± 18.87 to 2.44 ± 0.55ȝg Ca/mg dry tissue after 8 weeks' implantation), and even less concentration was shown by the combination of HA derivative treatment and decellularization (GFaBP-HA group) (0.25±0.08ȝg Ca/mg dry tissue after 8 weeks' implantation). In addition, GFaBP-HA group not only presented a lower degree of calcification, but also showed lower ratios of Ca/P molar, which corresponded to amorphous calcium phosphates. The obtained results indicated that GFaBP-HA was a potential candidate for the manufacture of anticalcification bioprostheses.

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“…These groups are negatively charged and they are thought to attract calcium ions, promoting the formation of hydroxyapatite; however, their role is controversial. Highlynegatively charged compounds containing sulfonates and sulfates, such as heparin, carrageenan and chondroitin sulfate, seem to act as efficient anti-calcifying agents [29,30,33]. The anionic nature of the sulfonate and sulfate groups, present in both modified chitosan and carrageenan, may cause a local decrease in pH, which may mediate the dissolution of the deposits of calcium compounds formed, thus…”

Section: Accepted Manuscriptmentioning

confidence: 97%

In vitro evaluation of anti-calcification and anti-coagulation on sulfonated chitosan and carrageenan surfaces

Campelo

Lima

Rebêlo

et al. 2016

Materials Science and Engineering: C

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Effects of periodate and chondroitin 4-sulfate on proteoglycan stabilization of ostrich pericardium. Inhibition of calcification in subcutaneous implants in rats

Cited by 12 publications

References 33 publications

Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content

Decellularization of pericardial tissue and its impact on tensile viscoelasticity and glycosaminoglycan content

Combined anticalcification treatment of bovine pericardium with decellularization and hyaluronic acid derivative

In vitro evaluation of anti-calcification and anti-coagulation on sulfonated chitosan and carrageenan surfaces

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