Effects of pH on the properties of normal and 5-fluorouracil-containing tRNAs

Inhibition of transfer RNA function by replacement of uridine and uridine-derived nucleosides with 5-fluorouridine

“…There was a small increase in the rate of aminoacylation between pH 6.5 and 7.5, but the rate decreased when the pH was increased further to 8.5. Similar effects of pH on the aminoacylation of [FUrdJ-tRNA with lysine were recently reported by Moore and Kaiser (1977). The pKa of 5-fluorouracil, 8.1-8.3 in poly(FU) (Szer and Shugar, 1963;Massoulie et al, 1966), is considerably lower than the pKa of uracil, 9.6-9.8 in poly(U).…”