Effects of Phenanthrene and Pyrene on Cytogenetic Stability of Human Dermal Fibroblasts Using Alkaline Comet Assay Technique

Abstract

In the present study, the influence of phenanthrene and pyrene on cytogenetic stability of human dermal fibroblasts using alkaline comet assay was evaluated. The cells were isolated from foreskin samples obtained from healthy male infants and in low passages (P 1-3) were exposed to various concentrations (0.0900, 0.0625, 0.0320, and 0.0066 ppm) of phenanthrene and pyrene. Then, alkaline comet assay was performed to investigate the influence of these compounds on DNA damage and cytogenetic stability of human dermal fibroblasts. In the present work H 2 O 2 treatment was used as a positive control of comet assay to create DNA damages. The analysis of alkaline comet assay parameters by CaspLab software showed the tail DNA% in high concentration (0.0900 ppm) of phenanthrene and pyrene in the exposed cells got increased as compared to normal cells, while head DNA% decreased. Also, similar to positive control (H 2 O 2 ), DNA damage with long tail comet was observed in high concentration of these compounds as compared to normal cells. The comparison of comet assay parameters specially head DNA% and tail DNA% between each concentrations of phenanthrene and pyrene with other groups (healthy cells and H 2 O 2 treatment) by ANOVA and post hoc Tukey test showed that these parameters were more significantly different (p \ 0.05). These results indicated that phenanthrene and pyrene even in low dosage are dangerous and can increase the DNA damage and affect on cytogenetic stability of dermal fibroblasts. These findings suggested that phenanthrene and pyrene could increase the related diseases and risk of skin cancer in exposed individuals.

Investigating the expression pattern of the angiopoietin-Tie system in ALL and its correlation with baseline characteristics

“…[ 23 ], the expression of this gene in BM samples from AML (acute myeloid leukemia) patients did not show a statistically significant difference compared to normal samples. Conversely, other studies have shown Ang1 upregulation in AML patients, either in BM or PB samples [ 24 - 29 ]. In a study by Cheng et al .…”

The Expression of P53, MDM2, c-myc, and P14ARF Genes in Newly Diagnosed Acute Lymphoblastic Leukemia Patients

“…The expression of P53 gene is associated with the cell cycle of myeloblasts and the cellular internal and external messages in acute myeloid leukemia (AML) [14]. As well as cytogenetic instability and mutation in proto-oncogenes due to chemical toxic materials [15,16] which can affect P53 expression [17]. It has been thought that the percentage of lymphoblasts is linked with the status of the cell cycle in ALL patients.…”

Application of Genetically Engineered Dioxygenase Producing Pseudomonas putida on Decomposition of Oil from Spiked Soil

“…PAHs are ubiquitous environmental contaminants and enter the environment through various ways that are found in soil, air, water, and food sources (16,17). PAH compounds are known as human carcinogens that spread during incomplete combustion of organic matter by industrial and other human activities (e.g., combustion of urban sewage, garbage, natural gas, vehicle traffic, aluminium smelters, tobacco smoking, cooking, power plants chemical, asphalt works, petrochemical, and oil industries) as well as natural processes like burning coal and wood or forest fires (18)(19)(20)(21). Natural crude oil and kerosene contain significant amounts of PAHs (22).…”