Effects of Peroxide-Based Contact Lens–Disinfecting Systems on Human Corneal Epithelial Cells In Vitro

Konynenbelt, Benjamin J; Mlnarik, Daniel S; Ubels, John L.

doi:10.1097/icl.0b013e3182182d02

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Corneal opacity, which is a consequence of corneal endothelial damage due to the penetration of administrated H 2 O 2 , , was alleviated for the mice wearing 50 mM CeNP-CLs, and this was noticeable 3 d after the H 2 O 2 exposure (Figures d, e). Corneal limbal epithelium is an important component in corneal epithelial wound healing owing to the presence of stem cells, and it is susceptible to oxidative stress caused by H 2 O 2 . − At day 11 after H 2 O 2 exposure, the mice were sacrificed, eyeballs were retrieved, and its cross sections were observed (Figures f–h), revealing that corneal epithelial defects were more severe for the group wearing 0 mM CeNP-CLs than that for the group wearing 50 mM CeNP-CLs. These findings suggest that enough neutralization of H 2 O 2 in the presence of CeNPs-CLs can reduce corneal epithelial and endothelial damage.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Contact Lens for Scavenging Excessive Reactive Oxygen Species on the Ocular Surface

2020

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Excessive reactive oxygen species (ROS) play a significant role in the pathogenesis of many eye diseases. Controlling oxidative stress by reducing the amount of ROS is a potential therapeutic strategy for the prevention and treatment of eye diseases, particularly ocular surface diseases. Ceria nanoparticles (CeNPs) have been investigated owing to their efficient ROS-scavenging properties. To overcome the disadvantages of eyedrop administration due to rapid elimination on the surface of the eye and to retain the intrinsic properties of contact lenses, we developed an ROS-scavenging water-soluble CeNP-embedded contact lens (CeNP-CL) for the prevention of ocular surface diseases. The intrinsic ROS-scavenging property of the CeNPs, which mimicked the activities of superoxide dismutase and catalase, was incorporated into polyhydroxyethyl methacrylate-based contact lenses. The CeNP-CL exhibited high transparency and physical properties comparable to those of a commercial contact lens, along with excellent extracellular ROS-scavenging properties. The viabilities of human conjunctival epithelial cells and human meibomian gland epithelial cells were significantly enhanced in the presence of CeNP-CLs, even in media with high H 2 O 2 contents (100 and 500 μM). Additionally, the wearing of CeNP-CLs on the eyes had a protective effect in a mouse model when 3% H 2 O 2 eyedrops were administered. These results indicate the salvaging effect of the CeNP-CL in a high-ROS environment on the ocular surface, which may be helpful for the treatment of ocular surface diseases.

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Section: Resultsmentioning

confidence: 99%

Therapeutic Contact Lens for Scavenging Excessive Reactive Oxygen Species on the Ocular Surface

2020

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“…Immortalized human corneal limbal epithelial (HCLE) cells were used for the cytotoxicity assay; that cell line has previously been used to evaluate the biomaterial toxicity of ocular devices [22] and chemicals intended for ocular exposure [23]. Cytotoxicity was assessed by incubating HCLE cells in media that contained a Transwell ® insert, which held a commercial methafilcon contact lens (Kontur 55, Kontur Kontact Lens Company), a CL 85:15, 45 , or vehicle (media only, no contact lens).…”

Section: Resultsmentioning

confidence: 99%

In vivo performance of a drug-eluting contact lens to treat glaucoma for a month

Ciolino¹,

Ştefănescu²,

Ross³

et al. 2014

Biomaterials

167

122

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For nearly half a century, contact lenses have been proposed as a means of ocular drug delivery, but achieving controlled drug release has been a significant challenge. We have developed a drug-eluting contact lens designed for prolonged delivery of latanoprost for the treatment of glaucoma, the leading cause of irreversible blindness worldwide. Latanoprost-eluting contact lenses were created by encapsulating latanoprost–poly(lactic-co-glycolic acid) films in methafilcon by ultraviolet light polymerization. In vitro and in vivo studies showed an early burst of drug release followed by sustained release for one month. Contact lenses containing thicker drug–polymer films demonstrated released a greater amount of drug after the initial burst. In vivo, single contact lenses were able to achieve, for at least one month, latanoprost concentrations in the aqueous humor that were comparable to those achieved with topical latanoprost solution, the current first-line treatment for glaucoma. The lenses appeared safe in cell culture and animal studies. This contact lens design can potentially be used as a treatment for glaucoma and as a platform for other ocular drug delivery applications.

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“…In contradistinction, barrier function measurement offers an objective and quantitative in vivo measure, in the contact lens-wearing patient, of corneal health. In that regard, barrier function measurement is widely used to examine toxic phenomena, for example, in tissue culture 6 and in vivo. 7 3.…”

Section: Reference 6 Of Bloomenstein Et Almentioning

confidence: 99%

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Nguyen

Hall

et al. 2013

Cornea

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Effects of Peroxide-Based Contact Lens–Disinfecting Systems on Human Corneal Epithelial Cells In Vitro

Cited by 5 publications

References 19 publications

Therapeutic Contact Lens for Scavenging Excessive Reactive Oxygen Species on the Ocular Surface

Therapeutic Contact Lens for Scavenging Excessive Reactive Oxygen Species on the Ocular Surface

In vivo performance of a drug-eluting contact lens to treat glaucoma for a month

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