Effects of phytohormones on mycelial growth and exopolysaccharide biosynthesis of medicinal mushroom Pellinus linteus

Abstract

Effects of phytohormones including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic [corrected] acid (NAA) on mycelial growth of medicinal mushroom Phellinus linteus were investigated. Under the optimal IAA, IBA and NAA concentration of 1.0, 1.5 and 5.0 mg/l, the maximal mycelial diameter reached 8.6 +/- 0.4, 7.3 +/- 2.6 and 9.0 +/- 1.0 mm, respectively. The mycelial biomass and exopolysaccharide (EPS) production with addition of 5.0 mg/l NAA in a shake flask were 6.24 +/- 0.18 g/l at 168 h and 0.86 +/-0.01 g/l at 192 h, which were enhanced by 15.98 and 56.36% compared to the control, respectively. However, the molecular weight and infrared spectrum of EPS were coincident with the control. Results indicated that NAA at the proper concentration was beneficial in stimulating mycelial growth and EPS biosynthesis, whereas it could not alter the molecular structure of EPS.

Effect of heavy metal-solubilizing microorganisms on zinc and cadmium extractions from heavy metal contaminated soil with Tricholoma lobynsis

“…Regrettably, neither of the isolated strains in our study possessed ACC deaminase abilities. Phytohormones had been reported to stimulate mycelial growth and enhance biomass (Guo et al 2009). The maximum IAA production of both M6 and K1 strains were observed after 36 h cultivation in SMS medium supplemented with 0.5 mg ml -1 of tryptophane (Fig.…”

“…Other macrofungi, non-ectomycorrhizal, are also reported to have high accumulation and bioconcentration abilities (Demirbas 2002;Kalac and Svoboda 2000;Mendil et al 2005), which can be exploited for removing high metal from the soil. There are only a few reports about the interactions of macrofungi and its associated microbes in metal contaminated soil, though related studies, such as mushroom growth promoted by microbe treatment (Han 1999), mycelial growth enhanced by phytohormones (Guo et al 2009) and primordia formation influenced by related microbes in the soil (Cho et al 2003), have been documented.…”

Influence of Phytohormones on Monosaccharide Composition of Polysaccharides from Wheat Suspension Culture

“…Arabinans are known to play role in cell-to-cell adhesion [21], mechanical property of the cell wall [22], endo-arabinase activity [23]. It was determined that ABA induce fast and enhanced induction of reactive oxygen species and altered expression of cell wall modification genes, including pectin-esterase and xyloglucan enotransglycosylase [16]. It was reported that ABA is able to induce expression of cell wall loosening gene in Arabidopsis [24].…”

“…Recent studies have shown that auxins are able to enhance susceptibility to bacterial invasion [14] and induce upregulation of biosynthetic genes [15]. It was reported that abscisic acid is capable of altering cell wall properties and compositions [16].…”

Macrofungi: A review of cultivation strategies, bioactivity, and application of mushrooms

“…Provided that there are sufficient numbers of high-quality spores, the substrate composition, pH, moisture, inoculation density, and carbon-to-nitrogen ratio (C/N) may dramatically influence mycelial biomass and bioactive products (Bellettini et al, 2019;Yang, Min, Bi, Zhou, & Huang, 2013). The constituents of the culture medium influence Yang, Wu, and Zhang (2004) Pellinus linteus Phytohormones: indole-3-acetic acid, indole-3-butyric acid, and 1-naphthaleneacetic acid at 1 mg/L, 1.5 mg/L, and 5 mg/L, respectively Mycelial growth and EPS Guo, Zou, and Sun (2009) Abbreviations: BIO, biomass; EPS, exopolysaccharide; IPS, intracellular polysaccharide. macrofungus safety.…”