Effects of phosphorylation on the activity of glycogen phosphorylase in mutton during incubation at 4 °C in vitro

Bai, Yuqiang; Li, Xin; Zhang, Dequan; Chen, Li; Hou, Chengli; Zheng, Xiaochun; Ren, Chi

doi:10.1016/j.foodchem.2020.126162

Cited by 8 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 Phosphorylation level of desmin, actin, and myosin light chain was assessed by Zn 2+ -Phos-tag SDS-PAGE (Wako, Osaka, Japan) and Western blot. 18 The 5 mmol/L Phos-tag and 10 mmol/L ZnCl 2 were contained in an 8% separating gel. The electrophoresis of each Zn 2+ -Phos-tag gel was run at 30 mA for 1 h. The Western blot was performed at 250 mA for 2.5 h to transfer proteins from gels to PVDF membranes.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Protein Phosphorylation Induced by Pyruvate Kinase M2 Inhibited Myofibrillar Protein Degradation in Post-Mortem Muscle

Ren,

Song,

Dong

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Myofibrillar protein degradation is primarily related to meat tenderness through protein phosphorylation regulation. Pyruvate kinase M2 (PKM2), a glycolytic rate-limiting enzyme, is also regarded as a protein kinase to catalyze phosphorylation. The objective of this study was to investigate the relationship between myofibrillar protein degradation and phosphorylation induced by PKM2. Myofibrillar proteins were incubated with PKM2 at 4, 25, and 37 °C. The global phosphorylation level of myofibrillar proteins in the PKM2 group was significantly increased, but it was sensitive to temperature (P < 0.05). Compared with 4 and 25 °C, PKM2 significantly increased the myofibrillar protein phosphorylation level from 0.5 to 6 h at 37 °C (P < 0.05). In addition, the degradation of desmin and actin was inhibited after they were phosphorylated by PKM2 when incubated at 37 °C. These results demonstrate that phosphorylation of myofibrillar proteins catalyzed by PKM2 inhibited protein degradation and provided a possible pathway for meat tenderization through glycolytic enzyme regulation.

show abstract

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Phosphorylation level of desmin, actin, and myosin light chain was assessed by Zn 2+ -Phos-tag SDS-PAGE (Wako, Osaka, Japan) and Western blot . The 5 mmol/L Phos-tag and 10 mmol/L ZnCl 2 were contained in an 8% separating gel.…”

Section: Methodsmentioning

confidence: 99%

Protein Phosphorylation Induced by Pyruvate Kinase M2 Inhibited Myofibrillar Protein Degradation in Post-Mortem Muscle

Ren,

Song,

Dong

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Glycogen phosphorylase is one of the most important enzymes involved in the tenderness of meat and may affect the quality of it [48]. Therefore, genetic variations could not only affect what is related to stress or welfare, but the stress conditions linked to the end of the productive stage will determine the characteristics of the final product, in this case, the meat [48,49].…”

Section: Chromosomes and Candidate Genesmentioning

confidence: 99%

Identification of Candidate Genes and Pathways Linked to the Temperament Trait in Sheep

Romaniuk,

Vera,

Peraza

et al. 2024

Genes

View full text Add to dashboard Cite

Temperament can be defined as the emotional variability among animals of the same species in response to the same stimulus, grouping animals by their reactivity as nervous, intermediate, or calm. Our goal was to identify genomic regions with the temperament phenotype measured by the Isolation Box Test (IBT) by single-step genome-wide association studies (ssGWAS). The database consisted of 4317 animals with temperament records, and 1697 genotyped animals with 38,268 effective Single Nucleotide Polymorphism (SNP) after quality control. We identified three genomic regions that explained the greatest percentage of the genetic variance, resulting in 25 SNP associated with candidate genes on chromosomes 6, 10, and 21. A total of nine candidate genes are reported for the temperament trait, which is: PYGM, SYVN1, CAPN1, FADS1, SYT7, GRID2, GPRIN3, EEF1A1 and FRY, linked to the energetic activity of the organism, synaptic transmission, meat tenderness, and calcium associated activities. This is the first study to identify these genetic variants associated with temperament in sheep, which could be used as molecular markers in future behavioral research.

show abstract

“…On the other hand, glycogen phosphorylase is one of the most important enzymes involved in the tenderness of meat and may affect the quality of it (Bai et al, 2020a). Therefore, genetic variations could not only affect what is related to stress or welfare, but the stress conditions linked to the end of the productive stage will determine the characteristics of the final product, in this case the meat (Bai et al, 2020a(Bai et al, , 2020b :42,712,976-42,740,799). This gene encodes the μ-calpain 1, a proteolytic enzime with activity on myofibrillar proteins (Speck et al, 1993;Page et al, 2002;Geesink et al, 2006).…”

Section: Chromosomes and Candidate Genesmentioning

confidence: 99%

Identification of Candidate Genes and Pathways Linked to the Temperament Trait in Sheep

Romaniuk,

Vera,

Peraza

et al. 2023

Preprint

View full text Add to dashboard Cite

Temperament can be defined as the emotional variability among animals of the same species in response to the same stimulus, thus being able to group animals by reactivity as nervous, intermediate or calm. The main objective for this study was to identify genomic regions with the temperament phenotype measured by the Isolation Box Test (IBT) by single-step genome wide association studies (ssGWAS). The database used consisted of 4,317 animals with temperament records, and 1,697 genotyped animals with 38,268 effective SNP after quality control. We identified three genomic regions that explained the greatest percentage of the genetic variance, resulting in 25 SNP associated with candidate genes on chromosomes 6, 10 and 21. A total of nine candidate genes are reported for the temperament trait, which are: PYGM, SYVN1, CAPN1, FADS1, SYT7, GRID2, GPRIN3, EEF1A1 and FRY, linked to energetic activity of the organism, synaptic transmission, meat tenderness and calcium associated activities. This is the first study to identify these genetic variants associated with temperament in sheep, leading the opportinuty to potential applications of these molecular markers in future behavioral research.

show abstract

Effects of phosphorylation on the activity of glycogen phosphorylase in mutton during incubation at 4 °C in vitro

Cited by 8 publications

References 40 publications

Protein Phosphorylation Induced by Pyruvate Kinase M2 Inhibited Myofibrillar Protein Degradation in Post-Mortem Muscle

Protein Phosphorylation Induced by Pyruvate Kinase M2 Inhibited Myofibrillar Protein Degradation in Post-Mortem Muscle

Identification of Candidate Genes and Pathways Linked to the Temperament Trait in Sheep

Identification of Candidate Genes and Pathways Linked to the Temperament Trait in Sheep

Contact Info

Product

Resources

About