Effects of Photobiomodulation Therapy with Various Laser Wavelengths on Proliferation of Human Periodontal Ligament Mesenchymal Stem Cells

Abstract

Several methods have been proposed to enhance the regeneration and healing time in periodontal therapy. Photobiomodulation therapy (PBMT) is a recently suggested novel technique for this purpose. This study aimed to compare the efficacy of PBMT with various laser wavelengths and energy densities on proliferation of human periodontal ligament mesenchymal stem cells (PDLMSCs). The wells containing PDLMSCs were subjected to laser irradiation at 635, 660, 808 and 980 nm wavelengths with 1, 1.5, 2.5 and 4 J cm À2 energy densities. Cell proliferation and viability were evaluated after 1, 3 and 5 days with the methyl thiazolyl tetrazolium (MTT) assay and 4,6-diamidino-2-phenylindole (DAPI) staining. No significant difference was observed among the experimental and the control groups on day 1 (P > 0.05). On day 3, 808 nm laser at 4 J cm À2 energy density and 980 nm laser at all densities had significant differences with control group. On day 5, the control group had significant differences in cell proliferation with 808 nm laser at 2.5 and 4 J cm À2 energy densities, and 980 nm laser at all densities. PBMT with 635, 660, 808 and 980 nm wavelengths increased the proliferation of PDLMSCs but the maximum cell viability was prominent after irradiation by 980 nm laser with energy density of 4 J cm À2 on day 3.

Rectifying the impairment of immune thrombocytopenia plasmas through photobiomodulation

“…22 Similar photobiomodulation of 980 nm and 810 nm lasers has been also observed in various cell types, including fetal liver and bone marrow MKs, myoblasts, osteoblasts, periodontal ligament mesenchymal stem cells, keratinocytes, and fibroblasts. 17,[22][23][24] Nevertheless, MKs seem much more sensitive to 980 nm laser than 810 nm laser, making 980 nm laser or LED more appealing for clinical application. This is because it would be more practical to deliver such a low dose of 980 nm laser (0.03 J/cm 2 ) into internal organs noninvasively, in contrast to 810 nm laser, which requires a 100-fold higher energy density (3 J/cm 2 ).…”