Effects of pituitary adenylate cyclase activating polypeptide on small intestinal INT 407 cells

Illés, Anita; Opper, Balázs; Reglődi, Dóra; Kerényi, Mónika; Czétány, Péter; Boronkai, Árpád; Schäfer, Eszter; Tóth, Gábor; Fábián, Eszter; Horváth, Gabriella

doi:10.1016/j.npep.2017.07.002

Cited by 10 publications

(8 citation statements)

References 61 publications

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“…Kasica et al [ 39 ] showed that PACAP has an anti-apoptotic effect on zebrafish hair cells by reducing the cleaved caspase-3 level and reducing oxidative stress. Furthermore, the antioxidative effect of PACAP on small intestine INT 407 cells was also shown [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Diabetes Affects the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)-Like Immunoreactive Enteric Neurons in the Porcine Digestive Tract

Palus

Bulc

Całka

et al. 2021

IJMS

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Diabetic gastroenteropathy is a common complication, which develops in patients with long-term diabetes. The pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide known for its cytoprotective properties and plays an important role in neuronal development, neuromodulation and neuroprotection. The present study was designed to elucidate, for the first time, the impact of prolonged hyperglycaemia conditions on a population of PACAP-like immunoreactive neurons in selected parts of the porcine gastrointestinal tract. The experiment was conducted on 10 juvenile female pigs assigned to two experimental groups: The DM group (pigs with streptozocin-induced diabetes) and the C group (control pigs). Diabetes conditions were induced by a single intravenous injection of streptozocin. Six weeks after the induction of diabetes, all animals were euthanised and further collected, and fixed fragments of the stomach, duodenum, jejunum, ileum and descending colon were processed using the routine double-labelling immunofluorescence technique. Streptozotocin-induced hyperglycaemia caused a significant increase in the population of PACAP-containing enteric neurons in the porcine stomach, small intestines and descending colon. The recorded changes may result from the direct toxic effect of hyperglycaemia on the ENS neurons, oxidative stress or inflammatory conditions accompanying hyperglycaemia and suggest that PACAP is involved in regulatory processes of the GIT function in the course of diabetes.

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Section: Discussionmentioning

confidence: 99%

Diabetes Affects the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)-Like Immunoreactive Enteric Neurons in the Porcine Digestive Tract

Palus

Bulc

Całka

et al. 2021

IJMS

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“…An increasing number of reports have confirmed the involvement of PACAP in the regulation of pathological processes in the GI tract. PACAP has been shown to have anti-inflammatory, antioxidant and cytoprotective effects [7,[10][11][12][13][14][15][29][30][31][92][93][94][95][96][97][98][99][100][101][102][103][104][105][106][107][108][109]. The involvement of PACAP in the regulation of gastrointestinal disorders is presented below and summarized in Table 2.…”

Section: Pacap Participation In Pathological Processes In the Gi Tractmentioning

confidence: 99%

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in Physiological and Pathological Processes within the Gastrointestinal Tract: A Review

Karpiesiuk

Palus

2021

IJMS

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Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide widely distributed in the central nervous system (CNS) and many peripheral organs, such as the digestive tract, endocrine, reproductive and respiratory systems, where it plays different regulatory functions and exerts a cytoprotective effect. The multifarious physiological effects of PACAP are mediated through binding to different G protein-coupled receptors, including PAC1 (PAC1-R), VPAC1 (VPAC1-R) and VPAC2 (VPAC2-R) receptors. In the gastrointestinal (GI) tract, PACAP plays an important regulatory function. PACAP stimulates the secretion of digestive juices and hormone release, regulates smooth muscle contraction, local blood flow, cell migration and proliferation. Additionally, there are many reports confirming the involvement of PACAP in pathological processes within the GI tract, including inflammatory states, neuronal injury, diabetes, intoxication and neoplastic processes. The purpose of this review is to summarize the distribution and pleiotropic action of PACAP in the control of GI tract function and its cytoprotective effect in the course of GI tract disorders.

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“…PACAP also has cytoprotective effects in different non-neuronal cells, such as endothelial cells, intestinal cells or pinealocytes [11,16,21,22].…”

Section: Text: Introductionmentioning

confidence: 99%

Protective Role of Endogenous PACAP in Inflammation-induced Retinal Degeneration

Vaczy

Kovari

Kovács

et al. 2018

CPD

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Purpose. Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuroprotective peptide that has been shown to exert protective effects in different models of neurodegenerative diseases, including retinal degenerations. Data obtained from PACAPdeficient (PACAP KO) mice provide evidence that endogenous PACAP has neuroprotective role in different pathologies. PACAP KO mice show enhanced sensitivity to different insults, such as oxidative stress, hypoxia and inflammation. The aim of the present study was to investigate the protective effects of endogenous PACAP in retinal inflammation. Methods. Endotoxin-caused eye inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS) in PACAP KO and wild type (Wt) mice. After LPS treatment, retinas were processed for histological examination. To detect the alterations of different proteins and cytokines, immunohistochemical, western blot and cytokine array were used. We also performed dark-adapted electroretinography (ERG) to detect the functional differences. Results. The thickness of nearly all layers was significantly less in LPS-injected PACAP KO mice compared to Wt animals. Increased expression of glial fibrillary acidic protein (GFAP) was induced in Müller glial cells after LPS treatment, which was more intense in PACAP KO mice. The levels of pAkt and pGSK were decreased in PACAP KO group during inflammation. LPS treatment significantly increased cytokines (sICAM-1, JE, TIMP-1) in both treated groups, but it was more expressed in PACAP KO animals. Furthermore, ERG responses were disturbed after LPS injection in PACAP KO mice. Conclusion. Our results showed that endogenous PACAP has a protective role in LPS-caused retinal inflammation.

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Effects of pituitary adenylate cyclase activating polypeptide on small intestinal INT 407 cells

Cited by 10 publications

References 61 publications

Diabetes Affects the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)-Like Immunoreactive Enteric Neurons in the Porcine Digestive Tract

Diabetes Affects the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP)-Like Immunoreactive Enteric Neurons in the Porcine Digestive Tract

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in Physiological and Pathological Processes within the Gastrointestinal Tract: A Review

Protective Role of Endogenous PACAP in Inflammation-induced Retinal Degeneration

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