Effects of plant antimicrobial phenolic compounds on virulence of the genus Pectobacterium

Joshi, Janak Raj; Burdman, Saul; Lipsky, Alexander; Yedidia, Iris

doi:10.1016/j.resmic.2015.04.004

Cited by 59 publications

(48 citation statements)

References 44 publications

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“…Recently, we have shown that non‐lethal concentrations of various phenolic compounds affect PCWDE activity, biofilm formation and the motility of several Pectobacterium species, as well as their virulence in interactions with various host plants (Joshi et al ., ). In the present study, we investigated the effects of two phenolic acids, CA and SA, on the expression of several virulence‐associated genes in pectobacteria.…”

Section: Discussionmentioning

confidence: 97%

“…Moreover, SA has been found to reduce infection in tissue‐cultured potato infected by Dickeya solani (Czajkowski et al ., ). In agreement with these findings, we have shown recently that non‐lethal concentrations of several plant phenolic compounds, including SA and cinnamic acid (CA), affect several virulence‐associated traits and reduce the virulence of several Pectobacterium strains in different hosts (Joshi et al ., ). To unravel the mechanism by which plant secondary metabolites, such as CA and SA, affect soft rot bacterial virulence, we investigated the effect of these compounds on the expression patterns of selected virulence‐related genes in Pectobacterium aroidearum and P. carotovorum ssp.…”

Section: Introductionmentioning

confidence: 97%

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Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Joshi

Burdman

Lipsky

et al. 2015

Molecular Plant Pathology

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Several studies have reported effects of the plant phenolic acids cinnamic acid (CA) and salicylic acid (SA) on the virulence of soft rot enterobacteria. However, the mechanisms involved in these processes are not yet fully understood. Here, we investigated whether CA and SA interfere with the quorum sensing (QS) system of two Pectobacterium species, P. aroidearum and P. carotovorum ssp. brasiliense, which are known to produce N-acyl-homoserine lactone (AHL) QS signals. Our results clearly indicate that both phenolic compounds affect the QS machinery of the two species, consequently altering the expression of bacterial virulence factors. Although, in control treatments, the expression of QS-related genes increased over time, the exposure of bacteria to non-lethal concentrations of CA or SA inhibited the expression of QS genes, including expI, expR, PC1_1442 (luxR transcriptional regulator) and luxS (a component of the AI-2 system). Other virulence genes known to be regulated by the QS system, such as pecS, pel, peh and yheO, were also down-regulated relative to the control. In agreement with the low levels of expression of expI and expR, CA and SA also reduced the level of the AHL signal. The effects of CA and SA on AHL signalling were confirmed in compensation assays, in which exogenous application of N-(β-ketocaproyl)-l-homoserine lactone (eAHL) led to the recovery of the reduction in virulence caused by the two phenolic acids. Collectively, the results of gene expression studies, bioluminescence assays, virulence assays and compensation assays with eAHL clearly support a mechanism by which CA and SA interfere with Pectobacterium virulence via the QS machinery.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Joshi

Burdman

Lipsky

et al. 2015

Molecular Plant Pathology

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“…Thereafter, those concentrations of both phenolic volatiles were added with eAHL (100 nM) to study the effect in soft rot symptoms. The experiment was performed on fully expanded young leaves of cabbage and calla lily and small (about 25–40 g) potato tubers as previously described47.…”

Section: Methodsmentioning

confidence: 99%

Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins

Joshi

Khazanov

Senderowitz

et al. 2016

Sci Rep

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Quorum sensing (QS) is a population density-dependent regulatory system in bacteria that couples gene expression to cell density through accumulation of diffusible signaling molecules. Pectobacteria are causal agents of soft rot disease in a range of economically important crops. They rely on QS to coordinate their main virulence factor, production of plant cell wall degrading enzymes (PCWDEs). Plants have evolved an array of antimicrobial compounds to anticipate and cope with pathogens, of which essential oils (EOs) are widely recognized. Here, volatile EOs, carvacrol and eugenol, were shown to specifically interfere with QS, the master regulator of virulence in pectobacteria, resulting in strong inhibition of QS genes, biofilm formation and PCWDEs, thereby leading to impaired infection. Accumulation of the signal molecule N-acylhomoserine lactone declined upon treatment with EOs, suggesting direct interaction of EOs with either homoserine lactone synthase (ExpI) or with the regulatory protein (ExpR). Homology models of both proteins were constructed and docking simulations were performed to test the above hypotheses. The resulting binding modes and docking scores of carvacrol and eugenol support potential binding to ExpI/ExpR, with stronger interactions than previously known inhibitors of both proteins. The results demonstrate the potential involvement of phytochemicals in the control of Pectobacterium.

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“…Similarly, salicyclic acid, cinnamic acid, coumaric acid, halogenated furanones and N,N′-alkylated imidazolium-derivatives were reported as QSI compound specifically target against QS system of Pectobacterium spp. (Bodini et al 2009;des Essarts et al 2013;Joshi et al 2015aJoshi et al , 2015bLagonenko et al 2013;Manefield et al 2001). Also, several studies suggested that the development of QSI strategy is one of the most promising approaches to control the bacterial infections (Defoirdt et al 2013;Galloway et al 2012;Helman and Chernin 2015;Kalia 2013).…”

Section: Introductionmentioning

confidence: 99%

Curcumin from Curcuma longa affects the virulence of Pectobacterium wasabiae and P. carotovorum subsp. carotovorum via quorum sensing regulation

et al. 2016

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In plant soft-rotting bacteria Pectobacterium, quorum sensing (QS) regulates the secretion of an arsenal of plant cell wall degrading extracellular enzymes (PCWDEs) and flagella-mediated motility via two different signaling molecules such as 3-oxohexanoyl-Lhomoserine lactone (3-oxo-C6-AHL) and 3-oxooctanoyl-L-homoserine lactone (3-oxo-C8-AHL). In the present investigation, the phytochemical compound curcumin was assessed for its QS inhibitory potential against AHL-dependent PCWDEs production and motility in P. wasabiae SCC3193, P. carotovorum subsp. Carotovorum Pcc21 and P. carotovorum subsp. Carotovorum Pcc. Interestingly, curcumin at sub-MIC effectively inhibited the production of PCWDEs as well as the swimming and swarming motility of all tested pathogens in a non-bactericidal fashion. Subsequently, the in vitro root pathogenicity assay in Arabidopsis thaliana unveiled the rescuing efficacy of curcumin against the pathogenicity of tested plant pathogens by attenuating its QS mediated virulence factors production. Besides, studies with QS mutant strain Pcc21-M10 also evidenced the QSI property of curcumin.

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Effects of plant antimicrobial phenolic compounds on virulence of the genus Pectobacterium

Cited by 59 publications

References 44 publications

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum ssp. brasiliense via quorum sensing regulation

Plant phenolic volatiles inhibit quorum sensing in pectobacteria and reduce their virulence by potential binding to ExpI and ExpR proteins

Curcumin from Curcuma longa affects the virulence of Pectobacterium wasabiae and P. carotovorum subsp. carotovorum via quorum sensing regulation

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