Effects of Pituitary Hormones and NADPH on Acetate Utilization in Ovarian and Adrenocortical Tissues1

Conversion of Cholesterol to Progesterone by Corpus Luteum Slices

“…{Endocrinology 75: 215, 1964) I N THE COURSE of recent studies on the corpus luteum, an array of biochemical and enzymatic properties have emerged which bear a striking resemblance to those of the adrenal cortex; these have recently been reviewed (1,2). The pituitary luteinizing hormone, LH, 3 in its specific stimulation of steroido genesis in the corpus luteum slice (3,4), causes increased incorporation of acetate-l-14 C into progesterone (2), and in this respect acts in a manner similar to that of ACTH in the adrenal cortex slice (2,5). The nucleotide cofactor, NADPH, Received January 23,1964.…”

“…1 Investigator, Howard Hughes Medical Institute. 2 Present address: Lilly Research Laboratories, Eli Lilly and Company, Indianapolis 6, Indiana. s The abbreviations used are: LH, luteinizing hormone; ACTH, adrenocorticotropic hormone; NADP and NADPH, oxidized and reduced nicotinamide adenine dinucleotide phosphate; glucose-6-P or G-6-P, glucose-6-phosphate.…”

“…s The abbreviations used are: LH, luteinizing hormone; ACTH, adrenocorticotropic hormone; NADP and NADPH, oxidized and reduced nicotinamide adenine dinucleotide phosphate; glucose-6-P or G-6-P, glucose-6-phosphate. considered to be a mediator of ACTH action in steroidogenesis (6), fails to increase the utilization of acetate-l-14 C in the course of its stimulation of steroidogenesis in both tissues (2).…”

Factors Influencing the Secretion of Testosterone in the Anesthetized Dog

“…The data of Fig. 2 thus demonstrate that the canine testis in vivo behaves biosynthetically like the rabbit testis (7), and the bovine corpus luteum in vitro (18). It is currently the opinion of many investigators that the pituitary gonadotrophins increase steroid biosynthesis in the ovary and testis by activating the phosphorylase concentration in these tissues.…”

Mechanisms of aging in relationship to steroid biotransformations and the thiobarbituric acid reactive material in rate testicular tissue in vitro

“…On the contrary, many of the investigations mentioned above were carried out in the presence of NADPH or a NADPH-generating system. This experimental difference is very important in the light of the reports by Savard and Casey (1964), Berliner, Ellis, and Taylor (1964), and Ellis and Berliner (1964), who showed an increase in the production of androgens with the addition of NADPH or its generating system to the incubation mixture.…”