Effects of pH and dissolved CO2 level on simultaneous production of 2,3-butanediol and succinic acid using Klebsiella pneumoniae

Cheng, Ke-Ke; Wu, Jing; Wang, Genyu; Li, Wenying; Feng, Jie; Zhang, Jianan

doi:10.1016/j.biortech.2012.08.100

Cited by 25 publications

(15 citation statements)

References 15 publications

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“…The effects of different kinds and concentrations of carbonate salts on the simultaneous production of 2,3‐BDO and SA have been previously investigated. The promoting effects of sodium bicarbonate on the combined production of 2,3‐BDO and SA were observed to be more effective than calcium carbonate or magnesium carbonate . The mechanism by which the SA pathway is enhanced during 2,3‐BDO fermentation in E. cloacae was investigated (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…The concentration of SA that is produced can be greatly improved under suitable conditions during 2,3‐BDO fermentation. CO 2 is essential for the biosynthesis of SA, and the increase in SA production by the addition of CO 2 or carbonate to the fermentation medium has been demonstrated . Different bicarbonate salts, such as sodium bicarbonate, calcium carbonate and magnesium carbonate, have been used as a CO 2 source during the combined production of 2,3‐BDO and SA from glucose using E. cloacae .…”

Section: Introductionmentioning

confidence: 99%

“…Different bicarbonate salts, such as sodium bicarbonate, calcium carbonate and magnesium carbonate, have been used as a CO 2 source during the combined production of 2,3‐BDO and SA from glucose using E. cloacae . The effects of sodium bicarbonate on the combined production of 2,3‐BDO and SA were observed to be more effective than calcium carbonate and magnesium carbonate . However, the mechanisms by which the SA pathway is enhanced during 2,3‐BDO fermentation in E. cloacae have not been well‐studied, and the addition of sodium bicarbonate has also not been optimized.…”

Section: Introductionmentioning

confidence: 99%

“…CO 2 is essential for the biosynthesis of SA, and the increase in SA production by the addition of CO 2 or carbonate to the fermentation medium has been demonstrated. 11,12 Different bicarbonate salts, such as sodium bicarbonate, calcium carbonate and magnesium carbonate, have been used as a CO 2 source during the combined production of 2,3-BDO and SA from glucose using E. cloacae. The effects of sodium bicarbonate on the combined production of 2,3-BDO and SA were observed to be more effective than calcium carbonate and magnesium carbonate.…”

Section: Introductionmentioning

confidence: 99%

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Co‐production of 2,3‐BDO and succinic acid using xylose by Enterobacter cloacae

Liu

Yan

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND 2,3‐Butanediol and succinic acid are staple chemicals that are widely used in the chemical industry. In this study, a new strategy for the simultaneous fermentation of 2,3‐butanediol and succinic acid by Enterobacter cloacae using xylose was developed. The mechanism by which the succinic acid pathway was enhanced during 2,3‐butanediol fermentation in E. cloacae was studied. In addition, the sodium bicarbonate feeding mode and time, pH, and aeration rate were optimized. Fed‐batch fermentation was performed under the optimal conditions using industrial xylose as a raw material. RESULTS The succinic acid pathway was enhanced by the addition of sodium bicarbonate during 2,3‐butanediol fermentation. Interestingly, a simple increase of the initial pH had no effect on the production of succinic acid. The sodium bicarbonate feeding mode and time, pH and aeration rate were optimized. Under the optimum conditions, including a sodium bicarbonate feeding mode of 2 and time of 12 h, a pH of 6.5 and an aeration rate of 0.4 vvm, a maximum of 40.67 g L‐1 of 2,3‐butanediol and 21.79 g L‐1 of succinic acid were obtained after a 72 h fed‐batch fermentation when xylose was used as raw material, with a total 2,3‐butanediol + succinic acid yield of 0.69 mol mol‐1 xylose. CONCLUSION 2,3‐Butanediol and succinic acid were produced in a single fermentation step using E. cloacae. The production of 2,3‐butanediol and succinic acid was enhanced by controlling the sodium bicarbonate feeding mode and time, pH, and aeration rate. This type of fermentation provides a promising means of lowering the cost of production of these chemicals by reducing the fermentation operating time and fermentation equipment maintenance. © 2017 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Co‐production of 2,3‐BDO and succinic acid using xylose by Enterobacter cloacae

Liu

Yan

et al. 2017

J of Chemical Tech & Biotech

Self Cite

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“…Due to depleting of oil reserves, rising prices and environmental pollution, succinic acid production through fermentation using low cost substrates such as lignocellulosic biomass has attracted more and more attention in recent years [151] [152], pinewood [156], and Cannabis sativaL [157]. Microorganisms that naturally produce the succinic acid are Actinobacillus succinogenes [153] [156] [157], Anaerobiospirillum succiniciproducens [156], Klebsislla pneumonia [158], and Mannheimia succiniciproducens [155] etc. Along with these microbial strains, genetically engineered E. Coli.…”

Section: Succinic Acidmentioning

confidence: 99%

Biotechnological Transformation of Lignocellulosic Biomass in to Industrial Products: An Overview

Kumar

Gautam

Dutt

2016

ABB

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Lignocellulose-a major component of biomass available on earth is a renewable and abundantly available with great potential for bioconversion to value-added bio-products. The review aims at physio-chemical features of lignocellulosic biomass and composition of different lignocellulosic materials. This work is an overview about the conversion of lignocellulosic biomass into bio-energy products such as bio-ethanol, 1-butanol, bio-methane, bio-hydrogen, organic acids including citric acid, succinic acid and lactic acid, microbial polysaccharides, single cell protein and xylitol. The biotechnological aspect of bio-transformation of lignocelluloses research and its future prospects are also discussed.

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Biotechnological route for sustainable succinate production utilizing oil palm frond and kenaf as potential carbon sources

Luthfi

Manaf

Illias

et al. 2017

Appl Microbiol Biotechnol

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Due to the world's dwindling energy supplies, greater thrust has been placed on the utilization of renewable resources for global succinate production. Exploration of such biotechnological route could be seen as an act of counterbalance to the continued fossil fuel dominance. Malaysia being a tropical country stands out among many other nations for its plenty of resources in the form of lignocellulosic biomass. To date, oil palm frond (OPF) contributes to the largest fraction of agricultural residues in Malaysia, while kenaf, a newly introduced fiber crop with relatively high growth rate, holds great potential for developing sustainable succinate production, apart from OPF. Utilization of non-food, inexhaustible, and low-cost derived biomass in the form of OPF and kenaf for bio-based succinate production remains largely untapped. Owing to the richness of carbohydrates in OPF and kenaf, bio-succinate commercialization using these sources appears as an attractive proposition for future sustainable developments. The aim of this paper was to review some research efforts in developing a biorefinery system based on OPF and kenaf as processing inputs. It presents the importance of the current progress in bio-succinate commercialization, in addition to describing the potential use of different succinate production hosts and various pretreatments-saccharifications under development for OPF and kenaf. Evaluations on the feasibility of OPF and kenaf as fermentation substrates are also discussed.

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Effects of pH and dissolved CO2 level on simultaneous production of 2,3-butanediol and succinic acid using Klebsiella pneumoniae

Cited by 25 publications

References 15 publications

Co‐production of 2,3‐BDO and succinic acid using xylose by Enterobacter cloacae

Co‐production of 2,3‐BDO and succinic acid using xylose by Enterobacter cloacae

Biotechnological Transformation of Lignocellulosic Biomass in to Industrial Products: An Overview

Biotechnological route for sustainable succinate production utilizing oil palm frond and kenaf as potential carbon sources

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