Effects of phytase supplementation of high-plant-protein diets on growth, phosphorus utilization, antioxidant, and digestion in red swamp crayfish (Procambarus clarkii)

Also in Crayfish: How Phytase Inclusion Avoids Phytic Acid Effects on Hepatopancreas Enzymes of Redclaw Cherax quadricarinatus

“…Te remaining alkaline protease activity of the redclaw showed PA and phytase's main efects. It agreed with Yang et al [64], who found higher protease activities in red swamp crayfsh (Procambarus clarkii) hepatopancreas with increased phytase doses. Divakaran and Ostrowski [35] indicated that phytase may exert an indirect but positive efect on soybean meal proteolysis, measured as tryptic activity in in vitro assays with white shrimp (L. vannamei) HP extract.…”

“…PA may also have inhibited amylase activity both directly and indirectly, through the chelation of calcium, which is the required cofactor of amylase [72]. Tis supports the existence of energetic benefts due to the inclusion of phytase, as recently found by Yang et al [64] for red swamp crayfsh (P. clarkii) on amylase activity and it was again confrmed here. Suprayudi et al [65] worked with juveniles of the whiteleg shrimp L. vannamei and a 500 FTU/ kg dose from an Aspergillus niger phytase, fnding a 10% improvement in energy digestibility.…”

The application of protease in aquaculture: Prospects for enhancing the aquafeed industry

“…For example, a study on gibel carp showed that 500 mg/kg protease supplementation in LFD could significantly increase hepatic total antioxidant capacity (T-AOC), total superoxide dismutase activities (T-SOD), and glutathione peroxidase activities (GPX) as well as intestinal T-SOD, complement 4, and secretory immunoglobulin A contents ( Xu et al., 2022 ). Yang et al. (2022) confirmed that protease supplementation significantly improved the hemolymph biochemical indices and decreased the contents of malondialdehyde (MDA) in red swamp crayfish.…”

“…(2020) Yellow perch ( Perca flavescens ) 0.59 0.1% pancreatin in basal diets →Trypsin, chymotrypsin, and pepsin Kolkovski et al. (2000b) Crustacean species Red swamp crayfish ( Procambarus clarkii ) 8.18 0, 100, 200, 400, 800, and 1,600 mg/kg of protease (20,000 U/g) added to LFD (FM were replaced by plant protein) ↑Protease both in the intestine and hepatopancreas at 200 and 400 mg/kg protease inclusion levels, ↑Amylase in hepatopancreas at all levels and in the intestine at 200 mg/kg, and ↑Lipase in both intestine and hepatopancreas at all levels, except for at 1,600 mg/kg in the intestine Yang et al. (2022) Chinese mitten crab ( Eriocheir sinensis ) 0.73 0, 125, 150, and 175 mg/kg AG175™ added in LFD (40 g/kg FM replaced with 20 g/kg soybean meal and 40 g/kg CSM) ↑Trypsin in hepatopancreatic tissues at 150 and 175 mg/kg Chowdhury et al.…”

“…In fish, reductions in blood aspartate aminotransferase (AST) and alanine aminotransferase (ALT) have been reported in Nile tilapia ( Hassaan et al., 2019 , 2020 ), gibel carp ( Liu et al., 2018 ), and largemouth bass ( Guan et al., 2021 ), fed with adequate protease supplementation levels. Also, Yang et al. (2022) reported that dietary 200 to 800 mg/kg protease supplementation could significantly decrease the contents of AST and ALT in red swamp crayfish Procambarus clarkia fed plant-based diets.…”