Effects of phytochemicals of Flemingia vestita (Fabaceae) on glucose 6-phosphate dehydrogenase and enzymes of gluconeogenesis in a cestode (Raillietina echinobothrida)

Das, Bidyadhar; Tandon, Veena; Saha, Nirmalendu

doi:10.1016/j.cca.2004.10.004

Cited by 17 publications

(19 citation statements)

References 28 publications

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“…Similar observations were reported by Das et al (2004a) in a cestode Raillietina echinobothrida. MDH activity was found both in cytosolic and mitochondrial fraction, in the present studies, which is in agreement with the observations made by Das et al (2004b) in R. echinobothrida.…”

Section: Discussionsupporting

confidence: 87%

“…The leaf extracts of Ocimum sanctum, Lawsonia inermis and Calotropis gigantea and leaf and flower extracts of Azadirachta indica were found to inhibit MDH in Setaria digitata. Das et al (2004b) reported an increase in the activity of MDH in R. echinobothrida with crude root peel extract of Flemingia vestita whereas activity G-6-PDH was significantly reduced (Das et al 2004a). Artemether, a well known antimalarial drug derived from plant genus Artemesia, exerts a potent inhibitory action on G-6-PDH activity in Schistosoma japonicum (Xiao et al 2004).…”

Section: Discussionmentioning

confidence: 96%

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Effect of Punica granatum fruit peel on glucose-6-phosphate dehydrogenase and malate dehydrogenase in amphistome Gastrothylax indicus

Aggarwal

Bagai

2016

J Parasit Dis

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Increasing anthelmintic resistance and the impact of conventional anthelmintics on the environment, it is important to look for alternative strategies against helminth parasite in sheep. Important lipogenic enzymes like glucose-6-phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase (MDH) show subcellular distribution pattern. Activity of G-6-PDH was largely restricted to cytosolic fraction while MDH was found in both cytosolic and mitochondrial fraction in Following in vitro treatment with ethanolic and aqueous extracts of fruit peel and commercial anthelmintic, albendazole G-6-PDH activity was decreased by 19-32 %, whereas MDH was suppressed by 24-41 %, compared to the respective control. Albendazole was quite effective when compared with negative control and both the extracts. The results indicate that phytochemicals of plant may act as potential vermifuge or vermicide.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 96%

Effect of Punica granatum fruit peel on glucose-6-phosphate dehydrogenase and malate dehydrogenase in amphistome Gastrothylax indicus

Aggarwal

Bagai

2016

J Parasit Dis

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“…Isoflavones are commonly found in soya, with genistein (4Ј,5,7-trihydroxyisoflavone) being the most abundant one. There are very few studies that have examined the role of isoflavones against parasitic helminths (6,18,22). More recently, Gargala et al (10) reported on the proliferation-inhibitory efficacies of epidermal growth factor (EGF) receptor-targeted genistein derivatives against the apicomplexan parasites Sarcocystis neurona, Neospora caninum, and Cryptosporidium parvum.…”

mentioning

confidence: 99%

In Vitro Metacestodicidal Activities of Genistein and Other Isoflavones against Echinococcus multilocularis and Echinococcus granulosus

Naguleswaran

Spicher

Vonlaufen

et al. 2006

Antimicrob Agents Chemother

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Echinococcus multilocularis and Echinococcus granulosus metacestode infections in humans cause alveolar echinococcosis and cystic echinococcosis, respectively, in which metacestode development in visceral organs often results in particular organ failure. Further, cystic hydatidosis in farm animals causes severe economic losses. Although benzimidazole derivatives such as mebendazole and albendazole are being used as therapeutic agents, there is often no complete recovery after treatment. Hence, in searching for novel treatment options, we examined the in vitro efficacies of a number of isoflavones against Echinococcus metacestodes and protoscoleces. The most prominent isoflavone, genistein, exhibits significant metacestodicidal activity in vitro. However, genistein binds to the estrogen receptor and can thus induce estrogenic effects, which is a major concern during long-term chemotherapy. We have therefore investigated the activities of a number of synthetic genistein derivatives carrying a modified estrogen receptor binding site. One of these, Rm6423, induced dramatic breakdown of the structural integrity of the metacestode germinal layer of both species within 5 to 7 days of in vitro treatment. Further, examination of the culture medium revealed increased leakage of parasite proteins into the medium during treatment, but zymography demonstrated a decrease in the activity of metalloproteases. Moreover, two of the genistein derivatives, Rm6423 and Rm6426, induced considerable damage in E. granulosus protoscoleces, rendering them nonviable. These findings demonstrate that synthetic isoflavones exhibit distinct in vitro effects on Echinococcus metacestodes and protoscoleces, which could potentially be exploited further for the development of novel chemotherapeutical tools against larval-stage Echinococcus infection.Echinococcosis is a cosmopolitan zoonotic disease in ungulates and humans that is acquired by infection with the members of the genus Echinococcus at larval stages. Cystic echinococcosis (CE), the causative agent of which is Echinococcus granulosus, is distributed worldwide. In contrast, alveolar echinococcosis (AE) and Echinococcus multilocularis are generally confined to the Northern hemisphere (8). Dogs and foxes are the major definitive hosts (of E. granulosus and E. multilocularis, respectively). After ingestion of eggs, each containing a single oncosphere, by the host, E. granulosus metacestodes normally develop as single fluid-filled unilocular metacestodes, whereas E. multilocularis metacestodes are characterized by exogenous budding and form multilocular conglomerates that exhibit typical features of tumor-like proliferation. Growth and/or proliferation of metacestodes over a long period of time leads to the development of space-occupying lesions, causes organ malfunction, and eventually leads to death (14,26).The preferred treatment strategy for CE and AE is radical resection of the parasitic mass (26). However, in inoperable cases, chemotherapy is the only option. Benzimidazole carbamate deri...

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“…Such distortions may be attributed to tegumental enzymes since they are the primary target of such actions (Das et al 2004;Dasgupta et al 2010). Further, the present study also revealed infoldings on the edge of proglottid which may be due to an increase in chloride ion conductance of worm muscle membrane producing hyperpolarization and reducing excitability that could lead to muscle relaxation and flaccid paralysis (Martin 1985).…”

Section: Discussionmentioning

confidence: 77%

Senna alexandrina Mill. induced ultrastructural changes on Hymenolepis diminuta

et al. 2016

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Mill. has been used for antimicrobial activity. In the present study, the crude ethanolic extract of the plant and a synthetic compound Sennoside were tested in vitro on to evaluate its potential anthelmintic efficacy through ultrastructural changes. Worms were maintained between rat model and beetle and the test parasites were exposed to different concentrations of crude ethanolic leaf extracts of. Praziquantel was used as a reference drug. Dose dependent efficacy was observed in terms of motility and time of mortality in all treated parasites. Ultrastructural micrography revealed irrevocable destruction all over the body tegument accompanied with sloughing of microtriches and swellings of the basal lamina. Vacuolization of the syncytium along with sparsely cytoplasmic cytons and depletion of parenchymatous layer were observed accompanied by deformities in the cell organelles. Extensive deformities in the tegument indicates that the plant extract alter membrane permeability of the parasite leading to paralysis and subsequent death. Thus, can be regarded as a potential anthelmintic agent.

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Effects of phytochemicals of Flemingia vestita (Fabaceae) on glucose 6-phosphate dehydrogenase and enzymes of gluconeogenesis in a cestode (Raillietina echinobothrida)

Cited by 17 publications

References 28 publications

Effect of Punica granatum fruit peel on glucose-6-phosphate dehydrogenase and malate dehydrogenase in amphistome Gastrothylax indicus

Effect of Punica granatum fruit peel on glucose-6-phosphate dehydrogenase and malate dehydrogenase in amphistome Gastrothylax indicus

In Vitro Metacestodicidal Activities of Genistein and Other Isoflavones against Echinococcus multilocularis and Echinococcus granulosus

Senna alexandrina Mill. induced ultrastructural changes on Hymenolepis diminuta

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