Effects of Pesticides on the Diversity of Endophytic Fungi in Tea Plants

Win, Phyu Mar; Matsumura, Emi; Fukuda, Kenji

doi:10.1007/s00248-020-01675-7

Cited by 14 publications

(10 citation statements)

References 44 publications

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“…Our results reveal that the endophytic activity of B. bassiana Bb-18 and M. anisopliae Ma-30 on roots of common bean was suppressed by the effect of Tebuconazole, Mancozeb and Glufosinate. Similar results were obtained by Win et al (2021) who demonstrated that the endophytic colonization of fungi in different tea plant tissues obtained from a plantation treated with agrochemicals was lower than that in untreated plants. These The effects of other synthetic insecticides were tested on two M. anisopliae strains (MA-7 and MA-K) and the results revealed that a dosage of Fipronil over 40 and 1.6 ppm produced a lower number of conidia than the control, respectively.…”

Section: Effect Of Synthetic Pesticides On the Endophytic Activity Of...supporting

confidence: 87%

EFFECT OF SYNTHETIC PESTICIDES ON CONIDIAL GERMINATION AND ENDOPHYTIC ACTIVITY OF Beauveria bassiana AND Metarhizium anisopliae IN COMMON BEAN PLANTS

González¹,

Taibo²,

D’Alessandro³

2022

Chil. j. agric. anim. sci.

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Common bean (Phaseolus vulgaris L.) is an important legume crop worldwide. Endophytic fungi have proven effective as biocontrol agents in several crops, but they can be affected by pesticides. The aim of this study was to evaluate the effect of synthetic pesticides on the conidial germination and endophytic activity of Beauveria bassiana and Metarhizium anisopliae in P. vulgaris. Three fungicides (Mancozeb, Tebuconazole and Zinc Ethylene-bis), three insecticides (Abamectin, Diazinon and Spirotetramat) and one herbicide (Glufosinate) were evaluated. Each pesticide was applied at the application rate recommended by the manufacturer and also using one-half of the application rate on the commercial strains B. bassiana Bb-18 and M. anisopliae Ma-30. Tebuconazole, Mancozeb and Glufosinate totally inhibited conidial germination. Spirotetramat and Diazinon resulted in a less pronounced negative effect on B. bassiana and M. anisopliae germination. However, Spirotetramat has the lowest impact on the entomopathogenic fungi, allowing for 86 and 74% germination of B. bassiana and M. anisopliae, respectively. Tebuconazole and Mancozeb affected the endophytic activity of the entomopathogenic fungi studied. At one-half of the recommended concentration, Spirotetramat and Zinc Ethylene-bis allowed for the total colonization of B. bassiana in roots of common bean, whereas M. anisopliae reached a colonization of 75%. These results indicate that Spirotetramat, Zinc Ethylene-bis and Diazinon can be used in combination with B. bassiana Ba-18 and M. anisopliae Ma-30. However, Tebuconazole, Mancozeb and Glufosinate should not be combined with these entomopathogenic fungi in Integrated Pest Management programs.

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Section: Effect Of Synthetic Pesticides On the Endophytic Activity Of...supporting

confidence: 87%

EFFECT OF SYNTHETIC PESTICIDES ON CONIDIAL GERMINATION AND ENDOPHYTIC ACTIVITY OF Beauveria bassiana AND Metarhizium anisopliae IN COMMON BEAN PLANTS

González¹,

Taibo²,

D’Alessandro³

2022

Chil. j. agric. anim. sci.

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“…Tea (Camellia sinensis (L.) Kuntze) is a widely cultivated important cash crop throughout the tropical and subtropical regions, including Asia and Africa. It is the most popular beverage in the world and is reported to have a wide range of beneficial physiological and medicinal properties (Namita et al, 2012;Win et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Endophytic fungi are a diverse group of microorganisms that reside asymptomatically in the healthy tissues of the host. They are well known for their bioactive secondary metabolites that may support the host in defense and survival against pathogenic and insect attacks, stress tolerance and disease resistance (Strobel and Daisy, 2003;Rodriguez et al, 2009;Rabha et al, 2014;Win et al, 2021). Many fungal endophytes are associated with the tea plant and the pathogens of the tea plant have the potential to grow as endophytes or act as latent pathogens during the initial growth of the plant (Fang et al, 2013;Xie et al, 2020;Thambugala et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol potential of endophytic fungi in tea (Camellia sinensis (L.) Kuntze) leaves against selected fungal phytopathogens

Thambugala¹,

Daranagama²,

Kannangara³

2022

MJM

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Aims: Endophytic fungi are a diverse group of microorganisms that stay asymptomatically in the healthy tissues of the host. Many fungal endophytes are associated with the tea plant (Camellia sinensis) and the pathogens of the tea plant have the potential to grow as endophytes or act as latent pathogens during the initial growth of the plant. The present study aimed at screening tea endophytic fungi with the potential for control of a few critical phytopathogens, Fusarium sp., Lasiodiplodia theobromae, Pestalotiopsis sp. and Sclerotinia sclerotiorum while evaluating the efficiency of growth inhibition of these phytopathogens by the endophytic fungal isolates using in vitro assays. Methodology and results: Five endophytic fungal strains; Colletotrichum gloeosporioides, C. siamense, Daldinia eschscholtzii, Pseudopestalotiopsis chinensis and Phyllosticta capitalensis isolated from leaves of Camellia sinensis in Sri Lanka were evaluated for growth inhibition against plant pathogens; Fusarium sp., L. theobromae, Pestalotiopsis sp. and S. sclerotiorum using the dual culture assay and volatile compound-mediated inhibition assay. All the fungal endophytes used in this study exhibited antifungal activity against Fusarium sp., Pestalotiopsis sp. and S. sclerotiorum in the dual culture assay on PDA. Daldinia eschscholtzii (67.30%) and C. gloeosporioides (61.54%) showed strong antagonistic activity against S. sclerotiorum, while P. capitalensis (42.30%) demonstrated moderate activity. All the endophytic fungal strains showed moderate antifungal activities against Fusarium sp. The percentage growth inhibitions of Pestalotiopsis sp. by all the endophytic isolates tested were below 25.00%. In the volatile compound-mediated inhibition assay, none of the endophytic isolates showed visible inhibition against the phytopathogens used. Conclusion, significance and impact of study:In this study, the fungal endophytes which showed potential antagonistic activity against the tested phytopathogens can be used to develop commercialized products of fungal biocontrol agents (BCAs) for controlling plant fungal diseases.

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“…Very doi :10.57023/JMycR. 60.4.2022.601 Pesticides affecting diversity of mycoflora recently the effects of pesticides on the diversity of endophytic fungi in tea plants caused by Colletotrichum camelliae were studied by Win et al 2021.…”

Section: Introductionmentioning

confidence: 99%

Studies on the effect of pesticides on the mycoflora diversity in the agricultural environments

Uddin¹

2022

JMycR

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Plant leaves are the natural habitat for growth of many microorganisms like fungi, bacteria, actinomycetes etc. Various chemicals are sprayed time to time over the leaves of economically important crops to manage the insect pests, pathogenic fungi, nematodes, pathogenic bacteria etc. Three chemicals were tested in laboratory condition over the fungal strains isolated from phylloplane. Cildon (85% Phosphamidon) showed various degrees of inhibition on Aspergillus ochraceus, Curvularia geniculata, Alternaria solani, A. brassicae, A. tenuissima, A. tenuis, Nigrospora sphaerica and on other fungi tested by cup assay method. BHC caused total inhibition over Curvularia lunata, C. geniculata, Penicillium, Stachybotrys atra, T. lignorum, Cladosporium herbarum, Rhizopus nigricans and also over pathogenic types like Drechslera, Alternaria solani, A. brassicae, A. tenuissima, A. tenuis etc. Although a number of fungi were inhibited totally with Bavistin, a fungicide, 12 fungi were found to grow normally inc luding plant pathogenic Alternaria spp. exc ept A. tenuis , Drechs lera, Helminthosporium sativum and the saprobic Curvularia lunata, C. pallescens, S. atra, E. purpurascens, Brachysporium and Nigrospora sphaerica. Similar results were also obtained in dry weight method with Cildon and Bavistin. BHC caused toxicity to the whole culture medium showing nil growth in almost all fungi under experiment.

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Effects of Pesticides on the Diversity of Endophytic Fungi in Tea Plants

Cited by 14 publications

References 44 publications

EFFECT OF SYNTHETIC PESTICIDES ON CONIDIAL GERMINATION AND ENDOPHYTIC ACTIVITY OF Beauveria bassiana AND Metarhizium anisopliae IN COMMON BEAN PLANTS

EFFECT OF SYNTHETIC PESTICIDES ON CONIDIAL GERMINATION AND ENDOPHYTIC ACTIVITY OF Beauveria bassiana AND Metarhizium anisopliae IN COMMON BEAN PLANTS

Biocontrol potential of endophytic fungi in tea (Camellia sinensis (L.) Kuntze) leaves against selected fungal phytopathogens

Studies on the effect of pesticides on the mycoflora diversity in the agricultural environments

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