Effects of Piperonyl Butoxide on the Accumulation of Lipid and the Transcript Levels of DtMFPα in Dunaliella tertiolecta

Abstract

As one of the sources of biodiesel, microalgae are expected to solve petroleum shortage. In this study, different concentrations of piperonyl butoxide were added to the culture medium to investigate their effects on the growth, pigment content, lipid accumulation, and content of carotenoids in Dunaliella tertiolecta. The results showed that piperonyl butoxide addition significantly decreased the biomass, chlorophyll content, and total carotenoid content but hugely increased the lipid accumulation. With the treatment of 150 ppm piperonyl butoxide combined with 8000 Lux light intensity, the final lipid accumulation and single-cell lipid content were further increased by 21.79 and 76.42% compared to those of the control, respectively. The lipid accumulation in D. tertiolecta is probably related to the increased expression of DtMFPα in D. tertiolecta under the action of piperonyl butoxide. The phylogenetic trees of D. tertiolecta and other oil-rich plants were constructed by multiple sequence alignment of DtMFPα, demonstrating their evolutionary relationship, and the tertiary structure of DtMFPα was predicted. In conclusion, piperonyl butoxide has a significant effect on lipid accumulation in D. tertiolecta, which provides valuable insights into chemical inducers to enhance biodiesel production in microalgae to solve the problem of diesel shortage.

Functional Characterization of a CruP-Like Isomerase in Dunaliella

“…pZBEL-GP was preserved in our laboratory and used for the construction of gene overexpression in Dunaliella. 32 We inserted DbLyISO gene fragment to pRSFDuet-1 (NcoI-EcoRI) to construct pRSFDuet-LyISO. Plasmid multiplication was performed using E. coli DH5α as the cloning host.…”

Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

“…The upper layer was the aqueous phase containing NaCl and other water-soluble substances, and the lower layer was the chloroform layer to be collected. Chloroform evaporated in an oven, the remaining was weighed, and the production (g/L) and content (%) of lipid are calculated according to the following formulae P lipid 0.25em ( g / L ) = W lipid / V algae C lipid 0.25em ( % ) = W lipid / W algae where P lipid is the lipid production, W lipid is the dry weight of lipid, V algae is the volume of algal solution taken, C lipid is the lipid content, and W algae is the dry weight of algal cells.…”

Driving into the Factory of Docosahexaenoic Acid (DHA), Microalgae

“…For instance, the addition of stimulators such as 2-phenylacetic acid (PAA) improves the growth, DHA, and the relevant transcriptome in Aurantiochytrium sp. [111]. This method is considered practical and efficient without any need to comprehensive understanding of the molecular targets.…”